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Cited by 137Milk Quality and Mastitis in Dairy CowsAnimal health and immunologyMicrobial infections and disease research

Acute Phase Response in Dairy Cows with Experimentally Induced Escherichia coli Mastitis

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Hirvonen, J., Eklund, K., Teppo, A. M., Huszenicza, G., Kulcsar, M., Saloniemi, H., Pyörälä, S.·1999·Acta Veterinaria Scandinavica
DOI 10.1186/bf03547039PMC PMC8043231
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Abstract

Six Finnish Ayrshire cows were challenged intramammarily with 1500 CFU of Escherichia coli (E. coli) into single udder quarters, and the challenge was repeated into contralateral quarters 3 weeks later. All cows received flunixine meglumine once, and 3 of them were also treated with enrofloxacin. At the 2nd challenge, treatments were changed vice versa. The development of mastitis was followed by monitoring of systemic and local clinical signs, and with serial milk and serum samples. Intramammary challenge with E. coli produced clinical mastitis in all cows, the severity of the disease varying greatly between the animals. No significant changes between the 2 treatment regimens or sequent challenges were found for any of the clinical parameters. The response of each cow followed the same pattern after both challenges; three of the cows became mildly and the other 3 either moderately or severely affected. Two severely affected cows had to be euthanized because of severe mastitis. Serum haptoglobin and amyloid-A concentrations peaked 2-3 days after bacterial challenge. Serum haptoglobin did not correlate with the severity of the disease. Serum amyloid-A rose gradually in the severely affected cows, and significant differences were found between severely versus moderately or mildly affected cows at day 4. Serum tumor necrosis factor alpha concentrations increased only in the severely affected cows. Serum cortisol response was prolonged in the severely diseased animals, and was significantly lower after the second challenge. Serum nitrite/nitrate concentration increased in the severely affected cows. This indicated excess nitric oxide production during acute E. coli mastitis. Strongly decreased milk production, and high bacterial growth in the infected quarters were best predictors for the outcome from acute E. coli mastitis.

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Crossref · 10.1186/bf03547039

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  • Damage to the host from coliform mastitis is mediated by the release of endotoxin.

    “Damage to the host is mediated by the release of endotoxin.”

    From Coliform mastitis (2003) · cites this paper as [21]

  • Decreased milk production during clinical coliform mastitis results both directly and indirectly from the local and systemic effects of endotoxin.

    “Decreased milk production during clinical coliform mastitis results both directly and indirectly from the local and systemic effects of endotoxin [3, 21, 22].”

    From Coliform mastitis (2003) · cites this paper as [21]

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Authors
Hirvonen, J., Eklund, K., Teppo, A. M., Huszenicza, G., Kulcsar, M., Saloniemi, H., & Pyörälä, S.
Affiliations
  • University of Helsinki (FI)
  • Helsinki University Hospital (FI)
  • University of Veterinary Medicine (HU)
Corresponding author
Pyörälä, S.
Venue
Acta Veterinaria Scandinavica
Publisher
Springer Science and Business Media LLC
Published
1999-03
Volume
40
Issue
1
Pages
35-46
ISSN
  • 0044-605X (ISSN-L)
  • 1751-0147
DOI
10.1186/bf03547039
PMC
PMC8043231
OpenAlex
W164586581
Cited by
137
References
41
Citations by year
2026: 1 · 2025: 1 · 2024: 4 · 2023: 2 · 2021: 3 · 2020: 6 · 2019: 2 · 2018: 3 · 2017: 4 · 2016: 6 · 2015: 6 · 2014: 5 · 2013: 7 · 2012: 7
FWCI
7.66
Type
journal article
Language
en
Open access
Licence
Locations
  • Acta veterinaria Scandinavica (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Milk Quality and Mastitis in Dairy Cows
  • Health Sciences › Veterinary › Small Animals › Animal health and immunology
  • Life Sciences › Immunology and Microbiology › Microbiology › Microbial infections and disease research
Keywords
acute phase response, bovine mastitis, Escherichia coli mastitis, dairy cattle, enrofloxacin, haptoglobin, serum amyloid A, TNF-α, cortisol, nitric oxide, nitrite/nitrate, milk production, disease severity, Ayrshire cattle
MeSH terms
Enrofloxacin, Acute-Phase Reaction, Serum Amyloid A Protein, Animals, Anti-Infective Agents, Anti-Inflammatory Agents, Non-Steroidal, Cattle, Clonixin, Escherichia coli Infections, Female, Haptoglobins, Hydrocortisone, Mastitis, Bovine, Milk, Nitrites, Random Allocation, Tumor Necrosis Factor-alpha, Colony Count, Microbial, Quinolones, Fluoroquinolones

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