Wound healing activities of different extracts of Centella asiatica in incision and burn wound models: an experimental animal study
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Background: The efficacy of Centella asiatica for incision and burn wounds are not fully understood. Here, we report the wound healing activities of sequential hexane, ethyl acetate, methanol, and water extracts of Centella asiatica in incision and partial-thickness burn wound models in rats. Methods: Male Sprague–Dawley rats weighing 250–300 g were randomly divided into incision and burn wound groups. Each group was stratified into seven subgroups: (1) untreated; (2) NSS-; (3) Tween 20®- (vehicle control); (4) hexane extract-; (5) ethyl acetate extract-; (6) methanol extract-; and (7) aqueous extract-treated groups. The test substances were applied topically once daily. The tensile strength of the incision wound was measured on the seventh day after wound infliction. The general appearance and degree of wound healing of the burn wound were assessed on Days 3, 7, 10 and 14 after burn injury and prior to histopathological evaluation. Results: On the seventh day after wound infliction, the tensile strength of incision wound in all extract-treated groups was significantly higher than that of the vehicle control (Tween 20®), but comparable to the NSS-treated group. The degrees of healing in the burn wound with the four extracts were significantly higher than that of the control on Days 3, 10 and 14. Histopathological findings on Day 14 after burn injury revealed prominent fibrinoid necrosis and incomplete epithelialization in the control and untreated groups, whereas fully developed epithelialization and keratinization were observed in all extract-treated groups. Analysis by thin layer chromatography demonstrated that the phyto-constituents β-sitosterol, asiatic acid, and asiaticoside and madecassocide were present in the hexane, ethyl acetate and methanol extracts, respectively. Conclusions: All extracts of Centella asiatica facilitate the wound healing process in both incision and burn wounds. Asiatic acid in the ethyl acetate extract seemed to be the most active component for healing the wound.
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Asiatic acid performed more efficiently compared to asiaticoside and madecassoside in those burn wound models.
“However, asiatic acid was seen to perform more efficiently compared to our phytocompounds of interest.”
From Therapeutic Properties and Pharmacological Activities of Asiaticoside and Madecassoside: A Review (2023) · cites this paper as [102]
The Abstract Conclusions say asiatic acid in the ethyl acetate extract “seemed to be the most active component,” and the Results report earlier burn-wound healing with that extract than with the methanol extract containing asiaticoside and madecassoside. The study compared extracts, not the isolated compounds; its Results also found no significant difference among extract-treated groups on Day 14. Thus it does not establish that asiatic acid itself performed more efficiently across the burn-wound models. (LLM, conf=0.87)
Experimental — accuracy not yet measuredSequential hexane, ethyl acetate, methanol and water extracts of C. asiatica showed remarkable enhancement of burn wound healing with fully developed epithelialization and keratinization in all extract-treated groups in incision and partial-thickness burn wound models in rats.
“All extracts, including those containing asiaticoside, madecassoside and asiatic acid, showed remarkable enhancement of burn wound healing with fully developed epithelialization and keratinization observed in all the extract‐treated groups.”
From Therapeutic Properties and Pharmacological Activities of Asiaticoside and Madecassoside: A Review (2023) · cites this paper as [102]
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- Authors
- Somboonwong, J., Kankaisre, M., Tantisira, B., & Tantisira, M. H.
- Affiliations
- Chulalongkorn University (TH)
- Burapha University (TH)
- Corresponding author
- Tantisira, Mayuree H
- Venue
- BMC Complementary and Alternative Medicine
- Published
- 2012-07-20
- Volume
- 12
- Issue
- 1
- Pages
- 103-103
- ISSN
- 1472-6882 (ISSN-L)
- PMC
- PMC3492213
- OpenAlex
- W2007584755
- Cited by
- 232
- References
- 38
- Citations by year
- 2026: 9 · 2025: 14 · 2024: 24 · 2023: 30 · 2022: 25 · 2021: 30 · 2020: 23 · 2019: 12 · 2018: 14 · 2017: 14 · 2016: 14 · 2015: 11 · 2014: 10 · 2012: 1
- FWCI
- 7.44
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- BMC Complementary and Alternative Medicine (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Greater South Information System
- Funders
- Chulalongkorn University
- National Research Council of Thailand
- Topics
- Health Sciences › Medicine › Rehabilitation › Wound Healing and Treatments
- Health Sciences › Medicine › Complementary and alternative medicine › Medicinal Plants and Neuroprotection
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Medicinal Plant Research
- Keywords
- Centella asiatica, wound healing, incision wound, burn wounds, second-degree burns, plant extracts, asiatic acid, asiaticoside, β-sitosterol, tensile strength, re-epithelialization, keratinization, phytochemical constituents
- MeSH terms
- Animals, Burns, Disease Models, Animal, Humans, Male, Plant Extracts, Postoperative Complications, Skin, Triterpenes, Wound Healing, Rats, Sprague-Dawley, Centella, Rats
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