Performance of a HRP-2/pLDH based rapid diagnostic test at the Bangladesh-India-Myanmar border areas for diagnosis of clinical malaria

Elahi, Rubayet; Mohon, Abu Naser; Khan, Wasif A; Haque, Rashidul; Alam, Mohammad Shafiul

doi:10.1186/1475-2875-12-378

Cited by 10Malaria Research and ControlBiosensors and Analytical DetectionClinical Laboratory Practices and Quality Control

Performance of a HRP-2/pLDH based rapid diagnostic test at the Bangladesh-India-Myanmar border areas for diagnosis of clinical malaria

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Elahi, Rubayet, Mohon, Abu Naser, Khan, Wasif A, Haque, Rashidul, Alam, Mohammad Shafiul·2013·Malaria Journal

DOI 10.1186/1475-2875-12-378PMC PMC4228469

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Abstract

Background: The rapid diagnostic test (RDT) has been adopted in contemporary malaria control and management programmes around the world as it represents a fast and apt alternative for malaria diagnosis in a resource-limited setting. This study assessed the performance of a HRP-2/pLDH based RDT (Parascreen® Pan/Pf) in a laboratory setting utilizing clinical samples obtained from the field. Methods: Whole blood samples were obtained from febrile patients referred for malaria diagnosis by clinicians from two different Upazila Health Complexes (UHCs) located near the Bangladesh-India and Bangladesh-Myanmar border where malaria is endemic. RDT was performed on archived samples and sensitivity and specificity evaluated with expert microscopy (EM) and quantitative PCR (qPCR). Results: A total of 327 clinical samples were made available for the study, of which 153 were Plasmodium falciparum-positive and 54 were Plasmodium vivax-positive. In comparison with EM, for P. falciparum malaria, the RDT had sensitivity: 96.0% (95% CI, 91.2-98.3) and specificity: 98.2% (95% CI, 94.6-99.5) and for P. vivax, sensitivity: 90.7% (95% CI, 78.9-96.5) and specificity: 98.9% (95% CI, 96.5-99.7). Comparison with qPCR showed, for P. falciparum malaria, sensitivity: 95.4% (95% CI, 90.5-98.0) and specificity: 98.8% (95% CI, 95.4-99.7) and for P. vivax malaria, sensitivity: 89.0% (95% CI,77.0-95.4) and specificity: 98.8% (95% CI, 96.5-99.7). Sensitivity varied according to different parasitaemia for falciparum and vivax malaria diagnosis. Conclusion: Parascreen® Pan/Pf Rapid test for malaria showed acceptable sensitivity and specificity in border belt endemic areas of Bangladesh when compared with EM and qPCR.

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The few studies to evaluate RDT for P. vivax infection in non-pregnant individuals report varying performance.
“The few studies to evaluate RDT for P. vivax infection in non-pregnant individuals report varying performance [19, 20, 44].”
From Accuracy of an HRP-2/panLDH rapid diagnostic test to detect peripheral and placental Plasmodium falciparum infection in Papua New Guinean women with anaemia or suspected malaria (2015) · cites this paper as [19]

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Authors: Elahi, R., Mohon, A. N., Khan, W. A., Haque, R., & Alam, M. S.
Affiliations: International Centre for Diarrhoeal Disease Research (BD)
Corresponding author: Alam, Mohammad Shafiul
ORCID: Elahi, Rubayet
Khan, Wasif A
Haque, Rashidul
Alam, Mohammad Shafiul
Venue: Malaria Journal
Publisher: Springer Science and Business Media LLC
Published: 2013-10-30
Volume: 12
Issue: 1
Pages: 378-378
ISSN: 1475-2875 (ISSN-L)
DOI: 10.1186/1475-2875-12-378
PMC: PMC4228469
OpenAlex: W2138418569
Cited by: 10
References: 33
Citations by year: 2024: 1 · 2020: 1 · 2018: 2 · 2016: 1 · 2015: 3 · 2014: 1 · 2013: 1
FWCI: 2.04
Type: journal article
Language: en
Open access: gold
doi.org
Licence: cc-by
Locations: Malaria Journal (publishedVersion, cc-by)
Europe PMC (PubMed Central) (publishedVersion, cc-by)
PubMed Central (publishedVersion, cc-by)
PubMed (publishedVersion)
Greater South Information System
Funders: United States Agency for International Development
Styrelsen för Internationellt Utvecklingssamarbete
Department for International Development, UK Government
Australian Agency for International Development
Department for International Development
International Centre for Diarrhoeal Disease Research, Bangladesh
Topics: Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Malaria Research and Control
Physical Sciences › Engineering › Biomedical Engineering › Biosensors and Analytical Detection
Health Sciences › Medicine › Physiology › Clinical Laboratory Practices and Quality Control
Keywords: malaria rapid diagnostic tests, Plasmodium falciparum, Plasmodium vivax, sensitivity and specificity, febrile malaria, India-Bangladesh border, parasitemia
MeSH terms: Antibodies, Protozoan, Antigens, Protozoan, Bangladesh, Myanmar, Diagnostic Tests, Routine, Humans, Immunoassay, India, L-Lactate Dehydrogenase, Sensitivity and Specificity, Protozoan Proteins, Malaria, Falciparum, Malaria, Vivax, Point-of-Care Systems

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Performance of a HRP-2/pLDH based rapid diagnostic test at the Bangladesh-India-Myanmar border areas for diagnosis of clinical malaria

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