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Cited by 19Pesticide and Herbicide Environmental StudiesMicrobial bioremediation and biosurfactantsPharmaceutical and Antibiotic Environmental Impacts

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Insights into the role of hexa-bacterial consortium for bioremediation of soil contaminated with chlorantraniliprole

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Fahmy, Mohamed A., Salem, Samir H., El-Fattah, Hassan I. Abd, Akl, Behairy A., Fayez, Mohamed, Maher, Mohamed, Aioub, Ahmed A. A., Sitohy, Mahmoud·2024·Environmental Sciences Europe
DOI 10.1186/s12302-024-01008-2
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Abstract

This study evaluates the efficacy of discrete bacterial consortia in bioremediating sandy loamy soil deliberately contaminated with 20 mg/kg of chlorantraniliprole (CAP). It monitors alterations in total bacterial populations and CO 2 emissions, tracking residual CAP levels through UV scanning and HPLC analysis. Six active bacterial degraders (four Bacillus strains (B. subtilis subsp. subtilis AZFS3, B. pumilus AZFS5, B. mojavensis AZFS15, and B. paramycoides AZFS18), one Alcaligenes strain (A. aquatilis KZFS11), and one Pseudomonas strain (P. aeruginosa KZFS4)) were used in single or combined preparations and grown on trypticase soy broth for 24 h at 30 °C before preparing the inoculants and adjusting the bacterial cell count to 10 7 CFU/dwt g soil. The bacterial consortia were added to the CAP-contaminated soil and incubated for 20 days at 30 °C. The di-, tetra-, and hexa-bacterial consortia recorded the highest levels of viable bacteria, reaching their peak after 3 to 11 days of incubation. Then, they declined to the minimum levels at the end of the 20 days, which coincided with their complete removal of CAP from the soil. At the end of the incubation period (20 days), the CAP was mainly biodegraded, scoring biodegradation rates of 90.05%, 93.65%, and 98.65% for T3, T4, and T5, respectively. This concurred with the highest average CO 2 production. Based on the results of the HPLC analysis, the hexa-bacterial consortium T5 demonstrated the highest rate of CAP biodegradation (99.33%) after a 20-day incubation period, resulting in the lowest residual level of CAP in the soil (0.67%). Bioinformatic analysis predicted that the CAP biodegradation pathway reached CO 2 and H 2 O. Under optimized conditions, the hexa-bacteria consortium is the most effective CAP biodegraded and is recommended as an eco-friendly treatment for eliminating CAP pollution in the field.

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Research works printed but not linked: 76

