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Cited by 100Sperm and Testicular FunctionMale Reproductive Health StudiesReproductive biology and impacts on aquatic species

Sperm DNA fragmentation and oxidation are independent of malondialdheyde

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Zribi, Nassira, Chakroun, Nozha, Elleuch, Henda, Abdallah, Fatma, Ben Hamida, Afifa, Gargouri, Jalel, Fakhfakh, Faiza, Keskes, Leila·2011·Reproductive Biology and Endocrinology
DOI 10.1186/1477-7827-9-47PMC PMC3098153
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Abstract

BACKGROUND: There is clinical evidence to show that sperm DNA damage could be a marker of sperm quality and extensive data exist on the relationship between DNA damage and male fertility status. Detecting such damage in sperm could provide new elements besides semen parameters in diagnosing male infertility. We aimed to assess sperm DNA fragmentation and oxidation and to study the association between these two markers, routine semen parameters and malondialdehyde formation. METHODS: Semen samples from 55 men attending the Histology-Embryology Laboratory of Sfax Faculty of Medicine, Tunisia, for semen investigations were analysed for sperm DNA fragmentation and oxidation using flow cytometry. The Sperm was also assessed spectrophotometrically for malondialdehyde formation. RESULTS: Within the studied group, 21 patients were nonasthenozoospermic (sperm motility ≥ 50%) and 34 patients were considered asthenozoospermic (sperm motility < 50%). A positive correlation was found between sperm DNA fragmentation and oxidation (p = 0.01; r = 0.33). We also found a negative correlation between sperm DNA fragmentation and some sperm parameters: total motility (p = 0.001; r = -0.43), rapid progressive motility (type a motility) (p = 0.04; r = -0.27), slow progressive motility (type b motility) (p = 0.03; r = -0.28), and vitality (p < 0.001; r = -0.65). Sperm DNA fragmentation was positively correlated with coiled tail (p = 0.01; r = 0.34). The two parameters that were found to be correlated with oxidative DNA damage were leucocytes concentrations (p = 0.01; r = 0.38) and broken neck (p = 0.02; r = 0.29). Sperm MDA levels were negatively correlated with sperm concentration (p < 0.001; r = -0.57), total motility (p = 0.01; r = -0.35) and type a motility (p = 0.03; r = -0.32); but not correlated with DNA fragmentation and DNA oxidation. CONCLUSIONS: Our results support the evidence that oxidative stress plays a key role in inducing DNA damage; but nuclear alterations and malondialdehyde don't seem to be synchronous.

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  • DNA fragmentation was negatively correlated with several parameters of sperm motility and vitality.

    “DNA fragmentation was found to be negatively correlated with several parameters of sperm motility and vitality.”

    From Iron, Oxidative Stress and Health (2012) · cites this paper as Zribi et al., (2011)

  • In a study comparing 21 nonasthenozoospermic men (sperm motility ≥50%) with 34 asthenozoospermic men (sperm motility <50%), oxidation was positively correlated with DNA fragmentation.

    “Zribi et al., (2011) recently reported a positive correlation between oxidation and DNA fragmentation in a study wherein 21 nonasthenozoospermic (sperm motility ≥ 50%) were compared with 34 asthenozoospermic (sperm motility < 50%).”

    From Iron, Oxidative Stress and Health (2012) · cites this paper as Zribi et al., (2011)

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Cited by

Sorted by citations

Authors
Zribi, N., Chakroun, N., Elleuch, H., Abdallah, F., Ben Hamida, A., Gargouri, J., Fakhfakh, F., & Keskes, L.
Affiliations
University of Sfax (TN)
Corresponding author
Zribi, Nassira
ORCID
Venue
Reproductive Biology and Endocrinology
Publisher
Springer Science and Business Media LLC
Published
2011
Volume
9
Issue
1
Pages
47
ISSN
1477-7827 (ISSN-L)
DOI
10.1186/1477-7827-9-47
PMC
PMC3098153
OpenAlex
W2156891756
Cited by
100
References
0
Citations by year
2026: 4 · 2025: 5 · 2024: 3 · 2023: 8 · 2022: 13 · 2021: 8 · 2020: 7 · 2019: 3 · 2018: 7 · 2017: 5 · 2016: 7 · 2015: 8 · 2014: 5 · 2013: 6 · 2012: 10
FWCI
7.89
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Reproductive Biology and Endocrinology (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Topics
  • Health Sciences › Medicine › Reproductive Medicine › Sperm and Testicular Function
  • Health Sciences › Health Professions › General Health Professions › Male Reproductive Health Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Reproductive biology and impacts on aquatic species
Keywords
sperm DNA fragmentation, oxidative DNA damage, malondialdehyde, oxidative stress, male infertility, sperm motility, sperm viability, sperm morphology, semen parameters, asthenozoospermia, leukocyte contamination, sperm quality markers
MeSH terms
Adult, DNA Damage, Humans, Infertility, Male, Male, Malondialdehyde, Middle Aged, Oxidation-Reduction, Sperm Motility, Spermatozoa, Lipid Peroxidation, In Situ Nick-End Labeling, Asthenozoospermia, DNA Fragmentation, Semen Analysis

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