Examination of Triacylglycerol Biosynthetic Pathways via De Novo Transcriptomic and Proteomic Analyses in an Unsequenced Microalga
Open accessAbstract
Biofuels derived from algal lipids represent an opportunity to dramatically impact the global energy demand for transportation fuels. Systems biology analyses of oleaginous algae could greatly accelerate the commercialization of algal-derived biofuels by elucidating the key components involved in lipid productivity and leading to the initiation of hypothesis-driven strain-improvement strategies. However, higher-level systems biology analyses, such as transcriptomics and proteomics, are highly dependent upon available genomic sequence data, and the lack of these data has hindered the pursuit of such analyses for many oleaginous microalgae. In order to examine the triacylglycerol biosynthetic pathway in the unsequenced oleaginous microalga, Chlorella vulgaris, we have established a strategy with which to bypass the necessity for genomic sequence information by using the transcriptome as a guide. Our results indicate an upregulation of both fatty acid and triacylglycerol biosynthetic machinery under oil-accumulating conditions, and demonstrate the utility of a de novo assembled transcriptome as a search model for proteomic analysis of an unsequenced microalga.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In Chlorella vulgaris, core metabolic model targeting lipid accumulation increased lipid content.
“| 5 | Chlorella vulgaris | 39.08 | Core metabolic model | Lipid accumulation | Lipid quantification, growth rate analysis | Endogenous promoters or heterologous expression constructs | Increased lipid content | Biofuel production, nutritional supplements | [93] |”
From A Holistic Approach to Circular Bioeconomy Through the Sustainable Utilization of Microalgal Biomass for Biofuel and Other Value-Added Products (2024) · cites this paper as [93]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Conesa, A., Götz, S., Garcia-Gomez, J. M., Terol, J., Talón, M., & Robles, M. (2005) · Bioinformatics · doi:10.1093/bioinformatics/bti610 · Cited by 11,417 · see citing works
Velvet: Algorithms for de novo short read assembly using de Bruijn graphsPreprint
Zerbino, D. R., & Birney, E. (2008) · Genome Research · doi:10.1101/gr.074492.107 · Cited by 9,387 · see citing works
Applied and Environmental Microbiology
(2018) · Applied and Environmental Microbiology · doi:10.1128/eissn.1098-5336 · Cited by 5,287 · see citing works
Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances
Hu, Q., Sommerfeld, M., Jarvis, E., Ghirardi, M., Posewitz, M., Seibert, M., & Darzins, A. (2008) · The Plant Journal · doi:10.1111/j.1365-313x.2008.03492.x · Cited by 3,745 · see citing works
Self-citationDirect transesterification of all classes of lipids in a one-step reaction.
Lepage, G., & ROY, C. C. (1986) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)38861-1 · Cited by 2,385 · see citing works
Pfam: clans, web tools and services
FINN, R. (2005) · Nucleic Acids Research · doi:10.1093/nar/gkj149 · Cited by 2,307 · see citing works
Falkowski, P. G., & Raven, J. A. (2007) · Princeton University Press eBooks · doi:10.1515/9781400849727 · Cited by 1,958 · see citing works
An outlook on microalgal biofuels
Wijffels, R. H., & Barbosa, M. J. (2010) · Science · doi:10.1126/science.1189003 · Cited by 1,886 · see citing works
Ohlrogge, J., & Browse, J. (1995) · The Plant Cell · doi:10.1105/tpc.7.7.957 · Cited by 1,492 · see citing works
Lipid productivity as a key characteristic for choosing algal species for biodiesel production
Griffiths, M. J., & Harrison, S. T. L. (2009) · Journal of Applied Phycology · doi:10.1007/s10811-008-9392-7 · Cited by 1,421 · see citing works
Biodiesel from algae: challenges and prospects
Scott, S. A., Davey, M. P., Dennis, J. S., Horst, I., Howe, C. J., Lea-Smith, D. J., & Smith, A. G. (2010) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2010.03.005 · Cited by 1,183 · see citing works
Genetic Engineering of Algae for Enhanced Biofuel Production
Radakovits, R., Jinkerson, R. E., Darzins, A., & Posewitz, M. C. (2010) · Eukaryotic Cell · doi:10.1128/ec.00364-09 · Cited by 1,142 · see citing works
Self-citationStatistical Analysis of Membrane Proteome Expression Changes in Saccharomyces cerevisiae
Zybailov, B. L., Mosley, A. L., Sardiu, M. E., Coleman, M. K., Florens, L. A., & Washburn, M. P. (2006) · Journal of Proteome Research · doi:10.1021/pr060161n · Cited by 1,031 · see citing works
Siaut, M., Cuiné, S., Cagnon, C., Fessler, B., Nguyen, H. M., Carrier, P., Beyly, A., Beisson, F., Triantaphylidès, C., Li‐Beisson, Y., et al. (2011) · BMC Biotechnology · doi:10.1186/1472-6750-11-7 · Cited by 754 · see citing works
