Cited by 2,623Gene expression and cancer classificationMolecular Biology Techniques and ApplicationsGenomics and Phylogenetic Studies

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The RIN: an RNA integrity number for assigning integrity values to RNA measurements

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Schroeder, Andreas, Mueller, Odilo, Stocker, Susanne, Salowsky, Ruediger, Leiber, Michael, Gassmann, Marcus, Lightfoot, Samar, Menzel, Wolfram, Granzow, Martin, Ragg, Thomas·2006·BMC Molecular Biology

DOI 10.1186/1471-2199-7-3PMC PMC1413964

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Abstract

Background: The integrity of RNA molecules is of paramount importance for experiments that try to reflect the snapshot of gene expression at the moment of RNA extraction. Until recently, there has been no reliable standard for estimating the integrity of RNA samples and the ratio of 28S:18S ribosomal RNA, the common measure for this purpose, has been shown to be inconsistent. The advent of microcapillary electrophoretic RNA separation provides the basis for an automated high-throughput approach, in order to estimate the integrity of RNA samples in an unambiguous way. Methods: A method is introduced that automatically selects features from signal measurements and constructs regression models based on a Bayesian learning technique. Feature spaces of different dimensionality are compared in the Bayesian framework, which allows selecting a final feature combination corresponding to models with high posterior probability. Results: This approach is applied to a large collection of electrophoretic RNA measurements recorded with an Agilent 2100 bioanalyzer to extract an algorithm that describes RNA integrity. The resulting algorithm is a user-independent, automated and reliable procedure for standardization of RNA quality control that allows the calculation of an RNA integrity number (RIN). Conclusion: Our results show the importance of taking characteristics of several regions of the recorded electropherogram into account in order to get a robust and reliable prediction of RNA integrity, especially if compared to traditional methods.

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Mueller O, Lightfoot S, Schröder A: RNA Integrity Number (RIN) Standardization of RNA Quality Control. Tech. Rep. 5989-1165EN, Agilent Technologies, Application Note. 2004, http://www.agilent.com/chem/labonachip
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Authors: Schroeder, A., Mueller, O., Stocker, S., Salowsky, R., Leiber, M., Gassmann, M., Lightfoot, S., Menzel, W., Granzow, M., & Ragg, T.
Affiliations: Agilent Technologies (United States) (US)
Agilent Technologies (Germany) (DE)
Karlsruhe Institute of Technology (DE)
Corresponding author: Mueller, Odilo
Ragg, Thomas
Venue: BMC Molecular Biology
Publisher: Springer Science and Business Media LLC
Published: 2006-01-31
Volume: 7
Issue: 1
Pages: 3-3
ISSN: 1471-2199 (ISSN-L)
DOI: 10.1186/1471-2199-7-3
PMC: PMC1413964
OpenAlex: W1696199194
Cited by: 2,623
References: 18
Citations by year: 2026: 97 · 2025: 106 · 2024: 135 · 2023: 111 · 2022: 132 · 2021: 172 · 2020: 129 · 2019: 108 · 2018: 169 · 2017: 138 · 2016: 166 · 2015: 149 · 2014: 162 · 2013: 180 · 2012: 145
FWCI: 33.21
Type: journal article
Language: en
Open access: hybrid
doi.org
Licence: cc-by
Locations: BMC Molecular Biology (publishedVersion, cc-by)
PubMed Central (publishedVersion, cc-by)
PubMed (publishedVersion)
DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders: Johns Hopkins University
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene expression and cancer classification
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
Keywords: RNA integrity number, RNA quality control, Agilent 2100 Bioanalyzer, Bayesian feature selection, regression models, gene expression
MeSH terms: Algorithms, Animals, Bayes Theorem, Humans, Quality Control, RNA, Software, Linear Models, Polymerase Chain Reaction, Neural Networks, Computer, Gene Expression Profiling, Electrophoresis, Microchip, Mice, Rats

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The RIN: an RNA integrity number for assigning integrity values to RNA measurements

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