Phosphatidic acid enhances mTOR signaling and resistance exercise induced hypertrophy
Open accessAbstract
INTRODUCTION: The lipid messenger phosphatidic acid (PA) plays a critical role in the stimulation of mTOR signaling. However, the mechanism by which PA stimulates mTOR is currently unknown. Therefore, the purpose of this study was to compare the effects of various PA precursors and phospholipids on their ability to stimulate mTOR signaling and its ability to augment resistance training-induced changes in body composition and performance. METHODS: In phase one, C2C12 myoblasts cells were stimulated with different phospholipids and phospholipid precursors derived from soy and egg sources. The ratio of phosphorylated p70 (P-p70-389) to total p70 was then used as readout for mTOR signaling. In phase two, resistance trained subjects (n = 28, 21 ± 3 years, 77 ± 4 kg, 176 ± 9 cm) consumed either 750 mg PA daily or placebo and each took part in an 8 week periodized resistance training program. RESULTS: In phase one, soy-phosphatidylserine, soy-Lyso-PA, egg-PA, and soy-PA stimulated mTOR signaling, and the effects of soy-PA (+636%) were significantly greater than egg-PA (+221%). In phase two, PA significantly increased lean body mass (+2.4 kg), cross sectional area (+1.0 cm), and leg press strength (+51.9 kg) over placebo. CONCLUSION: PA significantly activates mTOR and significantly improved responses in skeletal muscle hypertrophy, lean body mass, and maximal strength to resistance exercise.
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PA supplementation (750mg/day) during 8 weeks resistance training lead to significant increases in leg press strength (+51.9 kg) compared to placebo.
“and leg press strength (+51.9 kg) when compared to placebo [184].”
From New strategies in sport nutrition to increase exercise performance (2016) · cites this paper as [184]
PA supplementation (750mg/day) during 8 weeks resistance training lead to significant increases in lean body mass (+2.4 kg) compared to placebo.
“PA supplementation (750mg/day) during 8 weeks resistance training lead to significant increases in lean body mass (+2.4 kg)”
From New strategies in sport nutrition to increase exercise performance (2016) · cites this paper as [184]
PA supplementation (750mg/day) during 8 weeks resistance training lead to significant increases in skeletal muscle cross sectional area (+1.0 cm) compared to placebo.
“skeletal muscle cross sectional area (+1.0 cm)”
From New strategies in sport nutrition to increase exercise performance (2016) · cites this paper as [184]
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Nutraceuticals Present in Edible Oils
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The effects of phosphatidic acid on performance and body composition - a scoping review
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- Authors
- Joy, J. M., Gundermann, D. M., Lowery, R. P., Jäger, R., McCleary, S. A., Purpura, M., Roberts, M. D., Wilson, S. M., Hornberger, T. A., & Wilson, J. M.
- Affiliations
- University of Tampa (US)
- University of Wisconsin–Madison (US)
- Increnovo LLC (United States) (US)
- Auburn University (US)
- Corresponding author
- Wilson, Jacob M
- ORCID
- Venue
- Nutrition & Metabolism
- Published
- 2014-06-16
- Volume
- 11
- Issue
- 1
- Pages
- 29-29
- ISSN
- 1743-7075 (ISSN-L)
- PMC
- PMC4066292
- OpenAlex
- W2099081622
- Cited by
- 76
- References
- 36
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 3 · 2023: 5 · 2022: 2 · 2021: 5 · 2020: 9 · 2019: 4 · 2018: 12 · 2017: 8 · 2016: 10 · 2015: 11
- FWCI
- 4.82
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- Nutrition & Metabolism (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- University of Wisconsin-Madison
- University of Tampa
- National Institutes of Health (AR057347)
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (AR-057347, R01AR057347)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › PI3K/AKT/mTOR signaling in cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Keywords
- phosphatidic acid, mTOR signaling, resistance training, skeletal muscle hypertrophy, phospholipids, phosphatidylserine, lysophosphatidic acid, soy phospholipids, egg phospholipids, p70S6K phosphorylation, lean body mass, leg press strength, dietary supplementation
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