The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Application of phage display to high throughput antibody generation and characterization
Open accessAbstract
We have created a high quality phage display library containing over 10 10 human antibodies and describe its use in the generation of antibodies on an unprecedented scale. We have selected, screened and sequenced over 38,000 recombinant antibodies to 292 antigens, yielding over 7,200 unique clones. 4,400 antibodies were characterized by specificity testing and detailed sequence analysis and the data/clones are available online. Sensitive detection was demonstrated in a bead based flow cytometry assay. Furthermore, positive staining by immunohistochemistry on tissue microarrays was found for 37% (143/381) of antibodies. Thus, we have demonstrated the potential of and illuminated the issues associated with genome-wide monoclonal antibody generation.
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Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture
Ying, Q.-L., Stavridis, M., Griffiths, D., Li, M., & Smith, A. (2003) · Nature Biotechnology · doi:10.1038/nbt780 · Cited by 1,504
p75 and Trk: A two-receptor system
Chao, M. V., & Hempstead, B. L. (1995) · Trends in Neurosciences · doi:10.1016/0166-2236(95)93922-k · Cited by 857
Bach, H., Mazor, Y., Shaky, S., Shoham-Lev, A., Berdichevsky, Y., Gutnick, D. L., & Benhar, I. (2001) · Journal of Molecular Biology · doi:10.1006/jmbi.2001.4914 · Cited by 178
Cited by
A roadmap to generate renewable protein binders to the human proteome
Renewable Protein Binder Working Group, Colwill, K., & Gräslund, S. (2011) · Nature Methods · doi:10.1038/nmeth.1607 · Cited by 374
Phage display-derived human antibodies in clinical development and therapy
Frenzel, A., Schirrmann, T., & Hust, M. (2016) · mAbs · doi:10.1080/19420862.2016.1212149 · Cited by 346
Applications of single-chain variable fragment antibodies in therapeutics and diagnostics
Weisser, N. E., & Hall, J. C. (2009) · Biotechnology Advances · doi:10.1016/j.biotechadv.2009.04.004 · Cited by 261
Animal‐free alternatives and the antibody iceberg
Gray, A., Bradbury, A. R. M., Knappik, A., Plückthun, A., Borrebaeck, C. A. K., & Dübel, S. (2020) · Nature Biotechnology · doi:10.1038/s41587-020-0687-9 · Cited by 111
Tulika, T., Pedersen, R. W., Rimbault, C., Ahmadi, S., Rivera‐de‐Torre, E., Fernández‐Quintero, M. L., Loeffler, J. R., Bohn, M., Ljungars, A., Ledsgaard, L., et al. (2023) · Protein Science · doi:10.1002/pro.4821 · Cited by 11
Metadata
- Authors
- Schofield, D. J., Pope, A. R., Clementel, V., Buckell, J., Chapple, S. D., Clarke, K. F., Conquer, J. S., Crofts, A. M., Crowther, S. R., Dyson, M. R., Flack, G., Griffin, G. J., Hooks, Y., Howat, W. J., Kolb-Kokocinski, A., Kunze, S., Martin, C. D., Maslen, G. L., Mitchell, J. N., O'Sullivan, M., Perera, R. L., Roake, W., Shadbolt, S. P., Vincent, K. J., Warford, A., Wilson, W. E., Xie, J., Young, J. L., & McCafferty, J.
- Venue
- Genome Biology
- Published
- 2007-11-29
- Volume
- 8
- Issue
- 11
- Pages
- R254-R254
- ISSN
- 1465-6906 (ISSN-L)
- 1474-760X
- OpenAlex
- W2152996957
- Cited by
- 236
- References
- 3
- FWCI
- 8.66
- Type
- journal article
- Language
- en
- Open access
- gold
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- The paper
- Schofield, D. J., Pope, A. R., Clementel, V., Buckell, J., Chapple, S. D., Clarke, K. F., Conquer, J. S., Crofts, A. M., Crowther, S. R., Dyson, M. R., Flack, G., Griffin, G. J., Hooks, Y., Howat, W. J., Kolb-Kokocinski, A., Kunze, S., Martin, C. D., Maslen, G. L., Mitchell, J. N., . . . McCafferty, J. (2007). Application of phage display to high throughput antibody generation and characterization. Genome Biology. https://doi.org/10.1186/gb-2007-8-11-r254
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
