The DOI matches its Crossref record; 54 of the 54 references it cites were found in a registry or an index.
Lipid accumulation and biosynthesis genes response of the oleaginous Chlorella pyrenoidosaunder three nutrition stressors
Open accessAbstract
Background: Microalgae can accumulate considerable amounts of lipids under different nutrient-deficient conditions, making them as one of the most promising sustainable sources for biofuel production. These inducible processes provide a powerful experimental basis for fully understanding the mechanisms of physiological acclimation, lipid hyperaccumulation and gene expression in algae. In this study, three nutrient-deficiency strategies, viz nitrogen-, phosphorus- and iron-deficiency were applied to trigger the lipid hyperaccumulation in an oleaginous Chlorella pyrenoidosa. Regular patterns of growth characteristics, lipid accumulation, physiological parameters, as well as the expression patterns of lipid biosynthesis-related genes were fully analyzed and compared. Results: Our results showed that all the nutrient stress conditions could enhance the lipid content considerably compared with the control. The total lipid and neutral lipid contents exhibit the most marked increment under nitrogen deficiency, achieving 50.32% and 34.29% of dry cell weight at the end of cultivation, respectively. Both photosynthesis indicators and reactive oxygen species parameters reveal that physiological stress turned up when exposed to nutrient depletions. Time-course transcript patterns of lipid biosynthesis-related genes showed that diverse expression dynamics probably contributes to the different lipidic phenotypes under stress conditions. By analyzing the correlation between lipid content and gene expression level, we pinpoint several genes viz. rbsL, me g6562, accA, accD, dgat g2354, dgat g3280 and dgat g7063, which encode corresponding enzymes or subunits of malic enzyme, ACCase and diacylglycerol acyltransferase in the de novo TAG biosynthesis pathway, are highly related to lipid accumulation and might be exploited as target genes for genetic modification. Conclusion: This study provided us not only a comprehensive picture of adaptive mechanisms from physiological perspective, but also a number of targeted genes that can be used for a systematic metabolic engineering. Besides, our results also represented the feasibility of lipid production through trophic transition cultivation modes, throwing light on a two-stage microalgal lipid production strategy with which heterotrophy stage provides sufficient robust seed and nitrogen-starvation photoautotrophy stage enhances the overall lipid productivity.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Figures
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION
Bligh, E. G., & Dyer, W. J. (1959) · Canadian Journal of Biochemistry and Physiology · doi:10.1139/o59-099 · Cited by 54,751
Reactive oxygen species: Metabolism, oxidative stress, and signal transduction
Apel, K., & Hirt, H. (2004) · Annual Review of Plant Biology · doi:10.1146/annurev.arplant.55.031903.141701 · Cited by 11,723
Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances
Hu, Q., Sommerfeld, M., Jarvis, E., Ghirardi, M., Posewitz, M., Seibert, M., & Darzins, A. (2008) · The Plant Journal · doi:10.1111/j.1365-313x.2008.03492.x · Cited by 3,745
Dual action of the active oxygen species during plant stress responses
Dat, J. F., Vandenabeele, S. I. J., Vranov�, E., MONTAGU, M. V., Inzé, D., & Breusegem, F. V. (2000) · Cellular and Molecular Life Sciences · doi:10.1007/s000180050041 · Cited by 1,909
An outlook on microalgal biofuels
Wijffels, R. H., & Barbosa, M. J. (2010) · Science · doi:10.1126/science.1189003 · Cited by 1,886
Protection against oxygen radicals: an important defence mechanism studied in transgenic plants
Foyer, C. H., Descourvières, P., & Kj, K. (1994) · Plant Cell & Environment · doi:10.1111/j.1365-3040.1994.tb00146.x · Cited by 1,256
Biodiesel from algae: challenges and prospects
Scott, S. A., Davey, M. P., Dennis, J. S., Horst, I., Howe, C. J., Lea-Smith, D. J., & Smith, A. G. (2010) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2010.03.005 · Cited by 1,183
Genetic Engineering of Algae for Enhanced Biofuel Production
Radakovits, R., Jinkerson, R. E., Darzins, A., & Posewitz, M. C. (2010) · Eukaryotic Cell · doi:10.1128/ec.00364-09 · Cited by 1,142
Xu, H., Miao, X., & Wu, Q. (2006) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2006.05.002 · Cited by 1,141
Li, Y., Horsman, M., Wang, B., Wu, N., & Lan, C. Q. (2008) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-008-1681-1 · Cited by 1,116
Self-citation
Research works printed but not linked: 1
- Chen J, Lang C, Hu Z, Liu Z, Huang R: Antisense PEP gene regulates to ratio of protein and lipid content in Brassica napus seeds. J Agric Biotechnol. 1998, 7: 316-320.
