The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.
IGF-1 enhances cell proliferation and survival during early differentiation of mesenchymal stem cells to neural progenitor-like cells
Open accessAbstract
BACKGROUND: There has been increasing interest recently in the plasticity of mesenchymal stem cells (MSCs) and their potential to differentiate into neural lineages. To unravel the roles and effects of different growth factors in the differentiation of MSCs into neural lineages, we have differentiated MSCs into neural lineages using different combinations of growth factors. Based on previous studies of the roles of insulin-like growth factor 1 (IGF-1) in neural stem cell isolation in the laboratory, we hypothesized that IGF-1 can enhance proliferation and reduce apoptosis in neural progenitor-like cells (NPCs) during differentiation of MSCs into NCPs.We induced MSCs differentiation under four different combinations of growth factors: (A) EGF + bFGF, (B) EGF + bFGF + IGF-1, (C) EGF + bFGF + LIF, (D) EGF + bFGF + BDNF, and (E) without growth factors, as a negative control. The neurospheres formed were characterized by immunofluorescence staining against nestin, and the expression was measured by flow cytometry. Cell proliferation and apoptosis were also studied by MTS and Annexin V assay, respectively, at three different time intervals (24 hr, 3 days, and 5 days). The neurospheres formed in the four groups were then terminally differentiated into neuron and glial cells. RESULTS: The four derived NPCs showed a significantly higher expression of nestin than was shown by the negative control. Among the groups treated with growth factors, NPCs treated with IGF-1 showed the highest expression of nestin. Furthermore, NPCs derived using IGF-1 exhibited the highest cell proliferation and cell survival among the treated groups. The NPCs derived from IGF-1 treatment also resulted in a better yield after the terminal differentiation into neurons and glial cells than that of the other treated groups. CONCLUSIONS: Our results suggested that IGF-1 has a crucial role in the differentiation of MSCs into neuronal lineage by enhancing the proliferation and reducing the apoptosis in the NPCs. This information will be beneficial in the long run for improving both cell-based and cell-free therapy for neurodegenerative diseases.
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“There are also data that show that BDNF participates in the regulation of neuron metabolism, development, and proper functioning in cooperation with insulin-like growth factor-1 (IGF-1) [201].”
From BDNF as a Promising Therapeutic Agent in Parkinson’s Disease (2020) · cites this paper as [201]
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Cites
Mesenchymal and haematopoietic stem cells form a unique bone marrow niche
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“This is consistent with the previous finding that one third of MSCs died after induction [14].”
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Items 101–129 of 129
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Isolation and Expansion of Adult Canine Hippocampal Neural Precursors
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Effects of IGFBP-2 on proliferation and differentiation in neural stem cell line C17.2
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InfluentialKörpe, D. A., Güler, S., Aydın, H. M., & Duman, M. (2021) · International Journal of Polymeric Materials · doi:10.1080/00914037.2021.2014484 · Cited by 2
“IGF-1 increases cartilaginous matrix production([4]) and cell proliferation([5]).”
BackgroundResearch advances in insulin-like growth factor
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Effect of Skeletal Paracrine Signals on the Proliferation of Interzone Cells
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Proteomic landscape of porcine induced neural stem cell reprogramming and differentiation
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“…in our piNSCs could represent a combination of signals: both from early spontaneously differentiating cells and potentially from an intrinsic upregulation re fl ecting early neural commitment within the progenitor state itself, consistent with these gene expression fi ndings ( Huat et al., 2014 ).”
Fang, Y., Cao, D., Lee, C., Chiu, P. C. N., Ng, E. H. Y., Yeung, W. S. B., & Chan, R. (2025) · Stem Cell Research & Therapy · doi:10.1186/s13287-025-04803-7 · Cited by 1
Neuroscience and Brain Science Special Issue begins in the Malaysian Journal of Medical Sciences.
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Self-citationDisturbances of consciousness in patients subjected to orthopaedic procedure
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Suppression of IGF1R in Melanoma Cells by an Adenovirus-Mediated One-Step Knockdown System
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“Previous study showed enhanced cell proliferation during early differentiation of mesenchymal stem cells to neural progenitor-like cells after IGF1 overexpression.(24) IGF also acts as a key regulator in inhibiting cell apoptosis by controlling Bcl2 family proteins, caspases, and signaling of death-inducing receptors.”
