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Cited by 51Genomics and Chromatin DynamicsThyroid Disorders and TreatmentsThyroid Cancer Diagnosis and Treatment

Genome-wide analysis of Pax8 binding provides new insights into thyroid functions

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Ruiz-Llorente, Sergio, de Pau, Enrique Carrillo Santa, Sastre-Perona, Ana, Montero-Conde, Cristina, Gómez-López, Gonzalo, Fagin, James A, Valencia, Alfonso, Pisano, David G +1 more·2012·BMC Genomics
DOI 10.1186/1471-2164-13-147PMC PMC3403905
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Abstract

Background: The transcription factor Pax8 is essential for the differentiation of thyroid cells. However, there are few data on genes transcriptionally regulated by Pax8 other than thyroid-related genes. To better understand the role of Pax8 in the biology of thyroid cells, we obtained transcriptional profiles of Pax8-silenced PCCl3 thyroid cells using whole genome expression arrays and integrated these signals with global cis-regulatory sequencing studies performed by ChIP-Seq analysis Results: Exhaustive analysis of Pax8 immunoprecipitated peaks demonstrated preferential binding to intragenic regions and CpG-enriched islands, which suggests a role of Pax8 in transcriptional regulation of orphan CpG regions. In addition, ChIP-Seq allowed us to identify Pax8 partners, including proteins involved in tertiary DNA structure (CTCF) and chromatin remodeling (Sp1), and these direct transcriptional interactions were confirmed in vivo . Moreover, both factors modulate Pax8-dependent transcriptional activation of the sodium iodide symporter ( Nis ) gene promoter. We ultimately combined putative and novel Pax8 binding sites with actual target gene expression regulation to define Pax8-dependent genes. Functional classification suggests that Pax8-regulated genes may be directly involved in important processes of thyroid cell function such as cell proliferation and differentiation, apoptosis, cell polarity, motion and adhesion, and a plethora of DNA/protein-related processes. Conclusion: Our study provides novel insights into the role of Pax8 in thyroid biology, exerted through transcriptional regulation of important genes involved in critical thyrocyte processes. In addition, we found new transcriptional partners of Pax8, which functionally cooperate with Pax8 in the regulation of thyroid gene transcription. Besides, our data demonstrate preferential location of Pax8 in non-promoter CpG regions. These data point to an orphan CpG island-mediated mechanism that represents a novel role of Pax8 in the transcriptional output of the thyrocyte.

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  • Abolishing PAX8 downregulates DIO1 expression in thyroid cells, suggesting PAX8 activates DIO1 transcriptionally, likely mediated by the selenocysteine insertion sequence in the 3' UTR of DIO1.

    “Abolishing PAX8 downregulated DIO1 expression in thyroid cells, suggesting that PAX8 activates DIO1 transcriptionally, which is likely mediated by the selenocystein insertion sequence in the 3’ untranslated region of the DIO1 gene (178).”

    From Intrathyroidal feedforward and feedback network regulating thyroid hormone synthesis and secretion (2022) · cites this paper as [178]

    The Discussion ('Identification of biological processes controlled by Pax8 in thyroid cells'; page unavailable) reports DIO1 downregulation after Pax8 abolition and potential Pax8 binding near a region important for selenocysteine insertion. The Results describe Dio1 among validated targets with reduced expression after Pax8 silencing. But the paper does not establish that transcriptional activation is mediated by a sequence in the DIO1 3′ UTR; the Discussion instead cites preliminary evidence for Pax8-dependent DIO1 mRNA stabilization. (LLM, conf=0.88)

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Research works printed but not linked: 75

