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Cited by 103Mosquito-borne diseases and controlDiptera species taxonomy and behaviorMalaria Research and Control

The DOI matches its Crossref record; 54 of the 55 references it cites were found in a registry or an index.

Feeding patterns of molestus and pipiens forms of Culex pipiens (Diptera: Culicidae) in a region of high hybridization

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Gomes, Bruno, Sousa, Carla A, Vicente, José L, Pinho, Leonor, Calderón, Isabel, Arez, Eliane, Almeida, António PG, Donnelly, Martin J, Pinto, João·2013·Parasites & Vectors
DOI 10.1186/1756-3305-6-93PMC PMC3637809
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Abstract

BACKGROUND: Two biological forms of the mosquito Culex pipiens s.s., denoted pipiens and molestus, display behavioural differences that may affect their role as vectors of arboviruses. In this study, the feeding patterns of molestus and pipiens forms were investigated in Comporta (Portugal), where high levels of inter-form admixture have been recorded. METHODS: Indoor and outdoor mosquito collections were performed in the summer of 2010. Collected Cx. pipiens s.l. females were molecularly identified to species and form by PCR and genotyped for six microsatellites. The source of the blood meal in post-fed females was determined by ELISA and mitochondrial DNA sequencing. RESULTS: The distribution of the forms differed according to the collection method. The molestus form was present only in indoor collections, whereas pipiens and admixed individuals were sampled both indoors and outdoors. In both forms, over 90% of blood meals were made on avian hosts. These included blood meals taken from Passeriformes (Passer domesticus and Turdus merula) by females caught resting inside domestic shelters. CONCLUSION: Genetic structure and blood meal analyses suggest the presence of a bird biting molestus population in the study area. Both forms were found to rest indoors, mainly in avian shelters, but at least a proportion of females of the pipiens form may bite outdoors in sylvan habitats and then search for anthropogenic resting sites to complete their gonotrophic cycle. This behaviour may potentiate the accidental transmission of arboviruses to humans in the region.

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Crossref · 10.1186/1756-3305-6-93

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Figure 1 Bayesian cluster analysis of Cx. pipiens s.s. mosquitoes conducted by STRUCTURE in Comporta (2010). A: Individuals sorted by their ancestral probability; B: Individuals sorted by collection method and ancestral probability; IR: indoor resting inside shelters; CDC-G: CDC light traps in ground level; CDC-C: CDC light traps in canopy of trees; HLC: human landing catches; a: admixed individuals (0.1 < Tq < 0.9). Columns correspond to the multilocus genotype of each individual, partitioned in different colours representing the probability of ancestry (qi) to each cluster (Red: molestus; Blue: pipiens). Individuals were ordered according to their geographic information. Lines indicate the qi threshold used to determine admixed individuals (see Methods).
Figure 1 Bayesian cluster analysis of Cx. pipiens s.s. mosquitoes conducted by STRUCTURE in Comporta (2010). A: Individuals sorted by their ancestral probability; B: Individuals sorted by collection method and ancestral probability; IR: indoor resting inside shelters; CDC-G: CDC light traps in ground level; CDC-C: CDC light traps in canopy of trees; HLC: human landing catches; a: admixed individuals (0.1 < Tq < 0.9). Columns correspond to the multilocus genotype of each individual, partitioned in different colours representing the probability of ancestry (qi) to each cluster (Red: molestus; Blue: pipiens). Individuals were ordered according to their geographic information. Lines indicate the qi threshold used to determine admixed individuals (see Methods).CC BY · PubMed Central
Figure 2 Frequency of the groups defined by STRUCTURE by locality. Black dot: positive sample site for Cx. pipiens s.s. (Cambado, Comporta, Pego, Possanco, Torre); Red dot: negative sample site for Cx. pipiens s.s. (Carvalhal, Monte Novo do Sul). Color graphics: proportion of females; Red: cluster 1 (molestus form); Blue: cluster 2 (pipiens form); Purple: admixed (hybrids). Grey-scale graphics: proportion of mosquito collections; Dark grey: proportion of collection performed in chicken coops; Light grey: proportion of collection performed in other type of shelter.
Figure 2 Frequency of the groups defined by STRUCTURE by locality. Black dot: positive sample site for Cx. pipiens s.s. (Cambado, Comporta, Pego, Possanco, Torre); Red dot: negative sample site for Cx. pipiens s.s. (Carvalhal, Monte Novo do Sul). Color graphics: proportion of females; Red: cluster 1 (molestus form); Blue: cluster 2 (pipiens form); Purple: admixed (hybrids). Grey-scale graphics: proportion of mosquito collections; Dark grey: proportion of collection performed in chicken coops; Light grey: proportion of collection performed in other type of shelter.CC BY · PubMed Central