BackgroundLittlejohn, E. L. (2018) · UKnowledge (University of Kentucky) · doi:10.13023/etd.2018.292
Wei, P., Xu, Y., Gu, Y., Yao, Q., Li, J., & Wang, L. (2020) · doi:10.21203/rs.3.rs-28605/v1
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“As known, the increase of IGF-1 expression can promote cell proliferation and differentiation in cells [48, 49].”
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Alketbi, F. S., Khan, A. A., Gul, M. T., Khattak, M. N. K., Jayakumar, M. N., & Samsudin, A. R. (2023) · Advances in Biomedical and Health Sciences · doi:10.4103/abhs.abhs_53_22
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Neural Stem Cell Intervention in Traumatic Brain Injury
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“…factors, were able to differentiate into the three main neural phenotypes: astroglia, oligodendroglia, and neurons (hermann et al. 2004, 2006; huat et al. 2014). lately, the research trend has focused on finding more convenient sources of Mscs as an alternative to conventional ones (e.g.…”
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“Regarding IGF-1, it has been shown that it has a proliferative effect in adult progenitor cells and is necessary for NSPC proliferation (Arsenijevic et al. 2001; Åberg et al. 2003; Huat et al. 2014).”
Receptor Tyrosine Kinase Signaling and Mitochondrial Dynamics in Cancer
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“Insulin-like growth factor-1 (IGF-1) plays a pivotal role in tissue remodeling and development and profoundly affects cell growth, differentiation, proliferation and apop - tosis [11–13 ].”
Khan, A. A., Huat, T. J., Al Mutery, A., El-Serafi, A. T., Kacem, H. H., Abdallah, S. H., Reza, M. F., Abdullah, J. M., & Jaafar, H. (2020) · Figshare · doi:10.6084/m9.figshare.13161651
Self-citationMesenchymal stem cell-related therapies for neurological diseases and disorders
Tebo, E. D., Zehra, M., & Nguyen, H. (2025) · Elsevier eBooks · doi:10.1016/b978-0-443-27414-5.00007-5
Khan, A. A., Huat, T. J., Al Mutery, A., El-Serafi, A. T., Kacem, H. H., Abdallah, S. H., Reza, M. F., Abdullah, J. M., & Jaafar, H. (2020) · Figshare · doi:10.6084/m9.figshare.13161648
Self-citationLiu, X., Huang, L., Guo, Y., Mu, J., Zhu, X., Liu, Z., & Fu, R. (2026) · Frontiers in Oncology · doi:10.3389/fonc.2026.1960550
Metadata
- Authors
- Huat, T. J., Khan, A. A., Pati, S., Mustafa, Z., Abdullah, J. M., & Jaafar, H.
- Affiliations
- Universiti Sains Malaysia (MY)
- Corresponding author
- Huat, Tee Jong
- Venue
- BMC Neuroscience
- Published
- 2014-07-22
- Volume
- 15
- Issue
- 1
- Pages
- 91-91
- ISSN
- 1471-2202 (ISSN-L)
- PMC
- PMC4117972
- OpenAlex
- W2045159954
- Cited by
- 129
- References
- 29
- Citations by year
- 2026: 1 · 2025: 10 · 2024: 9 · 2023: 7 · 2022: 7 · 2021: 13 · 2020: 20 · 2019: 13 · 2018: 9 · 2017: 14 · 2016: 14 · 2015: 9 · 2014: 3
- FWCI
- 6.68
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- BMC Neuroscience (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Greater South Information System
- Funders
- Universiti Sains Malaysia
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Mesenchymal stem cell research
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Keywords
- insulin-like growth factor I, mesenchymal stem cells, neural progenitor-like cells, neuronal differentiation, cell proliferation, apoptosis, growth factor combination, epidermal growth factor, basic fibroblast growth factor, LiF, brain-derived neurotrophic factor, nestin, neurospheres, neurodegenerative disease treatment
- MeSH terms
- Animals, Cell Survival, Cells, Cultured, Insulin-Like Growth Factor I, Neuroglia, Neurons, Rats, Sprague-Dawley, Apoptosis, Intercellular Signaling Peptides and Proteins, Cell Proliferation, Neurogenesis, Neural Stem Cells, Mesenchymal Stem Cells, Nestin
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