  1. Farnham PJ Insights from genomic profiling of transcription factors Nat Rev Genet 2009 10 9 605 616 10.1038/nrg2636 19668247 PMC2846386 · DOI · Paper page
  2. Santisteban P Bernal J Thyroid development and effect on the nervous system Rev Endocr Metab Disord 2005 6 3 217 228 10.1007/s11154-005-3053-9 16151626 · DOI
  3. De Felice M Di Lauro R Thyroid development and its disorders: genetics and molecular mechanisms Endocr Rev 2004 25 5 722 746 10.1210/er.2003-0028 15466939 · DOI
  4. Plachov D Chowdhury K Walther C Simon D Guenet JL Gruss P Pax8, a murine paired box gene expressed in the developing excretory system and thyroid gland Development 1990 110 2 643 651 1723950
  5. Mansouri A Chowdhury K Gruss P Follicular cells of the thyroid gland require Pax8 gene function Nat Genet 1998 19 1 87 90 10.1038/ng0598-87 9590297 · DOI
  6. Macchia PE Lapi P Krude H Pirro MT Missero C Chiovato L Souabni A Baserga M Tassi V Pinchera A PAX8 mutations associated with congenital hypothyroidism caused by thyroid dysgenesis Nat Genet 1998 19 1 83 86 10.1038/ng0598-83 9590296 · DOI
  7. Ohno M Zannini M Levy O Carrasco N di Lauro R The paired-domain transcription factor Pax8 binds to the upstream enhancer of the rat sodium/iodide symporter gene and participates in both thyroid-specific and cyclic-AMP-dependent transcription Mol Cell Biol 1999 19 3 2051 2060 10022892 PMC83998
  8. Miccadei S De Leo R Zammarchi E Natali PG Civitareale D The synergistic activity of thyroid transcription factor 1 and Pax 8 relies on the promoter/enhancer interplay Mol Endocrinol 2002 16 4 837 846 10.1210/me.16.4.837 11923479 · DOI
  9. Zannini M Francis-Lang H Plachov D Di Lauro R Pax-8, a paired domain-containing protein, binds to a sequence overlapping the recognition site of a homeodomain and activates transcription from two thyroid-specific promoters Mol Cell Biol 1992 12 9 4230 4241 1508216 PMC360331
  10. Fraizer GC Shimamura R Zhang X Saunders GF PAX 8 regulates human WT1 transcription through a novel DNA binding site J Biol Chem 1997 272 49 30678 30687 10.1074/jbc.272.49.30678 9388203 · DOI
  11. Deaton AM Bird A CpG islands and the regulation of transcription Genes Dev 2011 25 10 1010 1022 10.1101/gad.2037511 21576262 PMC3093116 · DOI · Paper page
  12. Esposito C Miccadei S Saiardi A Civitareale D PAX 8 activates the enhancer of the human thyroperoxidase gene Biochem J 1998 331 Pt 1 37 40 9512459 PMC1219318
  13. Pellizzari L Fabbro D Lonigro R Di Lauro R Damante G A network of specific minor-groove contacts is a common characteristic of paired-domain-DNA interactions Biochem J 1996 315 Pt 2 363 367 8615801 PMC1217204
  14. Czerny T Schaffner G Busslinger M DNA sequence recognition by Pax proteins: bipartite structure of the paired domain and its binding site Genes Dev 1993 7 10 2048 2061 10.1101/gad.7.10.2048 8406007 · DOI
  15. Ho Sui SJ Mortimer JR Arenillas DJ Brumm J Walsh CJ Kennedy BP Wasserman WW oPOSSUM: identification of over-represented transcription factor binding sites in co-expressed genes Nucleic Acids Res 2005 33 10 3154 3164 10.1093/nar/gki624 15933209 PMC1142402 · DOI
  16. Planque N Leconte L Coquelle FM Benkhelifa S Martin P Felder-Schmittbuhl MP Saule S Interaction of Maf transcription factors with Pax-6 results in synergistic activation of the glucagon promoter J Biol Chem 2001 276 38 35751 35760 10.1074/jbc.M104523200 11457839 · DOI
  17. Garcia B Santisteban P PI3K is involved in the IGF-I inhibition of TSH-induced sodium/iodide symporter gene expression Mol Endocrinol 2002 16 2 342 352 10.1210/me.16.2.342 11818505 · DOI
  18. Shih HA Nathanson KL Seal S Collins N Stratton MR Rebbeck TR Weber BL BRCA1 and BRCA2 mutations in breast cancer families with multiple primary cancers Clin Cancer Res 2000 6 11 4259 4264 11106241
  19. Thedieck C Kuczyk M Klingel K Steiert I Muller CA Klein G Expression of Ksp-cadherin during kidney development and in renal cell carcinoma Br J Cancer 2005 92 11 2010 2017 10.1038/sj.bjc.6602597 15886705 PMC2361784 · DOI
  20. Di Palma T Conti A de Cristofaro T Scala S Nitsch L Zannini M Identification of novel Pax8 targets in FRTL-5 thyroid cells by gene silencing and expression microarray analysis PLoS One 2011 6 9 e25162 10.1371/journal.pone.0025162 21966443 PMC3179481 · DOI
