CoChat
Sign Up
Cited by 157Essential Oils and Antimicrobial ActivityAntimicrobial Resistance in StaphylococcusAntibiotic Resistance in Bacteria

The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.

Antibacterial activity against β- lactamase producing Methicillin and Ampicillin-resistants Staphylococcus aureus: fractional Inhibitory Concentration Index (FICI) determination

Open access
Konaté, Kiessoun, Mavoungou, Jacques, Lepengué, Alexis, Aworet-Samseny, Raïssa RR, Hilou, Adama, Souza, Alain, Dicko, Mamoudou H, M’Batchi, Bertrand·2012·Annals of Clinical Microbiology and Antimicrobials
DOI 10.1186/1476-0711-11-18PMC PMC3464800
Open this paper in CoChat — free accountOpen PDF

Abstract

BACKGROUND: The present study reports the antibacterial capacity of alkaloid compounds in combination with Methicillin and Ampicillin-resistants bacteria isolated from clinical samples. The resistance of different bacteria strains to the current antibacterial agents, their toxicity and the cost of the treatment have led to the development of natural products against the bacteria resistant infections when applied in combination with conventional antimicrobial drugs. METHOD: The antibacterial assays in this study were performed by using inhibition zone diameters, MIC, MBC methods, the time-kill assay and the Fractional Inhibitory Concentration Index (FICI) determination. On the whole, fifteen Gram-positive bacterial strains (MRSA/ARSA) were used. Negative control was prepared using discs impregnated with 10 % DMSO in water and commercially available Methicillin and Ampicillin from Alkom Laboratories LTD were used as positive reference standards for all bacterial strains. RESULTS: We noticed that the highest activities were founded with the combination of alkaloid compounds and conventional antibiotics against all bacteria strains. Then, results showed that after 7 h exposition there was no viable microorganism in the initial inoculums. CONCLUSION: The results of this study showed that alkaloid compounds in combination with conventional antibiotics (Methicillin, Ampicillin) exhibited antimicrobial effects against microorganisms tested. These results validate the ethno-botanical use of Cienfuegosia digitata Cav. (Malvaceae) in Burkina Faso. Moreover, this study demonstrates the potential of this herbaceous as a source of antibacterial agent that could be effectively used for future health care purposes.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1186/1476-0711-11-18

