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In vitro and in vivo wound healing activity of asiaticoside isolated from Centella asiatica
Abstract
The activity of asiaticoside, isolated from Centella asiatica, has been studied in normal as well as delayed-type wound healing. In guinea pig punch wounds topical applications of 0.2% solution of asiaticoside produced 56% increase in hydroxyproline, 57% increase in tensile strength, increased collagen content and better epithelisation. In streptozotocin diabetic rats, where healing is delayed, topical application of 0.4% solution of asiaticoside over punch wounds increased hydroxyproline content, tensile strength, collagen content and epithelisation thereby facilitating the healing. Asiaticoside was active by the oral route also at 1 mg/kg dose in the guinea pig punch wound model. It promoted angiogenesis in the chick chorioallantoic membrane model at 40 microg/disk concentration. These results indicate that asiaticoside exhibits significant wound healing activity in normal as well as delayed healing models and is the main active constituent of Centella asiatica.
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Oral and topical treatment with the alcohol extract of C. asiatica stimulates synthesis, maturation, and crosslinking of collagen in mice.
“Both the oral and topical treatment of the alcohol extract of C. asiatica have been shown to stimulate synthesis, maturation, and the crosslinking of collagen in mice [29].”
From A systematic review of the effect of Centella asiatica on wound healing (2022) · cites this paper as [29]
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Metadata
- Authors
- Shukla, A., Rasik, A. M., Jain, G. K., Shankar, R., Kulshrestha, D. K., & Dhawan, B. N.
- Affiliations
- Central Drug Research Institute (IN)
- Indian Institute of Toxicology Research (IN)
- Corresponding author
- Shukla, A.
- ORCID
- Jain, G.K.
- Venue
- Journal of Ethnopharmacology
- Publisher
- Elsevier BV
- Published
- 1999-04
- Volume
- 65
- Issue
- 1
- Pages
- 1-11
- ISSN
- 0378-8741 (ISSN-L)
- OpenAlex
- W2143088276
- Cited by
- 428
- References
- 16
- Citations by year
- 2026: 9 · 2025: 19 · 2024: 23 · 2023: 16 · 2022: 25 · 2021: 23 · 2020: 22 · 2019: 22 · 2018: 23 · 2017: 15 · 2016: 22 · 2015: 23 · 2014: 13 · 2013: 20 · 2012: 20
- FWCI
- 3.90
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Ethnopharmacology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Council of Scientific and Industrial Research, India
- Topics
- Health Sciences › Medicine › Surgery › Wound Healing and Treatments
- Health Sciences › Medicine › Complementary and alternative medicine › Medicinal Plants and Neuroprotection
- Health Sciences › Medicine › Complementary and alternative medicine › Phytochemicals and Medicinal Plants
- Keywords
- asiaticoside, Centella asiatica, wound healing, delayed wound healing, diabetic wound healing, hydroxyproline, collagen content, tensile strength, re-epithelialization, angiogenesis, chorioallantoic membrane, topical application, oral administration
- MeSH terms
- Administration, Oral, Administration, Topical, Animals, Anti-Infective Agents, Chick Embryo, Guinea Pigs, Male, Models, Biological, Plant Extracts, Streptozocin, Time Factors, Triterpenes, Wound Healing, Wounds and Injuries, Rats, Sprague-Dawley, Rats, In Vitro Techniques
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