Cited by
Sorted by citations
Items 301–342 of 342
Microbial Lipid Technology Based on Oleaginous Microalgae
Zhang, L., Tsui, T., Tong, Y. W., Liu, R., & Aggarangsi, P. (2025) · Springer eBooks · doi:10.1007/978-981-96-0982-6_4 · Cited by 1
Zhang, L., Tsui, T., Tong, Y. W., Liu, R., & Rasmey, A.-H. M. (2025) · Springer eBooks · doi:10.1007/978-981-96-0982-6_9 · Cited by 1
McKeel, E., Kim, H., Jeon, S., McKinnon, B., Giraldo, J. P., & Klaper, R. D. (2025) · Frontiers in Plant Science · doi:10.3389/fpls.2025.1681466 · Cited by 1
Zhao, Y. V., Li, Z., Tu, X., & Xu, L. (2021) · IOP Conference Series Earth and Environmental Science · doi:10.1088/1755-1315/675/1/012040 · Cited by 1
Liu, C., Liu, Y., Yu, S., Dong, W., & Xue, Y. (2022) · Research Square · doi:10.21203/rs.3.rs-1557460/v1 · Cited by 1
Exploitation of microalgae for biotechnological applications
Xie, J. (2021) · doi:10.31274/td-20240329-321 · Cited by 1
Azaman, S. N. A. (2016) · Universiti Putra Malaysia Institutional Repository (Universiti Putra Malaysia)
Gene Expression Analysis in Dunaliella salina Under Salt Stress
Ganjali, S., Ahmadi, F. S., & Ahmadzadeh, H. (2019) · Journal of Phycological Research · doi:10.29252/jpr.3.1.287
Microalgae for combined nutrient recovery and biofuel production from sewage
Wood, N. J. (2020) · White Rose eTheses Online (University of Leeds, The University of Sheffield, University of York)
Gumbi, S. T., Kumar, A., & Olaniran, A. O. (2021) · Research Square · doi:10.21203/rs.3.rs-1070807/v1
Wu, P., Zhang, L., Chang, H., Xu, G., & Liu, M. (2021) · SSRN Electronic Journal · doi:10.2139/ssrn.3997855
Algal Oil as a Low‐Cost Feedstock for Biodiesel Production
Chhandama, M. V. L., Satyan, K. B., & Rokhum, S. L. (2022) · Wiley eBooks · doi:10.1002/9781119771364.ch4
Stress induced production of high-value organic compounds from freshwater microalgae
Thomas, J. P., Joseph, D., & Sureshkumar, P. (2020) · Algologia · doi:10.15407/alg30.03.306
Genetically Modified (GM) Microalgae for Biofuel Production
Bajpai, P. (2022) · SpringerBriefs in applied sciences and technology · doi:10.1007/978-981-19-2001-1_2
Lipids produced by microalgae and thraustochytrids
Colonia, B. S. O., Pereira, G. V. D. M., de Carvalho, J. C., Sousa, P. H. D. S., Fanka, L. S., Rodrigues, C., Medeiros, A. B. P., & Soccol, C. R. (2022) · Elsevier eBooks · doi:10.1016/b978-0-323-90631-9.00011-9
Adıgüzel, A. O. (2022) · Clean Energy Production Technologies · doi:10.1007/978-981-19-6806-8_2
Genetic engineering of algae material
Shailaja, V. L., John, C. M., & Kalaivani, M. (2023) · Elsevier eBooks · doi:10.1016/b978-0-443-18816-9.00012-5
Debroy, A., Nirmala, M. J., Pulimi, M., Peijnenburg, W. J. G. M., & Mukherjee, A. (2024) · SSRN Electronic Journal · doi:10.2139/ssrn.4789014
Xue, W., Qiu, Y., Feng, X., Zhang, Y., Xue, Y., & Liu, C. (2024) · SSRN Electronic Journal · doi:10.2139/ssrn.4805596
Alkane gene expression and its increased production in recombinant cyanobacteriaPreprint
Nagao, M., Ozaki, T., Fukuda, H., Kanesaki, Y., & Asayama, M. (2024) · Research Square · doi:10.21203/rs.3.rs-4404191/v1
Wu, H., Qiu, Y., Feng, X., Zhang, Y., Xue, Y., Xue, W., & Liu, C. B. (2024) · Research Square · doi:10.21203/rs.3.rs-4778598/v1
Microalgae for biogas production
Álvaro, A. G., Palomar, C. R., Ibrahím, F. G. G., Mínguez, D. R., & de Godos, I. (2025) · Elsevier eBooks · doi:10.1016/b978-0-443-33679-9.00012-6 · Cited by 1
Nayar, S. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2025.12.15.690641