Cites

29 of 29 references verified0 citation errors · 0 not checkable

Statistics

Figures

Figure 1 Inhibition Zone Diameters of Alkaloid compounds fromCienfuegosia digitataCav. and conventional antibiotics (Methicillin and Ampicillin). The results are the means of number of the colonies ± standard deviations.
Figure 1 Inhibition Zone Diameters of Alkaloid compounds fromCienfuegosia digitataCav. and conventional antibiotics (Methicillin and Ampicillin). The results are the means of number of the colonies ± standard deviations.CC BY · PubMed Central
Figure 2 Inhibition Zone Diameters of Alkaloid compounds and their combination with conventional antibiotics (Methicillin and Ampicillin). Amp= Ampicillin; Meth= Methicillin; Alk= Alkaloids. The results are the means of number of the colonies ± standard deviations.
Figure 2 Inhibition Zone Diameters of Alkaloid compounds and their combination with conventional antibiotics (Methicillin and Ampicillin). Amp= Ampicillin; Meth= Methicillin; Alk= Alkaloids. The results are the means of number of the colonies ± standard deviations.CC BY · PubMed Central
Figure 3 Minimum Inhibitory Concentration (MIC) of alkaloid compounds fromCienfuegosia digitataCav. and their combination with Methicillin and with Ampicillin. The results are the means of number of the colonies ± standard deviations.
Figure 3 Minimum Inhibitory Concentration (MIC) of alkaloid compounds fromCienfuegosia digitataCav. and their combination with Methicillin and with Ampicillin. The results are the means of number of the colonies ± standard deviations.CC BY · PubMed Central
Figure 4 Minimum bactericidal Concentration (MBC) of alkaloid compounds fromCienfuegosia digitataCav. and their combination with Methicillin and with Ampicillin. The results are the means of number of the colonies ± standard deviations.
Figure 4 Minimum bactericidal Concentration (MBC) of alkaloid compounds fromCienfuegosia digitataCav. and their combination with Methicillin and with Ampicillin. The results are the means of number of the colonies ± standard deviations.CC BY · PubMed Central
Figure 5 Viability of microorganisms after 6 hours exposure with Alkaloid compounds fromCienfuegosia digitataCav. Absence of colonies after 6 h. CFU uncountable before 2 h. The results are the means of number of the colonies ± standard deviations.
Figure 5 Viability of microorganisms after 6 hours exposure with Alkaloid compounds fromCienfuegosia digitataCav. Absence of colonies after 6 h. CFU uncountable before 2 h. The results are the means of number of the colonies ± standard deviations.CC BY · PubMed Central
Figure 6 Viability of microorganisms after 6 hours exposure with Methicillin in combination and Alkaloid compounds fromCienfuegosia digitataCav. Absence of colonies after 6 h. UFC uncountable before 2 h. The results are the means of number of the colonies ± standard deviations.
Figure 6 Viability of microorganisms after 6 hours exposure with Methicillin in combination and Alkaloid compounds fromCienfuegosia digitataCav. Absence of colonies after 6 h. UFC uncountable before 2 h. The results are the means of number of the colonies ± standard deviations.CC BY · PubMed Central
Figure 7 Viability of microorganisms after 6 hours exposure with Ampicillin in combination and Alkaloid compounds fromCienfuegosia digitataCav. Absence of colonies after 6 h. UFC uncountable before 2 h. The results are the means of number of the colonies ± standard deviations.
Figure 7 Viability of microorganisms after 6 hours exposure with Ampicillin in combination and Alkaloid compounds fromCienfuegosia digitataCav. Absence of colonies after 6 h. UFC uncountable before 2 h. The results are the means of number of the colonies ± standard deviations.CC BY · PubMed Central

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Show all 29 references

Research works printed but not linked: 2

  1. 10.1016/S0378-8741(97)00123-2 · DOI · Paper page
  2. 10.1007/s00203-002-0436-0 · DOI

Cited by

Sorted by citations

Items 101–157 of 157

Related

See all

Metadata

Authors
Konaté, K., Mavoungou, J., Lepengué, A., Aworet-Samseny, R. R., Hilou, A., Souza, A., Dicko, M. H., & M’Batchi, B.
Affiliations
  • Université Joseph Ki-Zerbo (BF)
  • Université des Sciences et Techniques de Masuku (GA)
Corresponding author
Konaté, Kiessoun
ORCID
Venue
Annals of Clinical Microbiology and Antimicrobials
Publisher
Springer Science and Business Media LLC
Published
2012
Volume
11
Issue
1
Pages
18
ISSN
1476-0711 (ISSN-L)
DOI
10.1186/1476-0711-11-18
PMC
PMC3464800
OpenAlex
W2142832600
Cited by
155
References
29
Citations by year
2026: 11 · 2025: 18 · 2024: 16 · 2023: 21 · 2022: 17 · 2021: 20 · 2020: 12 · 2019: 10 · 2018: 10 · 2017: 4 · 2016: 5 · 2015: 2 · 2014: 5 · 2013: 4
FWCI
5.41
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Annals of Clinical Microbiology and Antimicrobials (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
  • Health Sciences › Medicine › Infectious Diseases › Antimicrobial Resistance in Staphylococcus
  • Health Sciences › Medicine › Microbiology (medical) › Antibiotic Resistance in Bacteria
Keywords
Staphylococcus aureus, methicillin resistance, ampicillin resistance, beta-lactamase, fractional inhibitory concentration index, combination antibiotic therapy, Malvaceae, antibacterial activity, time-kill assay, ethnobotany, Burkina Faso
MeSH terms
Alkaloids, Ampicillin, Ampicillin Resistance, Anti-Bacterial Agents, Bacterial Proteins, beta-Lactamases, Biological Assay, Drug Synergism, Methicillin, Microbial Sensitivity Tests, Plant Extracts, Methicillin Resistance, Malvaceae, Methicillin-Resistant Staphylococcus aureus, beta-Lactamase Inhibitors

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review