Ovarian steroids regulate tachykinin and tachykinin receptor gene expression in the mouse uterus
Open accessAbstract
Background: In the mouse uterus, pregnancy is accompanied by changes in tachykinin and tachykinin receptor gene expression and in the uterotonic effects of endogenous tachykinins. In this study we have investigated whether changes in tachykinin expression and responses are a result of changes in ovarian steroid levels. Methods: We quantified the mRNAs of tachykinins and tachykinin receptors in uteri from ovariectomized mice and studied their regulation in response to estrogen and progesterone using real-time quantitative RT-PCR. Early (3 h) and late (24 h) responses to estrogen were evaluated and the participation of the estrogen receptors (ER), ERalpha and ERbeta, was analyzed by treating mice with propylpyrazole triol, a selective ERalpha agonist, or diarylpropionitrile, a selective agonist of ERbeta. Results: All genes encoding tachykinins (Tac1, Tac2 and Tac4) and tachykinin receptors (Tacr1, Tacr2 and Tacr3) were expressed in uteri from ovariectomized mice. Estrogen increased Tac1 and Tacr1 mRNA after 3 h and decreased Tac1 and Tac4 expression after 24 h. Tac2 and Tacr3 mRNA levels were decreased by estrogen at both 3 and 24 h. Most effects of estrogen were also observed in animals treated with propylpyrazole triol. Progesterone treatment increased the levels of Tac2. Conclusion: These results show that the expression of tachykinins and their receptors in the mouse uterus is tightly and differentially regulated by ovarian steroids. Estrogen effects are mainly mediated by ERalpha supporting an essential role for this estrogen receptor in the regulation of the tachykinergic system in the mouse uterus.
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Expression of NK1R and SP-coding gene TAC1 is upregulated by estrogen and TNF-α.
“The expression of NK1R and SP-coding gene TAC1 is reported to be upregulated by estrogen68,71 and TNF-α44.”
From Neuropeptides Substance P and Calcitonin Gene Related Peptide Accelerate the Development and Fibrogenesis of Endometriosis (2019) · cites this paper as [71]
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- Authors
- Pinto, F. M., Pintado, C. O., Pennefather, J. N., Patak, E., & Candenas, L.
- Affiliations
- Consejo Superior de Investigaciones Científicas (ES)
- Universidad de Sevilla (ES)
- Monash University (AU)
- Royal Women's Hospital (AU)
- Corresponding author
- Candenas, Luz
- Venue
- Reproductive Biology and Endocrinology
- Published
- 2009-07-23
- Volume
- 7
- Issue
- 1
- Pages
- 77-77
- ISSN
- 1477-7827 (ISSN-L)
- PMC
- PMC2724541
- OpenAlex
- W2136380625
- Cited by
- 29
- References
- 46
- Citations by year
- 2026: 1 · 2025: 1 · 2023: 2 · 2022: 3 · 2020: 2 · 2019: 4 · 2018: 1 · 2017: 1 · 2016: 1 · 2015: 1 · 2014: 3 · 2013: 3 · 2012: 2
- FWCI
- 0.83
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- Reproductive Biology and Endocrinology (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- Junta de Andalucía
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
- Keywords
- tachykinins, tachykinin receptors, ovarian steroids, estrogen, progesterone, uterus, estrogen receptor alpha, estrogen receptor beta, TAC1, neurokinin-1 receptor, TACR3, ovariectomy, pregnancy, gene expression regulation, propyl pyrazole triol, diarylpropionitrile
- MeSH terms
- Animals, Estradiol, Female, Nitriles, Ovary, Phenols, Progesterone, Propionates, Pyrazoles, Uterus, Tachykinins, Gene Expression, Receptors, Tachykinin, Estrogen Receptor alpha, Mice
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