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Citations

Cites

Research works printed but not linked: 6

  1. Ribeiro H, Ramos HC: Identification keys of the mosquitoes (Diptera: Culicidae) of Continental Portugal, Acores and Madeira. Eur Mosq Bull. 1999, 3: 1-11.
  2. Simões MJ, Próspero MI, Henrique R: Optimização da técnica ELISA “two sites” utilizada na identificação de refeições sanguíneas de mosquitos. Rev Port Doenc Infec. 1995, 18: 225-229.
  3. World Health Organization: Manual on Practical Entomology in Malaria. Part II: Methods and Techniques Geneva. 1975, Geneva, 191-
  4. Ribeiro H, Pires CA, Ramos HC, Capela RA: Research on the mosquitoes of Portugal (Diptera, Culicicae). VIII- On the occurrence of Culex (Culex) molestus Forskål, 1775. J Soc Cienc Med Lisb. 1983, 147: 185-188.
  5. Sousa CA:Malaria vectorial capacity and competence ofAnopheles atroparvusVan Thiel, 1927 (Diptera, Culicidae): Implications for the potential re-emergence of malaria in Portugal.PhD thesis. 2008, Universidade Nova de Lisboa, Instituto de Higiene e Medicina Tropical,
  6. Formosinho P, Santos-Silva MM, Santos A, Melo P, Encarnação V, Santos N, Nunes T, Agrícola R, Portas M: O vírus West Nile em Portugal – estudos de vigilância epidemiológica. Rev Port. Cienc Vet. 2006, 101: 61-68.

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Metadata

Authors
Gomes, B., Sousa, C. A., Vicente, J. L., Pinho, L., Calderón, I., Arez, E., Almeida, A. P., Donnelly, M. J., & Pinto, J.
Affiliations
  • Universidade Nova de Lisboa (PT)
  • Liverpool School of Tropical Medicine (GB)
Corresponding author
Pinto, João
ORCID
Venue
Parasites & Vectors
Publisher
Springer Science and Business Media LLC
Published
2013-04-11
Volume
6
Issue
1
Pages
93-93
ISSN
1756-3305 (ISSN-L)
DOI
10.1186/1756-3305-6-93
PMC
PMC3637809
OpenAlex
W2146195042
Cited by
103
References
55
Citations by year
2026: 7 · 2025: 5 · 2024: 9 · 2023: 8 · 2022: 7 · 2021: 11 · 2020: 10 · 2019: 7 · 2018: 8 · 2017: 10 · 2016: 10 · 2015: 8 · 2014: 3
FWCI
7.14
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Parasites & Vectors (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • LSTM Online Archive (Liverpool School of Tropical Medicine) (acceptedVersion)
  • Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion, other-oa)
Funders
Fundação para a Ciência e a Tecnologia (SFRH/BD/36410/2007, POCI/BIA-BDE/57650/2004)
Topics
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Mosquito-borne diseases and control
  • Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Diptera species taxonomy and behavior
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Malaria Research and Control
Keywords
Culex pipiens, blood meal sources, avian hosts, Passer domesticus, Turdus merula, indoor biting, hybridization, arbovirus vectors
MeSH terms
Animals, Birds, Blood Cells, Culex, Feeding Behavior, Female, Genotype, Insect Vectors, Portugal, Polymerase Chain Reaction, Microsatellite Repeats

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