Annual review of plant physiology and plant molecular biology
Briggs, W. R., Jones, R. L., Walbot, V., Somerville, C. R., & Delmer, D. P. (1988) · Medical Entomology and Zoology · Cited by 696 · see citing works
Regulation of fatty acid synthesis and oxidation by the AMP-activated protein kinase
Hardie, D. G., & Pan, D. A. (2002) · Biochemical Society Transactions · doi:10.1042/bst0301064 · Cited by 561 · see citing works
Miller, R., Wu, G., Deshpande, R. R., Vieler, A., Gärtner, K., Li, X., Moellering, E. R., Zäuner, S., Cornish, A. J., Liu, B., et al. (2010) · PLANT PHYSIOLOGY · doi:10.1104/pp.110.165159 · Cited by 511 · see citing works
Blanc, G., Duncan, G. A., Agarkova, I., Borodovsky, M. Y., Gurnon, J. R., Kuo, A., Lindquist, E., Lucas, S. M., Pangilinan, J. L., Polle, J. E. W., et al. (2010) · The Plant Cell · doi:10.1105/tpc.110.076406 · Cited by 504 · see citing works
Diacylglycerol kinases: at the hub of cell signalling
Mérida, I., Ávila‐Flores, A., & Merino, E. (2007) · Biochemical Journal · doi:10.1042/bj20071040 · Cited by 427 · see citing works
Lipid production of Chlorella vulgaris cultured in artificial wastewater medium
Feng, Y., Li, C., & Zhang, D. (2010) · Bioresource Technology · doi:10.1016/j.biortech.2010.06.016 · Cited by 424 · see citing works
Moellering, E. R., & Christoph Benning, A. (2009) · Eukaryotic Cell · doi:10.1128/ec.00203-09 · Cited by 401 · see citing works
Work, V. H., Radakovits, R., Jinkerson, R. E., Meuser, J. E., Elliott, L. G., Vinyard, D. J., Laurens, L. M. L., Dismukes, G. C., & Posewitz, M. C. (2010) · Eukaryotic Cell · doi:10.1128/ec.00075-10 · Cited by 351 · see citing works
Anaerobic Acclimation in Chlamydomonas reinhardtii
Mus, F., Dubini, A., Seibert, M., Posewitz, M. C., & Grossman, A. R. (2007) · Journal of Biological Chemistry · doi:10.1074/jbc.m701415200 · Cited by 316 · see citing works
Self-citationTargeting of the Arabidopsis Homomeric Acetyl-Coenzyme A Carboxylase to Plastids of Rapeseeds
Roesler, K. R., Shintani, D. K., Savage, L., Boddupalli, S., & Ohlrogge, J. B. (1997) · PLANT PHYSIOLOGY · doi:10.1104/pp.113.1.75 · Cited by 307 · see citing works
Rismani‐Yazdi, H., HAZNEDAROĞLU, B. Z., Bibby, K. J., & Peccia, J. (2011) · BMC Genomics · doi:10.1186/1471-2164-12-148 · Cited by 295 · see citing works
González-Ballester, D., Casero, D., Cokus, S. J., Pellegrini, M. A., Merchant, S., & Grossman, A. R. (2010) · The Plant Cell · doi:10.1105/tpc.109.071167 · Cited by 268 · see citing works
A Comparison of Labeling and Label-Free Mass Spectrometry-Based Proteomics Approaches
Patel, V. J., Thalassinos, K., Slade, S. E., Connolly, J. B., Crombie, A. T., Murrell, J. C., & Scrivens, J. H. (2009) · Journal of Proteome Research · doi:10.1021/pr900080y · Cited by 259 · see citing works
Poulsen, N., & Kröger, N. (2005) · The FEBS Journal · doi:10.1111/j.1742-4658.2005.04760.x · Cited by 229 · see citing works
Tong, W., Cao, X. M., Harris, S., Sun, H., Fang, H., Fuscoe, J. C., Harris, A. J., Hong, H., Xie, Q., Perkins, R., et al. (2003) · Environmental Health Perspectives · doi:10.1289/ehp.6497 · Cited by 219 · see citing works
Regulation of Chloroplast Gene Expression
Mayfield, S. P., Yohn, C. B., Cohen, A., & Danon, A. (1995) · Annual Review of Plant Physiology and Plant Molecular Biology · doi:10.1146/annurev.pp.46.060195.001051 · Cited by 196 · see citing works
Post-transcriptional regulation of chloroplast gene expression in Chlamydomonas reinhardtii
Rochaix, J.-D. (1996) · Plant Molecular Biology · doi:10.1007/bf00039389 · Cited by 191 · see citing works
SUGAR COMPOSITION OF THE CELL WALL AND THE TAXONOMY OF CHLORELLA (CHLOROPHYCEAE)1
Takeda, H. (1991) · Journal of Phycology · doi:10.1111/j.0022-3646.1991.00224.x · Cited by 179 · see citing works
Dittami, S. M., Scornet, D., Petit, J., Segurens, B., Silva, C. D., Corre, E., Dondrup, M., Glatting, K., König, R., Sterck, L., et al. (2009) · Genome biology · doi:10.1186/gb-2009-10-6-r66 · Cited by 173 · see citing works
Stephenson, A. L., Dennis, J. S., Howe, C. J., Scott, S. A., & Smith, A. G. (2010) · Biofuels · doi:10.4155/bfs.09.1 · Cited by 162 · see citing works
KIRK, M., & KIRK, D. (1985) · Cell · doi:10.1016/s0092-8674(85)80015-5 · Cited by 155 · see citing works
Control Mechanisms of Plastid Gene Expression
Gruissem, W., & Tonkyn, J. C. (1993) · Critical Reviews in Plant Sciences · doi:10.1080/713608040 · Cited by 149 · see citing works
TRANSLATIONAL REGULATION OF GENE EXPRESSION IN CHLOROPLASTS AND MITOCHONDRIA
Gillham, N. W., Boynton, J. E., & Hauser, C. R. (1994) · Annual Review of Genetics · doi:10.1146/annurev.ge.28.120194.000443 · Cited by 129 · see citing works
Omics in algae: Paving the way for a systems biological understanding of algal stress phenomena?
