Cited by 547Gene expression and cancer classificationBioinformatics and Genomic NetworksBreast Cancer Treatment Studies

'Gene shaving' as a method for identifying distinct sets of genes with similar expression patterns

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Hastie, Trevor, Tibshirani, Robert, Eisen, Michael B, Alizadeh, Ash, Levy, Ronald, Staudt, Louis, Chan, Wing C, Botstein, David +1 more·2000·Genome Biology

Abstract

BACKGROUND: Large gene expression studies, such as those conducted using DNA arrays, often provide millions of different pieces of data. To address the problem of analyzing such data, we describe a statistical method, which we have called 'gene shaving'. The method identifies subsets of genes with coherent expression patterns and large variation across conditions. Gene shaving differs from hierarchical clustering and other widely used methods for analyzing gene expression studies in that genes may belong to more than one cluster, and the clustering may be supervised by an outcome measure. The technique can be 'unsupervised', that is, the genes and samples are treated as unlabeled, or partially or fully supervised by using known properties of the genes or samples to assist in finding meaningful groupings. RESULTS: We illustrate the use of the gene shaving method to analyze gene expression measurements made on samples from patients with diffuse large B-cell lymphoma. The method identifies a small cluster of genes whose expression is highly predictive of survival. CONCLUSIONS: The gene shaving method is a potentially useful tool for exploration of gene expression data and identification of interesting clusters of genes worth further investigation.

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Authors
Hastie, T., Tibshirani, R., Eisen, M. B., Alizadeh, A., Levy, R., Staudt, L., Chan, W. C., Botstein, D., & Brown, P.
Affiliations
  • Stanford University (US)
  • Lawrence Berkeley National Laboratory (US)
  • Stanford Medicine (US)
  • National Institutes of Health (US)
  • University of Nebraska Medical Center (US)
Corresponding author
Tibshirani, Robert
Venue
Genome Biology
Published
2000-08-04
Volume
1
Issue
2
Pages
RESEARCH0003-RESEARCH0003
ISSN
  • 1465-6906 (ISSN-L)
  • 1474-760X
OpenAlex
W1774711529
Cited by
547
References
18
Citations by year
2025: 1 · 2024: 8 · 2022: 7 · 2021: 7 · 2020: 5 · 2019: 11 · 2018: 12 · 2017: 11 · 2016: 17 · 2015: 20 · 2014: 20 · 2013: 21 · 2012: 18
FWCI
11.82
Type
journal article
Language
en
Open access
Locations
  • Genome biology (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • Lincoln (University of Nebraska) (submittedVersion)
Funders
  • National Science Foundation
  • National Institutes of Health
  • National Cancer Institute (U01CA084967, P01CA034233, CA 34233, UOI-CA 84967)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene expression and cancer classification
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bioinformatics and Genomic Networks
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Breast Cancer Treatment Studies
Keywords
gene expression clustering, gene expression analysis, supervised clustering, hierarchical clustering, diffuse large B-cell lymphoma, survival prediction, gene expression signatures, microarray data
MeSH terms
Algorithms, Humans, RNA, Messenger, Gene Expression Regulation, Neoplastic, Cluster Analysis, Survival Analysis, Lymphoma, B-Cell, Lymphoma, Large B-Cell, Diffuse, Computational Biology, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling

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