  1. Anderson SE, Meade BJ (2014) Potential health effects associated with dermal exposure to occupational chemicals. Environ Health Insights 8:EHI. S15258 · DOI
  2. Mehmood MA et al (2019) Heavy metal contamination in two commercial fish species of a trans-Himalayan freshwater ecosystem. Environ Monit Assess 191(2):104 · DOI
  3. Kadala A, Charreton M, Collet C (2020) Flubendiamide, the first phthalic acid diamide insecticide, impairs neuronal calcium signalling in the honey bee’s antennae. J Insect Physiol 125:104086 · DOI
  4. Khan BA et al (2023) Pesticides: impacts on agriculture productivity, environment, and management strategies. In: Aftab T (ed) Emerging contaminants and plants: interactions, adaptations and remediation technologies. Springer, Cham, pp 109–134 · DOI
  5. Singh AK et al (2018) Distribution of cyanobacteria and their interactions with pesticides in paddy field: a comprehensive review. J Environ Manage 224:361–375 · DOI
  6. Pathak VM et al (2022) Current status of pesticide effects on environment, human health and it’s eco-friendly management as bioremediation: a comprehensive review. Front Microbiol 13:962619 · DOI
  7. Onyeaka H et al (2024) Preventing chemical contaminants in food: challenges and prospects for safe and sustainable food production. Food Control 155:110040 · DOI
  8. Giri BS et al (2021) Progress in bioremediation of pesticide residues in the environment. Environ Eng Res 26(6):200446 · DOI
  9. Kuppan N et al (2024) A comprehensive review of sustainable bioremediation techniques: eco friendly solutions for waste and pollution management. Waste Manag Bull. https://doi.org/10.1016/j.wmb.2024.07.005 · DOI · Paper page
  10. Malik A, Garg VK (2024) Bioremediation for Sustainable Environmental Cleanup. CRC Press, Boca Raton
  11. Richardson EB et al (2020) Diamide resistance: 10 years of lessons from lepidopteran pests. J Pest Sci 93(3):911–928 · DOI
  12. Uesugi R et al (2021) Origin, selection, and spread of diamide insecticide resistance allele in field populations of diamondback moth in east and southeast Asia. Pest Manag Sci 77(1):313–324 · DOI
  13. Lahm GP et al (2007) Rynaxypyr™: a new insecticidal anthranilic diamide that acts as a potent and selective ryanodine receptor activator. Bioorg Med Chem Lett 17(22):6274–6279 · DOI
  14. Sparks DL et al (2020) Methods of soil analysis, part 3: chemical methods, vol 14. Wiley, Hoboken
  15. Li W et al (2019) Residue analysis of tetraniliprole in rice and related environmental samples by HPLC/MS. Microchem J 150:104168 · DOI
  16. Zhang J-M, Chai W-G, Wu Y-L (2012) Residues of chlorantraniliprole in rice field ecosystem. Chemosphere 87(2):132–136 · DOI
  17. Teixeira LA et al (2009) Lethal and sublethal effects of chlorantraniliprole on three species of Rhagoletis fruit flies (Diptera: Tephritidae). Pest Manag Sci 65(2):137–143 · DOI
  18. Dinter A et al (2010) Chlorantraniliprole (Rynaxypyr): a novel DuPont™ insecticide with low toxicity and low risk for honey bees (Apis mellifera) and bumble bees (Bombus terrestris) providing excellent tools for uses in integrated pest management. Julius-Kühn-Archiv 423:84
  19. Lavtižar V et al (2014) Chemical and photochemical degradation of chlorantraniliprole and characterization of its transformation products. Chemosphere 95:408–414 · DOI
  20. Wu M et al (2017) Temporal dynamics of the compositions and activities of soil microbial communities post-application of the insecticide chlorantraniliprole in paddy soils. Ecotoxicol Environ Saf 144:409–415 · DOI
  21. Gao W, Li D, You H (2019) Functional characterization and genomic analysis of the chlorantraniliprole-degrading strain Pseudomonas sp. GW13. Bioengineering 6(4):106 · DOI
  22. Gupta M et al (2016) A study on metabolic prowess of Pseudomonas sp. RPT 52 to degrade imidacloprid, endosulfan and coragen. J Hazard Mater 301:250–258 · DOI
  23. Tewari L, Saini J, Arti (2017) Bioremediation of pesticides by microorganisms: general aspects and recent advances. In: Maheshwari DK (ed) Bioremediation of pollutants. IK International Publishing House Pvt Ltd, New Delhi, pp 24–29
  24. Alegbeleye OO, Opeolu BO, Jackson VA (2017) Polycyclic aromatic hydrocarbons: a critical review of environmental occurrence and bioremediation. Environ Manage 60:758–783 · DOI
  25. Abatenh E et al (2017) The role of microorganisms in bioremediation—a review. Open J Environ Biol 2(1):038–046 · DOI
  26. Cycoń M, Mrozik A, Piotrowska-Seget Z (2017) Bioaugmentation as a strategy for the remediation of pesticide-polluted soil: a review. Chemosphere 172:52–71 · DOI · Paper page
  27. Medfu Tarekegn M, Zewdu Salilih F, Ishetu AI (2020) Microbes used as a tool for bioremediation of heavy metal from the environment. Cogent Food Agric 6(1):1783174 · DOI
  28. Sharma P et al (2021) Critical review on microbial community during in-situ bioremediation of heavy metals from industrial wastewater. Environ Technol Innov 24:101826 · DOI
  29. Hashim HS, Al-Janabi AOH, Al Shammari AOS (2022) Efficiency of Pseudomonas aeruginosa in bioremediation of chlorpyrifos toxicity. Nat Volatiles Essent Oils 9:1024–1033
  30. Lopes P et al (2024) Combination of Pseudomonas aeruginosa and rhamnolipid for bioremediation of soil contaminated with waste lubricant oil. Appl Biochem Microbiol. https://doi.org/10.1134/S0003683823603232 · DOI
  31. Reddy K et al (2024) Microbe-assisted bioremediation of pesticides from contaminated habitats. In: Malik A, Garg VK (eds) Bioremediation for sustainable environmental cleanup. CRC Press, Boca Raton, p 109
  32. Abo-Amer AE (2012) Characterization of a strain of Pseudomonas putida isolated from agricultural soil that degrades cadusafos (an organophosphorus pesticide). World J Microbiol Biotechnol 28(3):805–814 · DOI
  33. Fahmy MA et al (2022) Biodegradation of chlorantraniliprole and flubendiamide by some bacterial strains isolated from different polluted sources. Processes 10(12):2527 · DOI
  34. Page A, Miller R, Keeney D (1982) Methods of soil analysis, part 2. Chem Microbiol Prop 2:643–698
  35. Wu M et al (2018) Rational dose of insecticide chlorantraniliprole displays a transient impact on the microbial metabolic functions and bacterial community in a silty-loam paddy soil. Sci Total Environ 616:236–244 · DOI
  36. Sims JL et al (2019) In situ bioremediation of contaminated unsaturated subsurface soils 1. In: Boulding JR (ed) Epa environmental engineering sourcebook. CRC Press, Boca Raton, pp 165–182 · DOI
  37. Bernard JM (1963) Forest floor moisture capacity of the New Jersey pine barrens. Ecology 44(3):574–576 · DOI
  38. Mukherjee I, Das SK, Kumar A (2016) Degradation of flubendiamide as affected by elevated CO2, temperature, and carbon mineralization rate in soil. Environ Sci Pollut Res 23(19):19931–19939 · DOI