Jamers, A., Blust, R., & Coen, W. D. (2009) · Aquatic Toxicology · doi:10.1016/j.aquatox.2009.02.012 · Cited by 120 · see citing works
Dubini, A., Mus, F., Seibert, M., Grossman, A. R., & Posewitz, M. C. (2009) · Journal of Biological Chemistry · doi:10.1074/jbc.m803917200 · Cited by 108 · see citing works
Self-citationTransient expression of firefly luciferase in protoplasts of the green alga Chlorella ellipsoidea
Jarvis, E. E., & Brown, L. M. (1991) · Current Genetics · doi:10.1007/bf00355062 · Cited by 105 · see citing works
Electrotransformation of Chlorella vulgaris
Chow, K.-C., & Tung, W. L. (1999) · Plant Cell Reports · doi:10.1007/s002990050660 · Cited by 103 · see citing works
North American Journal of Aquaculture
(2011) · Fisheries · doi:10.1577/03632415.2011.10389049 · Cited by 100 · see citing works
Wienkoop, S., Weiß, J., May, P., Kempa, S., Irgang, S., Recuenco-Muñoz, L., Pietzke, M., Schwemmer, T., Rupprecht, J., Egelhofer, V., et al. (2010) · Molecular BioSystems · doi:10.1039/b920913a · Cited by 99 · see citing works
Cell wall proteomics of the green alga Haematococcus pluvialis (Chlorophyceae)
Wang, S.-B., Hu, Q., Sommerfeld, M. R., & Chen, F. (2004) · PROTEOMICS · doi:10.1002/pmic.200300634 · Cited by 95 · see citing works
Dyhrman, S. T., Haley, S. T., Birkeland, S. R., Wurch, L. L., Cipriano, M. J., & McArthur, A. G. (2006) · Applied and Environmental Microbiology · doi:10.1128/aem.72.1.252-260.2006 · Cited by 87 · see citing works
Scheler, C., Li, X., Salnikow, J., Dunn, M. J., & Jungblut, P. R. (1999) · Electrophoresis · doi:10.1002/(sici)1522-2683(19991201)20:18 · Cited by 72 · see citing works
KIM, Y. K., Yoo, W., Lee, S. H., & LEE, M. Y. (2005) · Ecotoxicology · doi:10.1007/s10646-005-0009-5 · Cited by 65 · see citing works
Aizzat, O., Yap, S., Sopiah, H., Madiha, M., Hazreen, M., Shailah, A., Junizam, W. W., Syaidah, A. N., Srijit, D., Musalmah, M., et al. (2010) · Advances in Medical Sciences · doi:10.2478/v10039-010-0046-z · Cited by 56 · see citing works
Characteristics of the Gene That Encodes Acetyl‐CoA Carboxylase in the Diatom Cyclotella crypticaa
Roessler, P. G., Bleibaum, J. L., Thompson, G. A., & Ohlrogge, J. B. (1994) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1994.tb47398.x · Cited by 49 · see citing works
Ferreira, V. D. S., Rocchetta, I., Conforti, V., Bench, S. R., Feldman, R., & Levin, M. J. (2006) · Gene · doi:10.1016/j.gene.2006.10.023 · Cited by 44 · see citing works
Ramos, A. L., Torello, C. O., & Queiroz, M. L. S. (2010) · Nutrition and Cancer · doi:10.1080/01635581.2010.513801 · Cited by 43 · see citing works
Diacylglycerol kinases: at the hub of cell signalling
Mérida, I., Ávila-FLores, A., & Merino, E. (2008) · Biochemical Journal · doi:10.1042/bj4100631v · Cited by 15 · see citing works
Tong, W., Cao, X. M., Harris, S., Sun, H., Fang, H., Fuscoe, J. C., Harris, A. J., Hong, H., Xie, Q., Perkins, R., et al. (2003) · Environmental Health Perspectives · doi:10.1289/txg.6497 · Cited by 13 · see citing works
Post-transcriptional regulation of chloroplast gene expression in Chlamydomonas reinhardtii
Rochaix, J. (1996) · Springer eBooks · doi:10.1007/978-94-009-0353-1_15 · Cited by 12 · see citing works
Dickey, L. A., Teter, B. B., Sampugna, J., & Woods, L. C. (2002) · North American Journal of Aquaculture · doi:10.1577/1548-8454(2002)064 · Cited by 10 · see citing works
Ward, A. J., & Kumar, M. S. (2009) · Bioresource Technology · doi:10.1016/j.biortech.2009.11.053 · Cited by 9 · see citing works
Translational Regulation of Gene Expression in Chloroplasts and Mitochondria
Gillham, N. W. (1994) · Annual Review of Genetics · doi:10.1146/annurev.genet.28.1.71 · Cited by 5 · see citing works
Flagged first, then by citations
Research works printed but not linked: 1
- [47]Ratledge C 1988 An overview of microbial lipids. RCaW SG New York Academic Press
Cited by
Sorted by citations
Breuer, G., Lamers, P. P., Martens, D. E., Draaisma, R. B., & Wijffels, R. H. (2012) · Bioresource Technology · doi:10.1016/j.biortech.2012.08.003 · Cited by 626 · see citing works
Enzymatic cell wall degradation of Chlorella vulgaris and other microalgae for biofuels production