  21. de Cristofaro T Di Palma T Fichera I Lucci V Parrillo L De Felice M Zannini M An essential role for Pax8 in the transcriptional regulation of Cadherin-16 in thyroid cells Mol Endocrinol 2012 26 1 67 78 10.1210/me.2011-1090 22135066 PMC5417163 · DOI
  22. Zacchi P Stenmark H Parton RG Orioli D Lim F Giner A Mellman I Zerial M Murphy C Rab17 regulates membrane trafficking through apical recycling endosomes in polarized epithelial cells J Cell Biol 1998 140 5 1039 1053 10.1083/jcb.140.5.1039 9490718 PMC2132691 · DOI
  23. Muller T Hess MW Schiefermeier N Pfaller K Ebner HL Heinz-Erian P Ponstingl H Partsch J Rollinghoff B Kohler H MYO5B mutations cause microvillus inclusion disease and disrupt epithelial cell polarity Nat Genet 2008 40 10 1163 1165 10.1038/ng.225 18724368 · DOI
  24. Min J Zaslavsky A Fedele G McLaughlin SK Reczek EE De Raedt T Guney I Strochlic DE Macconaill LE Beroukhim R An oncogene-tumor suppressor cascade drives metastatic prostate cancer by coordinately activating Ras and nuclear factor-kappaB Nat Med 2010 16 3 286 294 10.1038/nm.2100 20154697 PMC2903662 · DOI
  25. Nowak RB Fischer RS Zoltoski RK Kuszak JR Fowler VM Tropomodulin1 is required for membrane skeleton organization and hexagonal geometry of fiber cells in the mouse lens J Cell Biol 2009 186 6 915 928 10.1083/jcb.200905065 19752024 PMC2753162 · DOI
  26. Liu C Papewalis C Domberg J Scherbaum WA Schott M Chemokines and autoimmune thyroid diseases Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme 2008 40 6 361 368 10.1055/s-2008-1073153 18418812 · DOI
  27. Min HS Choe G Kim SW Park YJ Park do J Youn YK Park SH Cho BY Park SY S100A4 expression is associated with lymph node metastasis in papillary microcarcinoma of the thyroid Modern pathology: an official journal of the United States and Canadian Academy of Pathology, Inc 2008 21 6 748 755 18360353
  28. Ying S Dong S Kawada A Kojima T Chavanas S Mechin MC Adoue V Serre G Simon M Takahara H Transcriptional regulation of peptidylarginine deiminase expression in human keratinocytes J Dermatol Sci 2009 53 1 2 9 10.1016/j.jdermsci.2008.09.009 19004619 · DOI
  29. Riepe FG Clinical and molecular features of type 1 pseudohypoaldosteronism Horm Res 2009 72 1 1 9 19571553
  30. Montero-Conde C Martin-Campos JM Lerma E Gimenez G Martinez-Guitarte JL Combalia N Montaner D Matias-Guiu X Dopazo J de Leiva A Molecular profiling related to poor prognosis in thyroid carcinoma. Combining gene expression data and biological information Oncogene 2008 27 11 1554 1561 10.1038/sj.onc.1210792 17873908 · DOI
  31. Stuart ET Haffner R Oren M Gruss P Loss of p53 function through PAX-mediated transcriptional repression EMBO J 1995 14 22 5638 5645 8521821 PMC394679
  32. Dehbi M Pelletier J PAX8-mediated activation of the wt1 tumor suppressor gene EMBO J 1996 15 16 4297 4306 8861958 PMC452155
  33. Scouten WT Patel A Terrell R Burch HB Bernet VJ Tuttle RM Francis GL Cytoplasmic localization of the paired box gene, Pax-8, is found in pediatric thyroid cancer and may be associated with a greater risk of recurrence Thyroid 2004 14 12 1037 1046 10.1089/thy.2004.14.1037 15650356 · DOI
  34. Scacheri PC Davis S Odom DT Crawford GE Perkins S Halawi MJ Agarwal SK Marx SJ Spiegel AM Meltzer PS Genome-wide analysis of menin binding provides insights into MEN1 tumorigenesis PLoS Genet 2006 2 4 e51 10.1371/journal.pgen.0020051 16604156 PMC1428788 · DOI
  35. Yang A Zhu Z Kapranov P McKeon F Church GM Gingeras TR Struhl K Relationships between p63 binding, DNA sequence, transcription activity, and biological function in human cells Mol Cell 2006 24 4 593 602 10.1016/j.molcel.2006.10.018 17188034 · DOI
  36. A M A AP AS M M B Z M M H Evaluation of apoptosis-related genes; Fas (CD94), FasL (CD178) and TRAIL polymorphisms in Iranian multiple sclerosis patients J Neurol Sci 2011 21864851
  37. Illingworth RS Bird AP CpG islands–'a rough guide' FEBS Lett 2009 583 11 1713 1720 10.1016/j.febslet.2009.04.012 19376112 · DOI
  38. Illingworth RS Gruenewald-Schneider U Webb S Kerr AR James KD Turner DJ Smith C Harrison DJ Andrews R Bird AP Orphan CpG islands identify numerous conserved promoters in the mammalian genome PLoS Genet 2010 6 9 PMC2944787 20885785