  39. Isermeyer H (1952) Eine einfache Methode zur Bestimmung der Bodenatmung und der Karbonate im Boden. Z Pflanzenernähr Düng Bodenkd 56(1–3):26–38 · DOI
  40. Wollum A (1983) Cultural methods for soil microorganisms. In: Page AL (ed) Methods of soil analysis: part 2 chemical and microbiological properties, vol 9. Wiley, Hoboken, pp 781–802 · DOI
  41. Lehotay SJ, Maštovská K, Yun SJ (2005) Evaluation of two fast and easy methods for pesticide residue analysis in fatty food matrixes. J AOAC Int 88(2):630–638 · DOI
  42. Makino Y et al (2009) Nondestructive analysis of chlorpyrifos on apple skin using UV reflectance. Trans ASABE 52(6):1955–1960 · DOI
  43. Raheem W et al (2022) Pesticide residue measurements in raw milk based on optical spectrum and neural networks. Egypt J Chem 65(2):1–2
  44. Gao J, Ellis LB, Wackett LP (2010) The University of Minnesota biocatalysis/biodegradation database: improving public access. Nucleic Acids Res 38(1):488–491 · DOI
  45. Levío-Raimán M, Bornhardt C, Diez MC (2023) Biodegradation of iprodione and chlorpyrifos using an immobilized bacterial consortium in a packed-bed bioreactor. Microorganisms 11(1):220 · DOI
  46. Varghese EM et al (2024) Biodegradation of chlorpyrifos by an optimized Bacillus consortium isolated from pesticide-contaminated soils of Kerala, India. Int J Pest Manag 70(3):348–356 · DOI
  47. Bhatt P et al (2021) Biotechnological basis of microbial consortia for the removal of pesticides from the environment. Crit Rev Biotechnol 41(3):317–338 · DOI · Paper page
  48. Hussain S et al (2009) Bioremediation and phytoremediation of pesticides: recent advances. Crit Rev Environ Sci Technol 39(10):843–907 · DOI
  49. Uqab B, Mudasir S, Nazir R (2016) Review on bioremediation of pesticides. J Bioremed Biodegr 7(3):343
  50. Feng Y, Minard RD, Bollag JM (1998) Photolytic and microbial degradation of 3, 5, 6-trichloro-2-pyridinol. Environ Toxicol Chem Int J 17(5):814–819 · DOI
  51. Gilani RA et al (2016) Biodegradation of chlorpyrifos by bacterial genus Pseudomonas. J Basic Microbiol 56(2):105–119 · DOI
  52. Anderson J (1984) Herbicide degradation in soil: influence of microbial biomass. Soil Biol Biochem 16(5):483–489 · DOI
  53. Voos G, Groffman P (1997) Relationships between microbial biomass and dissipation of 2, 4-D and dicamba in soil. Biol Fertil Soils 24:106–110 · DOI
  54. Huang Y et al (2018) Microbial degradation of pesticide residues and an emphasis on the degradation of cypermethrin and 3-phenoxy benzoic acid: a review. Molecules 23(9):2313 · DOI
  55. Betancur-Corredor B et al (2015) Evaluation of biostimulation and Tween 80 addition for the bioremediation of long-term DDT-contaminated soil. J Environ Sci 28:101–109 · DOI
  56. Raffa CM, Chiampo F (2021) Bioremediation of agricultural soils polluted with pesticides: a review. Bioengineering 8(7):92 · DOI
  57. Senko O, Maslova O, Efremenko E (2017) Optimization of the use of His6-OPH-based enzymatic biocatalysts for the destruction of chlorpyrifos in soil. Int J Environ Res Public Health 14(12):1438 · DOI
  58. Amani F et al (2018) Biodegradation of chlorpyrifos and diazinon organophosphates by two bacteria isolated from contaminated agricultural soils. Journal of Microbial Biology 7(28):27–39
  59. Liu Z et al (2021) Quorum sensing shaped microbial consortia and enhanced hydrogen recovery from waste activated sludge electro-fermentation on basis of free nitrous acid treatment. Sci Total Environ 766:144348 · DOI
  60. Zhang T, Zhang H (2022) Microbial consortia are needed to degrade soil pollutants. Microorganisms 10(2):261 · DOI
  61. Chaudhary P et al (2023) Application of synthetic consortia for improvement of soil fertility, pollution remediation, and agricultural productivity: a review. Agronomy 13(3):643 · DOI
  62. Lü H et al (2024) Microbial consortium degrading of organic pollutants: Source, degradation efficiency, pathway, mechanism and application. J Clean Prod 451:141913 · DOI
  63. Chen R et al (2024) Recent advances and optimization strategies for the microbial degradation of PCBs: from monocultures to microbial consortia. Crit Rev Environ Sci Technol 54(14):1023–1049 · DOI
  64. Migita C, Chiba Y, Kenzo T (2023) Direct measurement of the three-dimensional distribution of leaf area density and light conditions in a mature oak stand by the cube method. J For Res 34(6):1817–1827 · DOI
  65. Sabaté J, Vinas M, Solanas A (2004) Laboratory-scale bioremediation experiments on hydrocarbon-contaminated soils. Int Biodeterior Biodegrad 54(1):19–25 · DOI
  66. Ataikiru T, Okpokwasili G, Okerentugba P (2019) Impact of pesticides on microbial diversity and enzymes in soil. South Asian J Res Microbiol 4(2):1–16 · DOI
  67. Zhang C et al (2021) Enhancement of dicarboximide fungicide degradation by two bacterial cocultures of Providencia stuartii JD and Brevundimonas naejangsanensis J3. J Hazard Mater 403:123888 · DOI
  68. Vieira GAL et al (2021) Marine associated microbial consortium applied to RBBR textile dye detoxification and decolorization: combined approach and metatranscriptomic analysis. Chemosphere 267:129190 · DOI
  69. Xu B et al (2020) Characterization of acetamiprid biodegradation by the microbial consortium ACE-3 enriched from contaminated soil. Front Microbiol 11:1429 · DOI
  70. Zhang Y et al (2022) Plant and microorganism combined degradation of bensulfuron herbicide in eight different agricultural soils. Agronomy 12(12):2989 · DOI
  71. Fuentes MS et al (2017) Removal of a mixture of pesticides by a Streptomyces consortium: influence of different soil systems. Chemosphere 173:359–367 · DOI
  72. Liu H, Dong X, Meng Y, Gao T, Mao L, Gao R (2023) A novel model to evaluate spatial structure in thinned conifer-broadleaved mixed natural forests. J For Res 34(6):1881–1898 · DOI
  73. Góngora-Echeverría VR et al (2020) Pesticide bioremediation in liquid media using a microbial consortium and bacteria-pure strains isolated from a biomixture used in agricultural areas. Ecotoxicol Environ Saf 200:110734 · DOI
  74. Chang E, Liu X, Chen J, Sun J, Yang S, Liu J (2023) Comparative transcriptomes reveal the disjunction adaptive strategy of Thuja species in East Asia and North America. J For Res 34(6):1963–1974 · DOI
  75. Agarry S et al (2013) Biodegradation of dichlorovos (organophosphate pesticide) in soil by bacterial isolates. Biodegradation 3(8):11–16
  76. Das C et al (2023) Ecological effects of emerging contaminants: Ecotoxicity and relative environmental risk. In: Kumar M, Mohapatra S, Weber K (eds) Emerging Aquatic Contaminants. Elsevier, Amsterdam, pp 375–404 · DOI

