Photosynthetic Microorganisms for the Oxygenation of Advanced 3D Bioprinted Tissues
Abstract
3D bioprinting technology has emerged as a tool that promises to revolutionize the biomedical field, including tissue engineering and regeneration. Despite major technological advancements, several challenges remain to be solved before 3D bioprinted tissues could be fully translated from the bench to the bedside. As oxygen plays a key role in aerobic metabolism, which allows energy production in the mitochondria; as a consequence, the lack of tissue oxygenation is one of the main limitations of current bioprinted tissues and organs. In order to improve tissue oxygenation, recent approaches have been established for a broad range of clinical applications, with some already applied using 3D bioprinting technologies. Among them, the incorporation of photosynthetic microorganisms, such as microalgae and cyanobacteria, is a promising approach that has been recently explored to generate chimerical plant-animal tissues where, upon light exposure, oxygen can be produced and released in a localized and controlled manner. This review will briefly summarize the state-of-the-art approaches to improve tissue oxygenation, as well as studies describing the use of photosynthetic microorganisms in 3D bioprinting technologies. STATEMENT OF SIGNIFICANCE: 3D bioprinting technology has emerged as a tool for the generation of viable and functional tissues for direct in vitro and in vivo applications, including disease modeling, drug discovery and regenerative medicine. Despite the latest advancements in this field, suboptimal oxygen delivery to cells before, during and after the bioprinting process limits their viability within 3D bioprinted tissues. This review article first highlights state-of-the-art approaches used to improve oxygen delivery in bioengineered tissues to overcome this challenge. Then, it focuses on the emerging roles played by photosynthetic organisms as novel biomaterials for bioink generation. Finally, it provides considerations around current challenges and novel potential opportunities for their use in bioinks, by comparing latest published studies using algae for 3D bioprinting.
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3D bioprinted living scaffolds incorporating microalgae can produce O2, adapt to irregular-shaped wounds, and promote healing.
“Another strategy that has been developed to provide O2 to the tissues is 3D bioprinting, incorporating microalgae to create “living” scaffolds that can produce O2.”
From Emerging Applications of Chlorella sp. and Spirulina (Arthrospira) sp. (2023) · cites this paper as [125]
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Emerging Applications of Chlorella sp. and Spirulina (Arthrospira) sp.
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Rasheed, A. S., Thajuddin, F., Elumalai, A., Thajuddin, S. F., Nooruddin, T., & Dharumadurai, D. (2026) · Biotechnology and Bioprocess Engineering · doi:10.1007/s12257-026-00285-3
- Authors
- Ortega, J. S., Corrales-Orovio, R., Ralph, P., Egaña, J. T., & Gentile, C.
- Affiliations
- University of Technology Sydney (AU)
- Pontificia Universidad Católica de Chile (CL)
- Corresponding author
- Egaña, José Tomás
- Gentile, Carmine
- Venue
- Acta Biomaterialia
- Publisher
- Elsevier BV
- Published
- 2023-07
- Volume
- 165
- Pages
- 180-196
- ISSN
- 1742-7061 (ISSN-L)
- OpenAlex
- W4280644971
- Cited by
- 47
- References
- 133
- Citations by year
- 2026: 11 · 2025: 17 · 2024: 9 · 2023: 7 · 2022: 3
- FWCI
- 0.80
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Acta Biomaterialia (publishedVersion)
- PubMed (publishedVersion)
- Funders
- University of Technology Sydney
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Engineering › Automotive Engineering › Additive Manufacturing and 3D Printing Technologies
- Keywords
- 3D bioprinting, tissue oxygenation, photosynthetic microorganisms, microalgae, cyanobacteria, bioinks, oxygen delivery, tissue engineering, regenerative medicine
- MeSH terms
- Animals, Biocompatible Materials, Tissue Engineering, Regenerative Medicine, Tissue Scaffolds, Bioprinting, Printing, Three-Dimensional
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