  39. Kimura K Wakamatsu A Suzuki Y Ota T Nishikawa T Yamashita R Yamamoto J Sekine M Tsuritani K Wakaguri H Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes Genome Res 2006 16 1 55 65 16344560 PMC1356129
  40. Guttman M Amit I Garber M French C Lin MF Feldser D Huarte M Zuk O Carey BW Cassady JP Chromatin signature reveals over a thousand highly conserved large non-coding RNAs in mammals Nature 2009 458 7235 223 227 10.1038/nature07672 19182780 PMC2754849 · DOI · Paper page
  41. Maunakea AK Nagarajan RP Bilenky M Ballinger TJ D'Souza C Fouse SD Johnson BE Hong C Nielsen C Zhao Y Conserved role of intragenic DNA methylation in regulating alternative promoters Nature 2010 466 7303 253 257 10.1038/nature09165 20613842 PMC3998662 · DOI · Paper page
  42. Ohler U Liao GC Niemann H Rubin GM Computational analysis of core promoters in the Drosophila genome Genome Biol 2002 3 12 RESEARCH0087 PMC151189 12537576
  43. Lim CY Santoso B Boulay T Dong E Ohler U Kadonaga JT The MTE, a new core promoter element for transcription by RNA polymerase II Genes Dev 2004 18 13 1606 1617 10.1101/gad.1193404 15231738 PMC443522 · DOI
  44. Juven-Gershon T Hsu JY Theisen JW Kadonaga JT The RNA polymerase II core promoter - the gateway to transcription Curr Opin Cell Biol 2008 20 3 253 259 10.1016/j.ceb.2008.03.003 18436437 PMC2586601 · DOI · Paper page
  45. Jin VX Singer GA Agosto-Perez FJ Liyanarachchi S Davuluri RV Genome-wide analysis of core promoter elements from conserved human and mouse orthologous pairs BMC Bioinforma 2006 7 114 10.1186/1471-2105-7-114 PMC1475891 16522199 · DOI
  46. Di Palma T D'Andrea B Liguori GL Liguoro A de Cristofaro T Del Prete D Pappalardo A Mascia A Zannini M TAZ is a coactivator for Pax8 and TTF-1, two transcription factors involved in thyroid differentiation Exp Cell Res 2009 315 2 162 175 10.1016/j.yexcr.2008.10.016 19010321 · DOI
  47. Lanctot C Cheutin T Cremer M Cavalli G Cremer T Dynamic genome architecture in the nuclear space: regulation of gene expression in three dimensions Nat Rev Genet 2007 8 2 104 115 10.1038/nrg2041 17230197 · DOI · Paper page
  48. Phillips JE Corces VG CTCF: master weaver of the genome Cell 2009 137 7 1194 1211 10.1016/j.cell.2009.06.001 19563753 PMC3040116 · DOI
  49. Li L Davie JR The role of Sp1 and Sp3 in normal and cancer cell biology Ann Anat 2010 192 5 275 283 10.1016/j.aanat.2010.07.010 20810260 · DOI
  50. De Leo R Miccadei S Zammarchi E Civitareale D Role for p300 in Pax 8 induction of thyroperoxidase gene expression J Biol Chem 2000 275 44 34100 34105 10924503
  51. Grasberger H Ringkananont U Lefrancois P Abramowicz M Vassart G Refetoff S Thyroid transcription factor 1 rescues PAX8/p300 synergism impaired by a natural PAX8 paired domain mutation with dominant negative activity Mol Endocrinol 2005 19 7 1779 1791 10.1210/me.2004-0426 15718293 · DOI
  52. Pasca di Magliano M Di Lauro R Zannini M Pax8 has a key role in thyroid cell differentiation Proc Natl Acad Sci U S A 2000 97 24 13144 13149 10.1073/pnas.240336397 11069301 PMC27192 · DOI
  53. Robson EJ He SJ Eccles MR A PANorama of PAX genes in cancer and development Nat Rev Cancer 2006 6 1 52 62 10.1038/nrc1778 16397527 · DOI
  54. Arnaldi LA Borra RC Maciel RM Cerutti JM Gene expression profiles reveal that DCN, DIO1, and DIO2 are underexpressed in benign and malignant thyroid tumors Thyroid 2005 15 3 210 221 10.1089/thy.2005.15.210 15785240 · DOI
  55. Rossi DL Acebron A Santisteban P Function of the homeo and paired domain proteins TTF-1 and Pax-8 in thyroid cell proliferation J Biol Chem 1995 270 39 23139 23142 10.1074/jbc.270.39.23139 7559458 · DOI
  56. Buttiglieri S Deregibus MC Bravo S Cassoni P Chiarle R Bussolati B Camussi G Role of Pax2 in apoptosis resistance and proinvasive phenotype of Kaposi's sarcoma cells J Biol Chem 2004 279 6 4136 4143 14627715
  57. Schebesta A McManus S Salvagiotto G Delogu A Busslinger GA Busslinger M Transcription factor Pax5 activates the chromatin of key genes involved in B cell signaling, adhesion, migration, and immune function Immunity 2007 27 1 49 63 10.1016/j.immuni.2007.05.019 17658281 · DOI