Zhang, X., Zhang, Z., Peng, Y., Zhang, Y., Li, Q., & Sun, D. (2024) · Figshare · doi:10.6084/m9.figshare.26660152
Gao, B., Hong, J., Chen, J., Zhang, H., Hu, R., & Zhang, C. (2024) · Figshare · doi:10.6084/m9.figshare.26562913
Zhang, X., Zhang, Z., Peng, Y., Zhang, Y., Li, Q., & Sun, D. (2024) · Figshare · doi:10.6084/m9.figshare.26660155
Lucato, V., Mazzola, G., Gao, F., Abiusi, F., Badocco, D., Pastore, P., Mathys, A., & Sforza, E. (2026) · Biotechnology for Biofuels and Bioproducts · doi:10.1186/s13068-026-02743-9
Coulombier, N. (2020) · theses.fr (ABES) · doi:10.70675/1fc6442az8116z418dza169z7fa855798c1c
Biological valorization of non-food feedstocks: A critical review
Guo, Q., Wei, Y., Wang, F., Zhao, S., Song, G., Zhao, K., & Liu, R. (2026) · Biotechnology Advances · doi:10.1016/j.biotechadv.2026.108918
Microalgae-based biorefineries
Behera, D., & Saady, N. M. C. (2026) · Elsevier eBooks · doi:10.1016/b978-0-443-34137-3.00006-6
Yadav, A., Singh, L. A., Sharma, S., Bhardwaj, R., Kumari, P., Swapnil, P., & Meena, M. (2026) · Scientific Reports · doi:10.1038/s41598-026-57838-y
Ding, Z.-R., Wu, X.-M., Li, P., Qian, X.-Y., Liu, C. Y., Yin, M.-H., Qu, C.-F., Cao, Z., Jung, J., Liu, L. L., et al. (2026) · Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology · doi:10.1016/j.cbpc.2026.110605
Han, B., Chen, H., Qi, X., Ji, N., Pan, Y., Cheng, X., Zhou, L., & Shen, X. (2026) · Harmful Algae · doi:10.1016/j.hal.2026.103185
Proteomic response of Gymnodinium catenatum to ambient nitrogen and phosphorus changes
Kong, L., Zhang, Y., Li, C., He, Y., & Gui, H. (2026) · Frontiers in Marine Science · doi:10.3389/fmars.2026.1950162
Bharathiraja, B., Flora, R. M. N., Raj, P. V. A., Praveenkumar, R., & Sivarathnakumar, S. (2026) · Journal of the Indian Chemical Society · doi:10.1016/j.jics.2026.103148
李会雪, Fu, R., Zdarta, J., Cui, J., Campos, L. C., Sun, L., & Ren, Z. (2026) · Separation and Purification Technology · doi:10.1016/j.seppur.2026.140630
Metadata
- Authors
- Fan, J., Cui, Y., Wan, M., Wang, W., & Li, Y.
- Affiliations
- East China University of Science and Technology (CN)
- Corresponding author
- Wang, Weiliang
- Li, Yuanguang
- Venue
- Biotechnology for Biofuels
- Published
- 2014-01-30
- Volume
- 7
- Issue
- 1
- Pages
- 17-17
- ISSN
- 1754-6834 (ISSN-L)
- PMC
- PMC3916312
- OpenAlex
- W2103978564
- Cited by
- 341
- References
- 54
- Citations by year
- 2026: 11 · 2025: 21 · 2024: 19 · 2023: 14 · 2022: 38 · 2021: 40 · 2020: 30 · 2019: 44 · 2018: 37 · 2017: 40 · 2016: 22 · 2015: 19 · 2014: 6
- FWCI
- 9.57
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- Biotechnology for Biofuels (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (31300295, 2060204)
- China Postdoctoral Science Foundation
- State Key Laboratory of Bioreactor Engineering (2060204)
- National Key Research and Development Program of China (2060204, 2011CB200904)
- Fundamental Research Funds for the Central Universities (2060204)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- Chlorella pyrenoidosa, lipid accumulation, lipid biosynthesis genes, nitrogen deficiency, phosphorus deficiency, iron deficiency, nutritional stress, transcriptome, triglyceride synthesis, malic enzyme, acetyl-CoA carboxylase, diacylglycerol acyltransferase, reactive oxygen species, microalgal biofuels, metabolic engineering, heterotrophic cultivation, two-stage cultivation
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
