Metabolic engineering of Bacillus subtilis for growth on overflow metabolites
Open accessAbstract
Background: The genome of the important industrial host Bacillus subtilis does not encode the glyoxylate shunt, which is necessary to utilize overflow metabolites, like acetate or acetoin, as carbon source. In this study, the operon encoding the isocitrate lyase (aceB) and malate synthase (aceA) from Bacillus licheniformis was transferred into the chromosome of B. subtilis. The resulting strain was examined in respect to growth characteristics and qualities as an expression host. Results: Our results show that the modified B. subtilis strain is able to grow on the C2 compound acetate. A combined transcript, protein and metabolite analysis indicated a functional expression of the native glyoxylate shunt of B. lichenifomis in B. subtilis. This metabolically engineered strain revealed better growth behavior and an improved activity of an acetoin-controlled expression system. Conclusions: The glyoxylate shunt of B. licheniformis can be functionally transferred to B. subtilis. This novel strain offers improved properties for industrial applications, such as growth on additional carbon sources and a greater robustness towards excess glucose feeding.
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Acetate accumulation was detected due to the stimulation of overflow metabolism at these feeding rates.
“acetate accumulation was detected due to the stimulation of overflow metabolism at these feeding rates (Figure S2) [23, 24].”
From The influence of growth rate-controlling feeding strategy on the surfactin production in Bacillus subtilis bioreactor processes (2024) · cites this paper as [24]
The Background links excess glucose and overflow metabolism in B. subtilis to acetate formation, and the Methods say added glucose was used to stimulate acetate formation in a simulated fed-batch experiment. The paper does not report detecting acetate accumulation at the citing study’s feeding rates; its Discussion describes overflow metabolism in the high-glucose experiment as presumptive. No page numbers are available in our copy. (LLM, conf=0.85)
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- Authors
- Kabisch, J., Pratzka, I., Meyer, H., Albrecht, D., Lalk, M., Ehrenreich, A., & Schweder, T.
- Affiliations
- Universität Greifswald (DE)
- Technical University of Munich (DE)
- Corresponding author
- Schweder, Thomas
- Venue
- Microbial Cell Factories
- Published
- 2013-07-25
- Volume
- 12
- Issue
- 1
- Pages
- 72-72
- ISSN
- 1475-2859 (ISSN-L)
- PMC
- PMC3728045
- OpenAlex
- W2130422257
- Cited by
- 55
- References
- 44
- Citations by year
- 2026: 4 · 2025: 5 · 2024: 5 · 2023: 9 · 2022: 2 · 2021: 5 · 2020: 5 · 2019: 2 · 2018: 3 · 2017: 4 · 2016: 4 · 2015: 2 · 2014: 4 · 2013: 1
- FWCI
- 1.64
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- Microbial Cell Factories (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- Bundesministerium für Bildung und Forschung (0315400A/B, 031397A)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Keywords
- Bacillus subtilis, metabolic engineering, glyoxylate cycle, acetate utilization, acetoin, isocitrate lyase, malate synthase, Bacillus licheniformis, glucose tolerance, expression hosts
- MeSH terms
- Bacillus, Bacillus subtilis, Bacterial Proteins, Chromosomes, Bacterial, Glyoxylates, Isocitrate Lyase, Malate Synthase, Operon, RNA, Messenger, Metabolic Engineering
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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