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Cited by 19Parkinson's Disease Mechanisms and TreatmentsMedicinal Plants and NeuroprotectionNuclear Receptors and Signaling

RETRACTED ARTICLE: Asiaticoside, a trisaccaride triterpene induces biochemical and molecular variations in brain of mice with parkinsonism

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Sampath, Uvarajan, Janardhanam, Vanisree Arambakkam·2013·Translational Neurodegeneration
DOI 10.1186/2047-9158-2-23PMC PMC4177538
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Abstract

Background: Parkinson’s disease characterized by oxidative stress and mitochondrial damage in the pars compacta of substantia nigra remains a challenge to manage with an added disadvantage of side effects of L-levo dopa, the standard drug used for therapy. Thus, an alternative approach of utilizing natural components would be beneficial in the management of the disease. The present study was aimed to investigate the potential role of asiaticoside (As), a trisaccaride triterpene against1 – methyl 4 – phenyl 1,2,3,6 tetrahydropyridine (MPTP)-induced neurotoxicity in experimental mice. Methods: Mice were divided into 4 groups: Group I received vehicle saline, group II was treated with 20 mg/kg of body weight of MPTP (2 doses with 2 h intervals), group III received MPTP along with 50 mg/kg body weight of As for the 21 consecutive days starting from the day of MPTP intoxication. Group IV received 50 mg/kg body weight of asiaticoside for the same period serving as drug control. Animals were sacrificed at the end of experimental period and the striatum and midbrain samples were analyzed for enzyme assays, transmission electron microscopic (TEM) analysis. Immunofluorescent assay was performed to study the expression of GFAP to detect astrocyte, which are activated due to neuronal damage. Imunohistochemical studies were carried out to quantify the expression of Bax and Bcl2, the molecular signatures that would provide clues of the extent of neurodegeneration. Results: The activities of enzymes were increased on As administration when compared with those of group II animals. Expressions of Bax and Bcl2 along with GFAP did show significant variations (p < 0.05) on MPTP treatment when compared to control animals and the changes were found to be reversed significantly (p < 0.05) after treatment with asiaticoside. TEM analysis also showed attenuated degenerative architecture on As administration. The mice which received As alone (drug control IV) did not show significant variation from that of the control mice. Conclusion: The observations suggest that asiaticoside may be efficacious in protecting neurons from the oxidative damage caused by the insult of MPTP.

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  • Asiaticoside prevents oxidative damage and protects against Parkinson's toxicity in MPTP-treated mice by blocking Bax-mediated neuronal death and promoting Bcl-2 expression.

    “Similar mechanism of action had been observed in an earlier study using MPTP‐treated mice where asiaticoside administration prevented oxidative damage to the neurons and protected against Parkinson's toxicity specifically by blocking Bax‐mediated neuronal death and promoting Bcl‐2 expression. 64”

    From Therapeutic Properties and Pharmacological Activities of Asiaticoside and Madecassoside: A Review (2023) · cites this paper as [64]

    The Abstract Results and Discussion report that asiaticoside-treated, MPTP-exposed mice showed reduced Bax expression, increased Bcl-2 expression, and signs of neuroprotection. But the Discussion calls the mechanism “yet-to-identify,” so the claim that asiaticoside protected neurons specifically by blocking Bax-mediated death and promoting Bcl-2 states a causal mechanism more firmly than the paper does. Page numbers are not provided in our copy. (LLM, conf=0.88)

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Authors
Sampath, U., & Janardhanam, V. A.
Affiliations
University of Madras (IN)
Corresponding author
Janardhanam, Vanisree Arambakkam
Venue
Translational Neurodegeneration
Publisher
Springer Science and Business Media LLC
Published
2013-11-21
Volume
2
Issue
1
Pages
23-23
ISSN
2047-9158 (ISSN-L)
DOI
10.1186/2047-9158-2-23
PMC
PMC4177538
OpenAlex
W2098774394
Cited by
19
References
0
Citations by year
2024: 2 · 2023: 3 · 2022: 2 · 2021: 2 · 2020: 3 · 2019: 2 · 2017: 1 · 2015: 4
FWCI
0.85
Type
journal article
Language
en
Status
Retracted
Update notices
retraction (2024-06-05)
Open access
Licence
Locations
  • Translational Neurodegeneration (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Topics
  • Health Sciences › Medicine › Neurology › Parkinson's Disease Mechanisms and Treatments
  • Health Sciences › Medicine › Complementary and alternative medicine › Medicinal Plants and Neuroprotection
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nuclear Receptors and Signaling
Keywords
asiaticoside, Parkinson's disease, MPTP-induced neurotoxicity, neuroprotection, oxidative stress, substantia nigra pars compacta, Bax/Bcl-2 expression, striatum, mitochondrial damage, retracted article

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