Organ Preservation Using a Photosynthetic Solution
Open accessAbstract
BACKGROUND: Organs harvested from a body lapsing into circulatory deficit are exposed to low O2/high CO2, and reach a critical point where original functionality after transplantation is unlikely. The present study evaluates the effect of respiratory assistance using Chlorella photosynthesis on preservation of the rat pancreas from the viewpoint of donation after cardiac death (DCD). METHODS: Gas was exchanged through the peritoneum of rats under controlled ventilation with or without Chlorella photosynthetic respiratory assistance. A gas permeable pouch containing Chlorella in solution was placed in the peritoneum and then the space between the pouch and the peritoneum was filled with an emulsified perfluorocarbon gas carrier. Rat DCD pancreases procured 3 h after cardiac arrest were preserved for 30 min in a cold or mildly hypothermic environment or in a mildly hypothermic environment with photosynthetic respiratory support. The pancreases were then heterotopically transplanted into rats with STZ-induced diabetes. RESULTS: Levels of blood oxygen (PaO2) and carbon dioxide (PaCO2) increased and significantly decreased, respectively, in rats with mechanically reduced ventilation and rats given intraperitoneal photosynthetic respiratory support when compared with those without such support. Transplantation with DCD pancreases that had been stored under photosynthetic respiratory support resulted in the survival of all rats, which is impossible to achieve using pancreases that have been maintained statically in cold storage. CONCLUSION: Respiratory assistance using photosynthesis helps to improve not only blood gas status in the event of respiratory insufficiency, but also graft recovery after pancreas transplantation with a DCD pancreas that has been damaged by prolonged warm ischemia.
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A respiratory support system using C. vulgaris to provide a constant supply of O2 extended the viability of rat pancreas cells after cardiac death for transplantation.
“A respiratory support system, leveraging the ability of C. vulgaris to provide a constant supply of O2, was developed to extend the viability of rat pancreas cells after cardiac death for transplantation purposes [121].”
From Emerging Applications of Chlorella sp. and Spirulina (Arthrospira) sp. (2023) · cites this paper as [121]
The Methods and Figure 3 description identify Chlorella vulgaris as the oxygen-producing alga and describe 30 minutes of photosynthetic support for rat pancreases retrieved three hours after cardiac arrest. The Abstract Results report survival after transplantation of supported pancreases, unlike those kept in static cold storage. The study transplanted whole pancreases, not isolated pancreas cells, and does not establish a constant oxygen supply beyond the treatment period. (LLM, conf=0.86)
Experimental — accuracy not yet measuredTransplantation of rat pancreas cells preserved under photosynthetic respiratory support resulted in survival of all treated rats, an outcome unattainable with static cold storage.
“The transplantation of these rat cells, which were preserved under photosynthetic respiratory support, resulted in the survival of all treated rats—an outcome that was unattainable when using pancreases stored in static cold conditions.”
From Emerging Applications of Chlorella sp. and Spirulina (Arthrospira) sp. (2023) · cites this paper as [121]
The Abstract (Results) and Results and discussion report that all rats receiving photosynthesis-supported pancreases survived, whereas five of six receiving cold-stored pancreases died within hours. However, the study transplanted whole rat pancreases, not isolated pancreas cells as the claim states. (LLM, conf=0.88)
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- Authors
- Yamaoka, I., Kikuchi, T., Arata, T., & Kobayashi, E.
- Affiliations
- Otsuka (Japan) (JP)
- Corresponding author
- Kobayashi, Eiji
- Venue
- Transplantation Research
- Published
- 2012-04-24
- Volume
- 1
- Issue
- 1
- Pages
- 2-2
- ISSN
- 2047-1440 (ISSN-L)
- PMC
- PMC3552571
- OpenAlex
- W2116156789
- Cited by
- 24
- References
- 25
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 4 · 2023: 4 · 2022: 4 · 2021: 3 · 2020: 1 · 2019: 1 · 2016: 1 · 2015: 1
- FWCI
- 0.33
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by
- Locations
- Transplantation Research (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Surgery › Organ Transplantation Techniques and Outcomes
- Health Sciences › Medicine › Surgery › Transplantation: Methods and Outcomes
- Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
- Keywords
- organ preservation, Chlorella, donation after circulatory death, pancreas transplantation, cold storage, warm ischemia, hypothermic storage, respiratory failure, rat model
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