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Metadata

Authors
Fahmy, M. A., Salem, S. H., El-Fattah, H. I. A., Akl, B. A., Fayez, M., Maher, M., Aioub, A. A. A., & Sitohy, M.
Affiliations
Zagazig University (EG)
Corresponding author
  • Aioub, Ahmed A. A.
  • Sitohy, Mahmoud
ORCID
Venue
Environmental Sciences Europe
Publisher
Springer Science and Business Media LLC
Published
2024-11-18
Volume
36
Issue
1
ISSN
2190-4715 (ISSN-L)
DOI
10.1186/s12302-024-01008-2
OpenAlex
W4404468499
Cited by
19
References
0
Citations by year
2026: 8 · 2025: 11
FWCI
1.79
Type
journal article
Language
en
Update notices
correction (2025-06-14)
Open access
Licence
Locations
  • Environmental Sciences Europe (publishedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
Zagazig University
Topics
  • Physical Sciences › Environmental Science › Pollution › Pesticide and Herbicide Environmental Studies
  • Physical Sciences › Environmental Science › Pollution › Microbial bioremediation and biosurfactants
  • Physical Sciences › Environmental Science › Pollution › Pharmaceutical and Antibiotic Environmental Impacts
Keywords
chlorantraniliprole, bioremediation, bacterial consortium, soil contamination, Bacillus, Pseudomonas aeruginosa, Alcaligenes aquatilis, insecticide biodegradation, CO2 production, biodegradation pathways, sandy loam soil

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