Gerken, H. G., Donohoe, B. S., & Knoshaug, E. P. (2012) · Planta · doi:10.1007/s00425-012-1765-0 · Cited by 454 · see citing works
Food commodities from microalgae
Draaisma, R. B., Wijffels, R. H., (Ellen) Slegers, P., Brentner, L. B., Roy, A., & Barbosa, M. J. (2013) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2012.09.012 · Cited by 432 · see citing works
Talebi, A. F., Mohtashami, S. K., Tabatabaei, M., Tohidfar, M., Bagheri, A., Zeinalabedini, M., Mirzaei, H. H., Mirzajanzadeh, M., Malekzadeh-Shafaroudi, S., & Bakhtiari, S. (2013) · Algal Research · doi:10.1016/j.algal.2013.04.003 · Cited by 390 · see citing works
Kumar, G., Shekh, A., Jakhu, S., Sharma, Y., Kapoor, R., & Sharma, T. R. (2020) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2020.00914 · Cited by 315 · see citing works
Ho, S., Ye, X., Hasunuma, T., Chang, J., & Kondo, A. (2014) · Biotechnology Advances · doi:10.1016/j.biotechadv.2014.09.002 · Cited by 310 · see citing works
Holzinger, A., & Karsten, U. (2013) · Frontiers in Plant Science · doi:10.3389/fpls.2013.00327 · Cited by 307 · see citing works
Advances in the Production of High-Value Products by Microalgae
Leu, S., & Boussiba, S. (2014) · Industrial Biotechnology · doi:10.1089/ind.2013.0039 · Cited by 253 · see citing works
Jia, J., Han, D., Gerken, H. G., Li, Y., Sommerfeld, M. R., Hu, Q., & Xu, J. (2014) · Algal Research · doi:10.1016/j.algal.2014.11.005 · Cited by 239 · see citing works
Edible oils from microalgae: insights in TAG accumulation
Klok, A. J., Lamers, P. P., Martens, D. E., Draaisma, R. B., & Wijffels, R. H. (2014) · Trends in biotechnology · doi:10.1016/j.tibtech.2014.07.004 · Cited by 219 · see citing works
Rismani‐Yazdi, H., HAZNEDAROĞLU, B. Z., Hsin, C., & Peccia, J. (2012) · Biotechnology for Biofuels · doi:10.1186/1754-6834-5-74 · Cited by 213 · see citing works
Gonçalves, E., Wilkie, A. C., Kirst, M., & Rathinasabapathi, B. (2016) · Plant Biotechnology Journal · doi:10.1111/pbi.12523 · Cited by 212 · see citing works
Gwak, Y., Hwang, Y., Wang, B., Kim, M., Jeong, J., Lee, C., Hu, Q., Han, D., & Jin, E. (2014) · Journal of Experimental Botany · doi:10.1093/jxb/eru206 · Cited by 194 · see citing works
Tanaka, T., Maeda, Y., Veluchamy, A., Tanaka, M., Abida, H., Maréchal, É., Bowler, C., Muto, M., Sunaga, Y., Tanaka, M., et al. (2015) · The Plant Cell · doi:10.1105/tpc.114.135194 · Cited by 186 · see citing works
Recent advancements in astaxanthin production from microalgae: A review
Patel, A. K., Tambat, V. S., Chen, C., Chauhan, A. S., Kumar, P., Vadrale, A. P., Huang, C.-Y., Dong, C., & Singhania, R. R. (2022) · Bioresource Technology · doi:10.1016/j.biortech.2022.128030 · Cited by 179 · see citing works
Kang, N. K., Jeon, S., Kwon, S., Koh, H. G., Shin, S.-E., Lee, B., Choi, G., Yang, J.-W., Jeong, B., & Chang, Y. K. (2015) · Biotechnology for Biofuels · doi:10.1186/s13068-015-0386-9 · Cited by 150 · see citing works
PROTEOMICS in aquaculture: Applications and trends
Rodrigues, P. M., Silva, T. S., Dias, J. P., & Jessen, F. (2012) · Journal of Proteomics · doi:10.1016/j.jprot.2012.03.042 · Cited by 152 · see citing works
Wase, N. V., Black, P. N., Stanley, B. A., & Dirusso, C. (2014) · Journal of Proteome Research · doi:10.1021/pr400952z · Cited by 148 · see citing works
A look at diacylglycerol acyltransferases (DGATs) in algae
Chen, J. E., & Smith, A. G. (2012) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2012.05.009 · Cited by 145 · see citing works
Impact of Flue Gas Compounds on Microalgae and Mechanisms for Carbon Assimilation and Utilization
Vuppaladadiyam, A. K., Yao, J. G., Florin, N., George, A., Wang, X., Labeeuw, L., Jiang, Y., Davis, R., Abbas, A., Ralph, P. J., et al. (2017) · ChemSusChem · doi:10.1002/cssc.201701611 · Cited by 141 · see citing works
Omics approaches for microalgal applications: Prospects and challenges
Mishra, A., Medhi, K., Malaviya, P., & Thakur, I. S. (2019) · Bioresource Technology · doi:10.1016/j.biortech.2019.121890 · Cited by 141 · see citing works
Holzinger, A., Kaplan, F., Blaas, K., Zechmann, B., Komsic-Buchmann, K., & Becker, B. (2014) · PLoS ONE · doi:10.1371/journal.pone.0110630 · Cited by 136 · see citing works
