CoChat
Sign Up
Cited by 16Fungal Infections and StudiesParasitic Infections and DiagnosticsAntifungal resistance and susceptibility

The DOI matches its Crossref record; 39 of the 39 references it cites were found in a registry or an index.

Application and evaluation of nucleic acid sequence-based amplification, PCR and cryptococcal antigen test for diagnosis of cryptococcosis

Open access
Wang, Yanping, Yang, Mi, Xia, Yun, Yan, Jia, Zou, Jiaqi, Zhang, Dawei·2021·BMC Infectious Diseases
DOI 10.1186/s12879-021-06678-4PMC PMC8482667
Open this paper in CoChat — free accountOpen PDF

Abstract

BACKGROUND: Cryptococcosis is a major opportunistic invasive mycosis in immunocompromised patients, but it is also increasingly seen in immunocompetent patients. In the early stages of cryptococcosis, limitations of the detection method may hinder the diagnosis. A molecular diagnostic technique based on nucleic acid sequence-based amplification (NASBA) method was developed to fulfil the need for efficient diagnosis of cryptococcosis. METHODS: We compared the diagnostic performance of NASBA, PCR and cryptococcal antigen (CrAg) test (colloidal gold method) in clinical samples from 25 cryptococcosis patients (including 8 cryptococcal meningoencephalitis and 17 pulmonary cryptococcosis) who were categorized as proven cases (n = 10) and probable cases (n = 15) according to the revised EORTC/MSG definitions. 10 patients with non-Cryptococcus infection and 30 healthy individuals were categorized as control group. RESULTS: The lowest detection limit of NASBA was 10 CFU/mL, and RNA of non-target bacteria or fungi was not amplified. The sensitivity of NASBA, PCR and colloidal gold method was 92.00% (95% CI 72.50-98.60%), 64.00% (95% CI 42.62-81.29%), 100.00% (95% CI 83.42-100.00%), and the specificity was 95.00% (95% CI 81.79-99.13%), 80.00% (95% CI 63.86-90.39%) and 82.50% (95% CI 66.64-92.11%) respectively. The highest specificity (97.50%), accuracy (95.38%) and k value (0.90) were achieved when both NASBA and colloidal gold results were positive. CONCLUSIONS: NASBA is a new alternative detection method for cryptococcosis which is both accurate and rapid without expensive equipment and specialised personnel. It may be used as a tool for confirming current infection as well as monitoring the effectiveness of antifungal treatment. The use of NASBA to detect Cryptococcus RNA in blood samples is of great significance for the diagnosis of pulmonary cryptococcosis. The combination of NASBA and colloidal gold can improve the diagnostic accuracy of cryptococcosis.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1186/s12879-021-06678-4

Cites

39 of 39 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • LAMP and NASBA have been studied in various CNS infections and might especially be useful in laboratories with limited resources.

    “LAMP and NASBA have been studied in various CNS infections and might especially be useful in laboratories with limited resources (26–31).”

    From Molecular diagnostics in cerebrospinal fluid for the diagnosis of central nervous system infections (2024) · cites this paper as [31]

    The Conclusions (page unavailable) say NASBA is suitable for less developed laboratories because it needs no expensive equipment or specialised personnel. The paper studies NASBA for cryptococcal infection, including meningoencephalitis, but does not address LAMP or establish use across various CNS infections. It also does not recommend NASBA testing of CSF for cryptococcal meningoencephalitis because antigen detection is sufficiently sensitive. (LLM, conf=0.88)

    Experimental — accuracy not yet measured

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Related

Metadata

Authors
Wang, Y., Yang, M., Xia, Y., Yan, J., Zou, J., & Zhang, D.
Affiliations
  • The Affiliated Yongchuan Hospital of Chongqing Medical University (CN)
  • University of Electronic Science and Technology of China (CN)
Corresponding author
Xia, Yun
ORCID
Venue
BMC Infectious Diseases
Publisher
Springer Science and Business Media LLC
Published
2021-09-29
Volume
21
Issue
1
Pages
1020-1020
ISSN
1471-2334 (ISSN-L)
DOI
10.1186/s12879-021-06678-4
PMC
PMC8482667
OpenAlex
W3202521942
Cited by
16
References
39
Citations by year
2026: 1 · 2025: 3 · 2024: 9 · 2023: 2 · 2022: 1
FWCI
2.16
Type
journal article
Language
en
Open access
Licence
Locations
  • BMC Infectious Diseases (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, other-oa)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • the Project of National Clinical Key-discipline Construction (2010 (305))
  • the Fundamental and Front Research Funds of Chongqing (20160129)
  • the Project of Precision Medical Data Integration Technology Research and Big Data Analysis Construction (2017YFC0909902)
Topics
  • Health Sciences › Medicine › Infectious Diseases › Fungal Infections and Studies
  • Life Sciences › Immunology and Microbiology › Parasitology › Parasitic Infections and Diagnostics
  • Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
Keywords
cryptococcosis, NASBA, cryptococcal antigen test, cryptococcal meningoencephalitis, pulmonary cryptococcosis, Cryptococcus neoformans, molecular diagnosis, sensitivity and specificity, immunocompromised patients, opportunistic fungal infection, PCR
MeSH terms
Antigens, Fungal, Cryptococcosis, Cryptococcus, Humans, Sensitivity and Specificity, Polymerase Chain Reaction, Self-Sustained Sequence Replication

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review