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Spatial and temporal variation in the kdr allele L1014S in Anopheles gambiae s.s. and phenotypic variability in susceptibility to insecticides in Western Kenya
Open accessAbstract
Background: Malaria vector control in Africa depends upon effective insecticides in bed nets and indoor residual sprays. This study investigated the extent of insecticide resistance in Anopheles gambiae s.l., Anopheles gambiae s.s. and Anopheles arabiensis in western Kenya where ownership of insecticide-treated bed nets has risen steadily from the late 1990s to 2010. Temporal and spatial variation in the frequency of a knock down resistance (kdr) allele in A. gambiae s.s. was quantified, as was variation in phenotypic resistance among geographic populations of A. gambiae s.l. Methods: To investigate temporal variation in kdr frequency, individual specimens of A. gambiae s.s. from two sentinel sites were genotyped using RT-PCR from 1996-2010. Spatial variation in kdr frequency, species composition, and resistance status were investigated in additional populations of A. gambiae s.l. sampled in western Kenya in 2009 and 2010. Specimens were genotyped for kdr as above and identified to species via conventional PCR. Field-collected larvae were reared to adulthood and tested for insecticide resistance using WHO bioassays. Results: Anopheles gambiae s.s. showed a dramatic increase in kdr frequency from 1996 - 2010, coincident with the scale up of insecticide-treated nets. By 2009-2010, the kdr L1014S allele was nearly fixed in the A. gambiae s.s. population, but was absent in A. arabiensis. Near Lake Victoria, A. arabiensis was dominant in samples, while at sites north of the lake A. gambiae s.s was more common but declined relative to A. arabiensis from 2009 to 2010. Bioassays demonstrated that A. gambiae s.s. had moderate phenotypic levels of resistance to DDT, permethrin and deltamethrin while A. arabiensis was susceptible to all insecticides tested. Conclusions: The kdr L1014S allele has approached fixation in A. gambiae s.s. populations of western Kenya, and these same populations exhibit varying degrees of phenotypic resistance to DDT and pyrethroid insecticides. The near absence of A. gambiae s.s. from populations along the lakeshore and the apparent decline in other populations suggest that insecticide-treated nets remain effective against this mosquito despite the increase in kdr allele frequency. The persistence of A. arabiensis, despite little or no detectable insecticide resistance, is likely due to behavioural traits such as outdoor feeding and/or feeding on non-human hosts by which this species avoids interaction with insecticide-treated nets.
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“According to Mathias et al. [32], ITN coverage for populations has been associated with a development of strong insecticide selection pressure by pyrethroid and increase of kdr allele frequency.”
From Mapping insecticide resistance in Anopheles gambiae (s.l.) from Côte d'Ivoire (2018) · cites this paper as [32]
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“Currently, the increasing use of insecticides for malaria vector control plays an important role in the insecticide selection pressure.”
From Mapping insecticide resistance in Anopheles gambiae (s.l.) from Côte d'Ivoire (2018) · cites this paper as [32]
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Metadata
- Authors
- Mathias, D. K., Ochomo, E., Atieli, F., Ombok, M., Nabie Bayoh, M., Olang, G., Muhia, D., Kamau, L., Vulule, J. M., Hamel, M. J., Hawley, W. A., Walker, E. D., & Gimnig, J. E.
- Affiliations
- Kenya Medical Research Institute (KE)
- Centers for Disease Control and Prevention (US)
- Michigan State University (US)
- Corresponding author
- Walker, Edward D
- Gimnig, John E
- Venue
- Malaria Journal
- Published
- 2011-01-14
- Volume
- 10
- Issue
- 1
- Pages
- 10-10
- ISSN
- 1475-2875 (ISSN-L)
- PMC
- PMC3029224
- OpenAlex
- W2106675599
- Cited by
- 158
- References
- 56
- Citations by year
- 2026: 5 · 2025: 4 · 2024: 7 · 2023: 5 · 2022: 3 · 2021: 20 · 2020: 13 · 2019: 9 · 2018: 14 · 2017: 8 · 2016: 12 · 2015: 11 · 2014: 13 · 2013: 15 · 2012: 14
- FWCI
- 14.78
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- Malaria Journal (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Kenyatta University Institutional Repository (Kenyatta University) (submittedVersion)
- Funders
- National Science Foundation (EF-072377)
- Bill and Melinda Gates Foundation
- Centers for Disease Control and Prevention
- Topics
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Malaria Research and Control
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Insect Resistance and Genetics
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Dengue and Mosquito Control Research
- Keywords
- Anopheles arabiensis, knockdown resistance, insecticide resistance, pyrethroid resistance, DDT resistance, permethrin, deltamethrin, insecticide-treated nets, malaria vector control, species composition, spatiotemporal variation, Western Kenya
- MeSH terms
- Animals, Anopheles, Cattle, Child, Preschool, Drug Resistance, Female, Gene Frequency, Geography, Humans, Infant, Infant, Newborn, Insecticides, Kenya, Pregnancy, Time Factors, Insect Proteins, Amino Acid Substitution, Reverse Transcriptase Polymerase Chain Reaction
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