Arora, N., Patel, A., Pruthi, P. A., & Pruthi, V. (2016) · Bioresource Technology · doi:10.1016/j.biortech.2016.02.112 · Cited by 135 · see citing works
Ng, I., Keskin, B. B., & Tan, S. (2020) · Biotechnology Journal · doi:10.1002/biot.201900228 · Cited by 135 · see citing works
Engineering fatty acid biosynthesis in microalgae for sustainable biodiesel
Blatti, J. L., Michaud, J. M., & Burkart, M. D. (2013) · Current Opinion in Chemical Biology · doi:10.1016/j.cbpa.2013.04.007 · Cited by 131 · see citing works
Rosenberg, J. N., Kobayashi, N., Barnes, A., Noel, E. A., Betenbaugh, M. J., & Oyler, G. A. (2014) · PLoS ONE · doi:10.1371/journal.pone.0092460 · Cited by 130 · see citing works
Remmers, I. M., D’Adamo, S., Martens, D. E., de Vos, R. C. H., Mumm, R., America, A. H. P., Cordewener, J. H. G., Bakker, L. V., Peters, S., Wijffels, R. H., et al. (2018) · Algal Research · doi:10.1016/j.algal.2018.08.012 · Cited by 129 · see citing works
Longworth, J., Wu, D., Huete-Ortega, M., Wright, P. C., & Vaidyanathan, S. (2016) · Algal Research · doi:10.1016/j.algal.2016.06.015 · Cited by 127 · see citing works
Zúñiga, C., Li, C., Huelsman, T., Levering, J., Zielinski, D. C., McConnell, B. O., Long, C. P., Knoshaug, E. P., Guarnieri, M. T., Antoniewicz, M. R., et al. (2016) · PLANT PHYSIOLOGY · doi:10.1104/pp.16.00593 · Cited by 129 · see citing works
Self-citationRecent developments on biofuels production from microalgae and macroalgae
Kumar, K., Ghosh, S., Angelidaki, İ., Holdt, S. L., Karakashev, D. B., Alvarado-Morales, M., & Das, D. (2016) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2016.06.055 · Cited by 124 · see citing works
Zhang, J., Hao, Q., Bai, L., Xu, J., Yin, W.-B., Song, L., Xu, L., Guo, X., Fan, C., Chen, Y., et al. (2014) · Biotechnology for Biofuels · doi:10.1186/s13068-014-0128-4 · Cited by 122 · see citing works
The effect of nitrogen limitation on the physiology and metabolism of chlorella vulgaris var L3
Ikaran, Z., Suárez-Álvarez, S., Urreta, I., & Castañón, S. (2015) · Algal Research · doi:10.1016/j.algal.2015.04.023 · Cited by 123 · see citing works
Kang, N. K., Kim, E. K., Kim, Y. U., Lee, B., Jeong, W.-J., Jeong, B., & Chang, Y. K. (2017) · Biotechnology for Biofuels · doi:10.1186/s13068-017-0919-5 · Cited by 119 · see citing works
Metabolic engineering of plant oils and waxes for use as industrial feedstocks
Vanhercke, T., Wood, C. C., Stymne, S., Singh, S. P., & Green, A. G. (2012) · Plant Biotechnology Journal · doi:10.1111/pbi.12023 · Cited by 119 · see citing works
Microbial cell immobilization in biohydrogen production: a short overview
Sekoai, P. T., Awosusi, A. A., Yoro, K. O., Singo, M., Oloye, O., Ayeni, A. O., Bodunrin, M. O., & Daramola, M. O. (2017) · Critical Reviews in Biotechnology · doi:10.1080/07388551.2017.1312274 · Cited by 116 · see citing works
Proteomic analysis of Chlorella vulgaris: Potential targets for enhanced lipid accumulation
Guarnieri, M. T., Nag, A., Yang, S., & Pienkos, P. T. (2013) · Journal of Proteomics · doi:10.1016/j.jprot.2013.05.025 · Cited by 113 · see citing works
Self-citationRNA i‐based targeted gene knockdown in the model oleaginous microalgae Nannochloropsis oceanica
Wei, L., Yi, X., Wang, Q., Yang, J., Hu, H., & Xu, J. (2016) · The Plant Journal · doi:10.1111/tpj.13411 · Cited by 108 · see citing works
Characterization of major lipid droplet proteins from Dunaliella
Davidi, L., Katz, A., & Pick, U. (2012) · Planta · doi:10.1007/s00425-011-1585-7 · Cited by 106 · see citing works
Lenka, S. K., Carbonaro, N. J., Park, R., Miller, S. M., Thorpe, I. F., & Li, Y. (2016) · Biotechnology Advances · doi:10.1016/j.biotechadv.2016.06.004 · Cited by 103 · see citing works
Advances and challenges in genetic engineering of microalgae
Fajardo, C., Donato, M. D., Carrasco-Reinado, R., Martı́nez-Rodrı́guez, G., Mancera, J. M., & Fernández‐Acero, F. J. (2019) · Reviews in Aquaculture · doi:10.1111/raq.12322 · Cited by 103 · see citing works
Ezhumalai, G., Arun, M., Manavalan, A., Rajkumar, R., & Heese, K. (2024) · Microbial Ecology · doi:10.1007/s00248-024-02376-1 · Cited by 99 · see citing works
Optimized inorganic carbon regime for enhanced growth and lipid accumulation in Chlorella vulgaris
