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Proteomic analysis of human vitreous humor
Open accessAbstract
BACKGROUND: The vitreous humor is a transparent, gelatinous mass whose main constituent is water. It plays an important role in providing metabolic nutrient requirements of the lens, coordinating eye growth and providing support to the retina. It is in close proximity to the retina and reflects many of the changes occurring in this tissue. The biochemical changes occurring in the vitreous could provide a better understanding about the pathophysiological processes that occur in vitreoretinopathy. In this study, we investigated the proteome of normal human vitreous humor using high resolution Fourier transform mass spectrometry. RESULTS: The vitreous humor was subjected to multiple fractionation techniques followed by LC-MS/MS analysis. We identified 1,205 proteins, 682 of which have not been described previously in the vitreous humor. Most proteins were localized to the extracellular space (24%), cytoplasm (20%) or plasma membrane (14%). Classification based on molecular function showed that 27% had catalytic activity, 10% structural activity, 10% binding activity, 4% cell and 4% transporter activity. Categorization for biological processes showed 28% participate in metabolism, 20% in cell communication and 13% in cell growth. The data have been deposited to the ProteomeXchange with identifier PXD000957. CONCLUSION: This large catalog of vitreous proteins should facilitate biomedical research into pathological conditions of the eye including diabetic retinopathy, retinal detachment and cataract.
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“proteomic studies of the eye reveal that ocular tissues, including the retina, shed intracellular proteins into adjacent intraocular fluid compartments (e.g., the vitreous fluid; Fig. 1a)20,26–28.”
From Investigation of Cas9 Antibodies in the Human Eye (2022) · cites this paper as [28]
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“This tissue makes up 80% of the human eye by volume and sequesters both intracellular and extracellular proteins shed from the retina and adjacent tissues20,26–28.”
From Investigation of Cas9 Antibodies in the Human Eye (2022) · cites this paper as [28]
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“This tissue makes up 80% of the human eye by volume and sequesters both intracellular and extracellular proteins shed from the retina and adjacent tissues20,26–28.”
From Investigation of Cas9 Antibodies in the Human Eye (2022) · cites this paper as [28]
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Metadata
- Authors
- Murthy, K. R., Goel, R., Subbannayya, Y., Jacob, H. K., Murthy, P. R., Manda, S. S., Patil, A. H., Sharma, R., Sahasrabuddhe, N. A., Parashar, A., Nair, B. G., Krishna, V., Prasad, T. K., Gowda, H., & Pandey, A.
- Affiliations
- Institute of Bioinformatics (IN)
- Kuvempu University (IN)
- Pondicherry University (IN)
- National Institute of Mental Health and Neurosciences (IN)
- Armed Forces Medical College (IN)
- Amrita Vishwa Vidyapeetham (IN)
- Johns Hopkins University (US)
- Corresponding author
- Murthy, Krishna R
- ORCID
- Venue
- Clinical Proteomics
- Published
- 2014-07-14
- Volume
- 11
- Issue
- 1
- Pages
- 29-29
- ISSN
- 1542-6416 (ISSN-L)
- 1559-0275
- PMC
- PMC4106660
- OpenAlex
- W2164518567
- Cited by
- 145
- References
- 76
- Citations by year
- 2026: 6 · 2025: 10 · 2024: 14 · 2023: 12 · 2022: 9 · 2021: 10 · 2020: 21 · 2019: 8 · 2018: 18 · 2017: 15 · 2016: 14 · 2015: 6 · 2014: 2
- FWCI
- 5.29
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- Clinical Proteomics (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- Wellcome Trust
- Department of Biotechnology, Ministry of Science and Technology, India
- University Grants Commission
- The Wellcome Trust DBT India Alliance
- Topics
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Health Sciences › Medicine › Ophthalmology › Retinal Diseases and Treatments
- Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
- Keywords
- vitreous humor, vitreous proteome, vitreoretinopathy, vitreous proteins, extracellular proteins, protein function classification, diabetic retinopathy, retinal detachment, cataract
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