  58. Albelda SM Oliver PD Romer LH Buck CA EndoCAM: a novel endothelial cell-cell adhesion molecule J Cell Biol 1990 110 4 1227 1237 10.1083/jcb.110.4.1227 2182647 PMC2116064 · DOI
  59. Cali G Gentile F Mogavero S Pallante P Nitsch R Ciancia G Ferraro A Fusco A Nitsch L CDH16/Ksp-Cadherin Is Expressed in the Developing Thyroid Gland and Is Strongly Down-Regulated in Thyroid Carcinomas Endocrinology 2011 22028439
  60. de Cristofaro T Di Palma T Fichera I Lucci V Parrillo L De Felice M Zannini M An Essential Role for Pax8 in the Transcriptional Regulation of Cadherin-16 in Thyroid Cells Mol Endocrinol 2011 PMC5417163 22135066
  61. Fusco A Berlingieri MT Di Fiore PP Portella G Grieco M Vecchio G One- and two-step transformations of rat thyroid epithelial cells by retroviral oncogenes Mol Cell Biol 1987 7 9 3365 3370 3670314 PMC367981
  62. Costamagna E Garcia B Santisteban P The functional interaction between the paired domain transcription factor Pax8 and Smad3 is involved in transforming growth factor-beta repression of the sodium/iodide symporter gene J Biol Chem 2004 279 5 3439 3446 14623893
  63. Landa I Ruiz-Llorente S Montero-Conde C Inglada-Perez L Schiavi F Leskela S Pita G Milne R Maravall J Ramos I The variant rs1867277 in FOXE1 gene confers thyroid cancer susceptibility through the recruitment of USF1/USF2 transcription factors PLoS genetics 2009 5 9 e1000637 10.1371/journal.pgen.1000637 19730683 PMC2727793 · DOI
  64. Vilain C Rydlewski C Duprez L Heinrichs C Abramowicz M Malvaux P Renneboog B Parma J Costagliola S Vassart G Autosomal dominant transmission of congenital thyroid hypoplasia due to loss-of-function mutation of PAX8 J Clin Endocrinol Metab 2001 86 1 234 238 10.1210/jc.86.1.234 11232006 · DOI
  65. Courey AJ Holtzman DA Jackson SP Tjian R Synergistic activation by the glutamine-rich domains of human transcription factor Sp1 Cell 1989 59 5 827 836 10.1016/0092-8674(89)90606-5 2512012 · DOI
  66. Klenova EM Nicolas RH Paterson HF Carne AF Heath CM Goodwin GH Neiman PE Lobanenkov VV CTCF, a conserved nuclear factor required for optimal transcriptional activity of the chicken c-myc gene, is an 11-Zn-finger protein differentially expressed in multiple forms Mol Cell Biol 1993 13 12 7612 7624 8246978 PMC364833
  67. Lee TI Johnstone SE Young RA Chromatin immunoprecipitation and microarray-based analysis of protein location Nat Protoc 2006 1 2 729 748 10.1038/nprot.2006.98 17406303 PMC3004291 · DOI
  68. Salmon-Divon M Dvinge H Tammoja K Bertone P PeakAnalyzer: genome-wide annotation of chromatin binding and modification loci BMC Bioinforma 2010 11 415 10.1186/1471-2105-11-415 PMC2923140 20691053 · DOI
  69. Flicek P Amode MR Barrell D Beal K Brent S Chen Y Clapham P Coates G Fairley S Fitzgerald S Ensembl 2011 Nucleic Acids Res 2011 39 Database issue D800 806 21045057 PMC3013672
  70. Fujita PA Rhead B Zweig AS Hinrichs AS Karolchik D Cline MS Goldman M Barber GP Clawson H Coelho A The UCSC Genome Browser database: update 2011 Nucleic Acids Res 2011 39 Database issue D876 882 20959295 PMC3242726
  71. Machanick P Bailey TL MEME-ChIP: motif analysis of large DNA datasets Bioinformatics 2011 27 12 1696 1697 10.1093/bioinformatics/btr189 21486936 PMC3106185 · DOI
  72. Gupta S Stamatoyannopoulos JA Bailey TL Noble WS Quantifying similarity between motifs Genome Biol 2007 8 2 R24 10.1186/gb-2007-8-2-r24 17324271 PMC1852410 · DOI · Paper page
  73. Perona R Montaner S Saniger L Sanchez-Perez I Bravo R Lacal JC Activation of the nuclear factor-kappaB by Rho, CDC42, and Rac-1 proteins Genes Dev 1997 11 4 463 475 10.1101/gad.11.4.463 9042860 · DOI
  74. Al-Shahrour F Minguez P Tarraga J Medina I Alloza E Montaner D Dopazo J FatiGO +: a functional profiling tool for genomic data. Integration of functional annotation, regulatory motifs and interaction data with microarray experiments Nucleic Acids Res 2007 35 Web Server issue W91 96 17478504 PMC1933151
  75. Medina I Carbonell J Pulido L Madeira SC Goetz S Conesa A Tarraga J Pascual-Montano A Nogales-Cadenas R Santoyo J Babelomics: an integrative platform for the analysis of transcriptomics, proteomics and genomic data with advanced functional profiling Nucleic Acids Res 2010 38 Web Server issue W210 213 20478823 PMC2896184