Lohman, E. J., Gardner, R. D., Pedersen, T. C., Peyton, B., Cooksey, K. E., & Gerlach, R. (2015) · Biotechnology for Biofuels · doi:10.1186/s13068-015-0265-4 · Cited by 99 · see citing works
Leveraging algal omics to reveal potential targets for augmenting TAG accumulation
Arora, N., Pienkos, P. T., Pruthi, V., Poluri, K. M., & Guarnieri, M. T. (2018) · Biotechnology Advances · doi:10.1016/j.biotechadv.2018.04.005 · Cited by 98 · see citing works
Self-citationAlgal omics: unlocking bioproduct diversity in algae cell factories
Guarnieri, M. T., & Pienkos, P. T. (2015) · Photosynthesis Research · doi:10.1007/s11120-014-9989-4 · Cited by 97 · see citing works
Self-citationPerera, I. A., Abinandan, S., Subashchandrabose, S. R., Venkateswarlu, K., Naidu, R., & Megharaj, M. (2019) · Critical Reviews in Biotechnology · doi:10.1080/07388551.2019.1597828 · Cited by 96 · see citing works
Nagappan, S., Devendran, S., Tsai, P., Jayaraman, H., Alagarsamy, V., Pugazhendhi, A., & Ponnusamy, V. K. (2019) · Process Biochemistry · doi:10.1016/j.procbio.2019.11.027 · Cited by 95 · see citing works
Yang, B., Liu, J., Jiang, Y., & Chen, F. (2016) · Biotechnology Journal · doi:10.1002/biot.201500617 · Cited by 94 · see citing works
Menon, K. R., Balan, R., & Suraishkumar, G. K. (2013) · Biotechnology and Bioengineering · doi:10.1002/bit.24835 · Cited by 91 · see citing works
Pichrtová, M., Kulichová, J., & Holzinger, A. (2014) · PLoS ONE · doi:10.1371/journal.pone.0113137 · Cited by 89 · see citing works
Ten years of algal biofuel and bioproducts: gains and pains
Chen, H., Li, T., & Wang, Q. (2019) · Planta · doi:10.1007/s00425-018-3066-8 · Cited by 83 · see citing works
Rai, V., Muthuraj, M., Gandhi, M., Das, D., & Srivastava, S. (2017) · Scientific Reports · doi:10.1038/srep45732 · Cited by 82 · see citing works
Hanifzadeh, M., Cerdán-García, E., & Viamajala, S. (2018) · Renewable Energy · doi:10.1016/j.renene.2018.05.012 · Cited by 79 · see citing works
Microalgae, Functional Genomics and Biotechnology
Cadoret, J., Garnier, M., & Saint‐Jean, B. (2012) · Advances in botanical research · doi:10.1016/b978-0-12-391499-6.00008-6 · Cited by 79 · see citing works
Dall’Osto, L., Cazzaniga, S., Guardini, Z., Barera, S., Benedetti, M., Mannino, G., Maffei, M. E., & Bassi, R. (2019) · Biotechnology for Biofuels · doi:10.1186/s13068-019-1566-9 · Cited by 78 · see citing works
Liu, T., Li, Y., Liu, F., & Wang, C. (2016) · Bioresource Technology · doi:10.1016/j.biortech.2015.12.021 · Cited by 72 · see citing works
Ndimba, B. K., Ndimba, R. J., Johnson, T. S., Waditee‐Sirisattha, R., Baba, M., Sirisattha, S., Shiraiwa, Y., Agrawal, G. K., & Rakwal, R. (2013) · Journal of Proteomics · doi:10.1016/j.jprot.2013.05.041 · Cited by 72 · see citing works
Garnier, M., Carrier, G., Rogniaux, H., Nicolau, É., Bougaran, G., Saint‐Jean, B., & Cadoret, J. (2014) · Journal of Proteomics · doi:10.1016/j.jprot.2014.02.022 · Cited by 70 · see citing works
Microalgae Biomass and Lipids as Feedstock for Biofuels: Sustainable Biotechnology Strategies
Babu, S. S., Gondi, R., Vincent, G. S., JohnSamuel, G. C., & Jeyakumar, R. B. (2022) · Sustainability · doi:10.3390/su142215070 · Cited by 67 · see citing works
Gonçalves, E., Johnson, J. V., & Rathinasabapathi, B. (2013) · Planta · doi:10.1007/s00425-013-1946-5 · Cited by 65 · see citing works
Predicting Dynamic Metabolic Demands in the Photosynthetic Eukaryote Chlorella vulgaris
Zúñiga, C., Levering, J., Antoniewicz, M. R., Guarnieri, M. T., Betenbaugh, M. J., & Zengler, K. (2017) · PLANT PHYSIOLOGY · doi:10.1104/pp.17.00605 · Cited by 66 · see citing works
Self-citationLiu, J., Sun, Z., Zhong, Y., Huang, J., Hu, Q., & Chen, F. (2012) · Planta · doi:10.1007/s00425-012-1718-7 · Cited by 63 · see citing works
Shaikh, K. M., Nesamma, A. A., Abdin, M. Z., & Jutur, P. P. (2019) · Biotechnology for Biofuels · doi:10.1186/s13068-019-1521-9 · Cited by 62 · see citing works
Molnár, I., Lopez, D., Wisecaver, J. H., Devarenne, T. P., Weiss, T. L., Pellegrini, M. A., & Hackett, J. D. (2012) · BMC Genomics · doi:10.1186/1471-2164-13-576 · Cited by 61 · see citing works
Can Omics Approaches Improve Microalgal Biofuels under Abiotic Stress?
