The DOI matches its Crossref record; 104 of the 105 references it cites were found in a registry or an index.
Proteomic analysis of human follicular fluid from fertile women
Open accessAbstract
BACKGROUND: Follicular fluid is a unique biological fluid in which the critical events of oocyte and follicular maturation and somatic cell-germ cell communication occur. Because of the intimate proximity of follicular fluid to the maturing oocyte, this fluid provides a unique window into the processes occurring during follicular maturation. A thorough identification of the specific components within follicular fluid may provide a better understanding of intrafollicular signaling, as well as reveal potential biomarkers of oocyte health for women undergoing assisted reproductive treatment. In this study, we used high and low pH HPLC peptide separations followed by mass spectrometry to perform a comprehensive proteomic analysis of human follicular fluid from healthy ovum donors. Next, using samples from a second set of patients, an isobaric mass tagging strategy for quantitative analysis was used to identify proteins with altered abundances after hCG treatment. RESULTS: A total of 742 follicular fluid proteins were identified in healthy ovum donors, including 413 that have not been previously reported. The proteins belong to diverse functional groups including insulin growth factor and insulin growth factor binding protein families, growth factor and related proteins, receptor signaling, defense/immunity, anti-apoptotic proteins, matrix metalloprotease related proteins, and complement activity. In a quantitative analysis, follicular fluid samples from age-matched women undergoing in vitro fertilization oocyte retrieval were compared and 17 follicular fluid proteins were found at significantly altered levels (p < 0.05) between pre-hCG and post-hCG samples. These proteins belong to a variety of functional processes, including protease inhibition, inflammation, and cell adhesion. CONCLUSIONS: This database of FF proteins significantly extends the known protein components present during the peri-ovulatory period and provides a useful basis for future studies comparing follicular fluid proteomes in various fertility, disease, and environmental exposure conditions. We identified 17 differentially expressed proteins after hCG treatment and together these data showed the feasibility for defining biomarkers that illuminate how the ovarian follicle microenvironment is altered in various infertility-related conditions.
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“There are a large number of renin original substrates AGT in hFF with healthy oocytes [32, 33], and the content ranks 11th–14th [34].”
From Transplantation of human umbilical cord-derived mesenchymal stem cells improves age-related ovarian functional decline via regulating the local renin–angiotensin system on inflammation and oxidative stress (2024) · cites this paper as [34]
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Anderson, N. L., & Anderson, N. G. (2002) · Molecular & Cellular Proteomics · doi:10.1074/mcp.r200007-mcp200 · Cited by 4,414
PANTHER: A Library of Protein Families and Subfamilies Indexed by FunctionPreprint
Thomas, P. D., Campbell, M. J., Kejariwal, A., Mi, H., Karlak, B., Daverman, R., Diemer, K., Muruganujan, A., & Narechania, A. (2003) · Genome Research · doi:10.1101/gr.772403 · Cited by 2,979
Fitzgerald, M., Hayward, I. P., Thomas, A. C., Campbell, J. H., & CAMPBELL, G. R. (1995) · Cited by 2,552
Quantitative mass spectrometric multiple reaction monitoring assays for major plasma proteins
Anderson, L., & Hunter, C. L. (2006) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m500331-mcp200 · Cited by 1,230
Oocyte-secreted factors: regulators of cumulus cell function and oocyte quality
Gilchrist, R. B., Lane, M., & Thompson, J. G. (2008) · Human Reproduction Update · doi:10.1093/humupd/dmm040 · Cited by 1,155
EGF-Like Growth Factors As Mediators of LH Action in the Ovulatory Follicle
Park, J.-Y., Su, Y., Ariga, M., Law, E. C., Jin, S.-L. C., & Conti, M. (2004) · Science · doi:10.1126/science.1092463 · Cited by 1,047
Granulosa Cell Ligand NPPC and Its Receptor NPR2 Maintain Meiotic Arrest in Mouse Oocytes
Zhang, M., Su, Y., Sugiura, K., Xia, G., & Eppig, J. J. (2010) · Science · doi:10.1126/science.1193573 · Cited by 613
Blerkom, J. V., Antczak, M., & Schrader, R. M. (1997) · Human Reproduction · doi:10.1093/humrep/12.5.1047 · Cited by 525
Cyclic GMP from the surrounding somatic cells regulates cyclic AMP and meiosis in the mouse oocyte
Norris, R. P., Ratzan, W. J., Freudzon, M., Mehlmann, L. M., Krall, J., Movsesian, M. A., Wang, H., Ke, H., Nikolaev, V. O., & Jaffe, L. A. (2009) · Development · doi:10.1242/dev.035238 · Cited by 517
Formation of the Ovarian Follicular Antrum and Follicular Fluid1
Rodgers, R. J., & Irving‐Rodgers, H. F. (2010) · Biology of Reproduction · doi:10.1095/biolreprod.109.082941 · Cited by 453
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Mitochondrial Function in Modulating Human Granulosa Cell Steroidogenesis and Female Fertility
Urs, D. B. S., Wu, W.-H., Komrsková, K., Manaskova-Postlerova, P., Lin, Y., Tzeng, C., & Kao, S. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21103592 · Cited by 194
Castillo, J., Jodar, M., & Oliva, R. (2018) · Human Reproduction Update · doi:10.1093/humupd/dmy017 · Cited by 181
Li, H., Huang, X. Y., Chang, X., Yao, J., He, Q., Shen, Z., Ji, Y., & Wang, K. (2019) · Journal of Cellular and Molecular Medicine · doi:10.1111/jcmm.14642 · Cited by 115
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Metadata
- Authors
- Zamah, A. M., Hassis, M. E., Albertolle, M. E., & Williams, K. E.
- Affiliations
- University of Illinois Chicago (US)
- University of California, San Francisco (US)
- Corresponding author
- Zamah, Alberuni M
- Venue
- Clinical Proteomics
- Published
- 2015-03-03
- Volume
- 12
- Issue
- 1
- Pages
- 5-5
- ISSN
- 1542-6416 (ISSN-L)
- 1559-0275
- PMC
- PMC4357057
- OpenAlex
- W2146697938
- Cited by
- 132
- References
- 105
- Citations by year
- 2026: 8 · 2025: 8 · 2024: 11 · 2023: 7 · 2022: 26 · 2021: 6 · 2020: 16 · 2019: 9 · 2018: 16 · 2017: 17 · 2016: 6 · 2015: 2
- FWCI
- 14.66
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Clinical Proteomics (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Figshare (submittedVersion, cc-by)
- Funders
- National Cancer Institute (P30CA082103)
- National Institute of Child Health and Human Development
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (K12HD001262)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Reproductive Biology and Fertility
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Reproductive biology and impacts on aquatic species
- Keywords
- follicular fluid, oocyte maturation, follicle maturation, human chorionic gonadotropin, hCG treatment, differentially expressed proteins, insulin-like growth factor, protease inhibition, inflammation, cell adhesion, infertility
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