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Authors
Ruiz-Llorente, S., de Pau, E. C. S., Sastre-Perona, A., Montero-Conde, C., Gómez-López, G., Fagin, J. A., Valencia, A., Pisano, D. G., & Santisteban, P.
Affiliations
  • Consejo Superior de Investigaciones Científicas (ES)
  • Memorial Sloan Kettering Cancer Center (US)
  • Spanish National Cancer Research Centre (ES)
Corresponding author
Santisteban, Pilar
ORCID
Venue
BMC Genomics
Publisher
Springer Science and Business Media LLC
Published
2012-04-24
Volume
13
Issue
1
Pages
147-147
ISSN
1471-2164 (ISSN-L)
DOI
10.1186/1471-2164-13-147
PMC
PMC3403905
OpenAlex
W2066819606
Cited by
51
References
2
Citations by year
2026: 1 · 2025: 1 · 2024: 4 · 2023: 5 · 2022: 3 · 2021: 2 · 2020: 5 · 2019: 2 · 2018: 3 · 2016: 8 · 2015: 6 · 2014: 5 · 2013: 5 · 2012: 1
FWCI
2.05
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • BMC Genomics (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
  • Ludwig Institute for Cancer Research
  • Comunidad de Madrid
  • Ministerio de Ciencia e Innovación
  • Instituto de Salud Carlos III
  • National Cancer Institute (R01CA050706, P30CA008748)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Thyroid Disorders and Treatments
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Thyroid Cancer Diagnosis and Treatment
Keywords
PAX8, transcription factor binding sites, CpG islands, CTCF, Sp1, chromatin remodeling, sodium iodide symporter, cell proliferation, apoptosis, cell polarity, transcriptional regulation
MeSH terms
PAX8 Transcription Factor, CCCTC-Binding Factor, Sodium-Iodide Symporters, Animals, Binding Sites, Cell Differentiation, Cell Line, Gene Expression Regulation, HeLa Cells, Humans, Promoter Regions, Genetic, Repressor Proteins, Thyroid Gland, Transfection, Sp1 Transcription Factor, Genome, CpG Islands, Gene Silencing, Symporters, RNA Interference, RNA, Small Interfering, Microarray Analysis, Chromatin Immunoprecipitation, Rats, Paired Box Transcription Factors, Genome-Wide Association Study

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