Salama, E., Govindwar, S. P., Khandare, R. V., Roh, H., Jeon, B., & Li, X. (2019) · Trends in Plant Science · doi:10.1016/j.tplants.2019.04.001 · Cited by 62 · see citing works
The state of algal genome quality and diversity
Hanschen, E. R., & Starkenburg, S. R. (2020) · Algal Research · doi:10.1016/j.algal.2020.101968 · Cited by 61 · see citing works
Genetic Engineering Strategies for Enhanced Biodiesel Production
Hegde, K., Chandra, N., Sarma, S. J., Brar, S. K., & Dasu, V. V. (2015) · Molecular Biotechnology · doi:10.1007/s12033-015-9869-y · Cited by 60 · see citing works
Gonçalves, E., Koh, J., Zhu, N., Yoo, M., Chen, S., Matsuo, T., Johnson, J. V., & Rathinasabapathi, B. (2016) · The Plant Journal · doi:10.1111/tpj.13144 · Cited by 58 · see citing works
Metabolic pathways for lipid synthesis under nitrogen stress in Chlamydomonas and Nannochloropsis
Banerjee, A., Maiti, S. K., Guria, C., & Banerjee, C. (2016) · Biotechnology Letters · doi:10.1007/s10529-016-2216-y · Cited by 58 · see citing works
Environmental Risks of Large Scale Cultivation of Microalgae: Mitigation of Spills
Gressel, J., van der Vlugt, C. J. B., & Bergmans, H. E. N. (2013) · Algal Research · doi:10.1016/j.algal.2013.04.002 · Cited by 55 · see citing works
Allen, J. W., Dirusso, C., & Black, P. N. (2015) · Algal Research · doi:10.1016/j.algal.2015.04.019 · Cited by 55 · see citing works
Identification and Metabolite Profiling of Chemical Activators of Lipid Accumulation in Green Algae
Wase, N. V., Tu, B., Allen, J. W., Black, P. N., & Dirusso, C. (2017) · PLANT PHYSIOLOGY · doi:10.1104/pp.17.00433 · Cited by 55 · see citing works
Xing, G., Yuan, H., Yang, J., Li, J., Gao, Q., Li, W., & Wang, E. T. (2018) · Algal Research · doi:10.1016/j.algal.2018.04.028 · Cited by 55 · see citing works
Zhao, Y., Hou, Y., Chai, W., Liu, Z., Wang, X., He, C., Hu, Z., Chen, S., Wang, W., & chen, F. (2019) · Enzyme and Microbial Technology · doi:10.1016/j.enzmictec.2019.109487 · Cited by 56 · see citing works
Tran, N.-A. T., Padula, M. P., Evenhuis, C. R., Commault, A. S., Ralph, P. J., & Tamburic, B. (2016) · Algal Research · doi:10.1016/j.algal.2016.07.009 · Cited by 53 · see citing works
Kudahettige, N. P., Picková, J., & Gentili, F. G. (2018) · Frontiers in Energy Research · doi:10.3389/fenrg.2018.00132 · Cited by 53 · see citing works
Kuo, E. Y., Yang, R., Chin, Y. Y., Chien, Y., Chen, Y. C., Wei, C., Kao, L., Chang, Y., Li, Y., Chen, T., et al. (2022) · Biotechnology Journal · doi:10.1002/biot.202100603 · Cited by 51 · see citing works
Traller, J. C., & Hildebrand, M. (2013) · Algal Research · doi:10.1016/j.algal.2013.03.003 · Cited by 51 · see citing works
Biotechnological and bioinformatics approaches for augmentation of biohydrogen production: A review
Kumar, G. R., & Chowdhary, N. (2015) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2015.12.022 · Cited by 51 · see citing works
Rincón, S. M., Urrego, N. F., Avila, K., Romero, H. M., & Beyenal, H. (2019) · Algal Research · doi:10.1016/j.algal.2019.101408 · Cited by 51 · see citing works
Li, L., Zhang, G., & Wang, Q. (2016) · BMC Microbiology · doi:10.1186/s12866-016-0839-8 · Cited by 50 · see citing works
Sekoai, P. T., & Daramola, M. O. (2018) · Chemical Engineering Communications · doi:10.1080/00986445.2018.1428958 · Cited by 50 · see citing works
Cheng, J., Niu, Y., Lu, S., & Yuan, Y. (2012) · Journal of Chemical Technology & Biotechnology · doi:10.1002/jctb.3759 · Cited by 49 · see citing works
Lipid Metabolism in Microalgae
Khozin‐Goldberg, I. (2016) · Springer eBooks · doi:10.1007/978-3-319-24945-2_18 · Cited by 47 · see citing works
Tripathi, S., Kairamkonda, M., Gupta, P., & Poluri, K. M. (2022) · Chemical Engineering Journal · doi:10.1016/j.cej.2022.140068 · Cited by 44 · see citing works
Misra, N., Panda, P. K., & Parida, B. K. (2013) · OMICS A Journal of Integrative Biology · doi:10.1089/omi.2013.0025 · Cited by 46 · see citing works
Proteome response of Dunaliella parva induced by nitrogen limitation
Shang, C., Zhu, S., Wang, Z., Qin, L., Alam, M. A., Xie, J., & Yuan, Z. (2017) · Algal Research · doi:10.1016/j.algal.2017.01.016 · Cited by 45 · see citing works
Kumar, R., Biswas, K., Singh, P. K., Singh, P. K., Sanniyasi, E., Shukla, P., & Pabbi, S. (2017) · Biotechnology for Biofuels · doi:10.1186/s13068-017-0776-2 · Cited by 44 · see citing works
Lim, D. K. Y., Schuhmann, H., Thomas‐Hall, S. R., Chan, C. K., Wass, T. J., Aguilera, F., Adarme-Vega, T. C., Dal’Molin, C. G. D. O., Thorpe, G. J., Batley, J., et al. (2017) · Bioresource Technology · doi:10.1016/j.biortech.2017.06.003 · Cited by 45 · see citing works
Sharma, T., & Chauhan, R. S. (2016) · Algal Research · doi:10.1016/j.algal.2016.07.020 · Cited by 45 · see citing works
Yang, S., Guarnieri, M. T., Smolinski, S. L., Ghirardi, M. L., & Pienkos, P. T. (2013) · Biotechnology for Biofuels · doi:10.1186/1754-6834-6-118 · Cited by 43 · see citing works
Self-citationArora, N., Laurens, L. M. L., Sweeney, N., Pruthi, V., Poluri, K. M., & Pienkos, P. T. (2018) · Algal Research · doi:10.1016/j.algal.2018.12.003 · Cited by 43 · see citing works
Self-citationMorales‐Sánchez, D., Tinoco‐Valencia, R., Caro-Bermúdez, M. A., & Martı́nez, A. (2014) · Algal Research · doi:10.1016/j.algal.2014.05.006 · Cited by 42 · see citing works
Hua, Y., Wang, S., Liu, Z., Liu, X., Zou, L., Gu, W., Hou, Y., Ma, Y., Yiyuan, L., & Liu, J. (2016) · Journal of Proteomics · doi:10.1016/j.jprot.2016.02.027 · Cited by 42 · see citing works
Rai, V., Karthikaichamy, A., Das, D., Noronha, S. B., Wangikar, P. P., & Srivastava, S. (2016) · OMICS A Journal of Integrative Biology · doi:10.1089/omi.2016.0065 · Cited by 38 · see citing works
Challenges and Opportunities in the Present Era of Marine Algal Applications
Beetul, K., Gopeechund, A., Kaullysing, D., Mattan-Moorgawa, S., Puchooa, D., & Bhagooli, R. (2016) · InTech eBooks · doi:10.5772/63272 · Cited by 38 · see citing works
Li, C., Yelsky, J. S., Chen, Y., Zúñiga, C., Eng, R., Jiang, L., Shapiro, A. J., Huang, K.-W., Zengler, K., & Betenbaugh, M. J. (2019) · npj Systems Biology and Applications · doi:10.1038/s41540-019-0110-7 · Cited by 40 · see citing works
Azaman, S. N. A., Wong, D. C. J., Tan, S. W., Yusoff, F. M., Nagao, N., & Yeap, S. K. (2020) · Scientific Reports · doi:10.1038/s41598-020-74410-4 · Cited by 38 · see citing works
Srirangan, S., Sauer, M.-L., Howard, B. E., Dvora, M., Dums, J. T., Backman, P., & Sederoff, H. W. (2015) · PLoS ONE · doi:10.1371/journal.pone.0127562 · Cited by 38 · see citing works
Poong, S., Lim, P., Phang, S., Wong, C.-Y., Pai, T., Chen, C., Yang, C.-H., & Liu, C.-C. (2017) · Journal of Applied Phycology · doi:10.1007/s10811-017-1124-4 · Cited by 37 · see citing works
Algal Biofuels: An Economic and Effective Alternative of Fossil Fuels
Bhardwaj, N., Agrawal, K., & Verma, P. (2020) · Clean Energy Production Technologies · doi:10.1007/978-981-15-7190-9_7 · Cited by 36 · see citing works
- Authors
- Guarnieri, M. T., Nag, A., Smolinski, S. L., Darzins, A., Seibert, M., & Pienkos, P. T.
- Affiliations
- National Laboratory of the Rockies (US)
- Corresponding author
- Guarnieri, Michael T.
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2011-10-17
- Volume
- 6
- Issue
- 10
- Pages
- e25851
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC3197185
- OpenAlex
- W2028421881
- Cited by
- 234
- References
- 55
- Citations by year
- 2026: 6 · 2025: 11 · 2024: 5 · 2023: 1 · 2022: 11 · 2021: 9 · 2020: 17 · 2019: 24 · 2018: 16 · 2017: 18 · 2016: 34 · 2015: 19 · 2014: 23 · 2013: 23 · 2012: 17
- FWCI
- 8.37
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- PLoS ONE (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- U.S. Department of Energy (DE -AC36-08GO28308, No. DE-AC36-08GO28308, 08GO28308, Contract No. DE-AC36-08GO28308, AC36‐08GO28308)
- University of Nebraska-Lincoln
- Office of Energy Efficiency and Renewable Energy (Contract No. DE-AC36-08GO28308, DE-AC36-08-GO28308., AC36-08-GO28308, No. DE-AC36-08GO28308)
- Laboratory Directed Research and Development (DE-AC36-08GO28308)
- Office of Energy Efficiency
- National Renewable Energy Laboratory (Contract No. DE-AC36-08GO28308, DE-AC36-08-GO28308, AC36-08GO28308)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- triglyceride synthesis, oleaginous microalgae, Chlorella vulgaris, de novo transcriptome assembly, fatty acid synthesis, lipid accumulation, algal biofuels
- MeSH terms
- Oils, Triglycerides, Up-Regulation, Gene Expression Profiling, Proteomics, Biosynthetic Pathways, Biofuels, Microalgae
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
