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Cited by 41Advanced Glycation End Products researchBone health and osteoporosis researchBone Metabolism and Diseases

Non-invasive skin autofluorescence, blood and urine assays of the advanced glycation end product (AGE) pentosidine as an indirect indicator of AGE content in human bone

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Kida, Yoshikuni, Saito, Mitsuru, Shinohara, Akira, Soshi, Shigeru, Marumo, Keishi·2019·BMC Musculoskeletal Disorders
DOI 10.1186/s12891-019-3011-4PMC PMC6933723
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Abstract

BACKGROUND: Bone mineral density (BMD) measurements are widely used to assess fracture risk. However, the finding that some fracture patients had high BMD together with the low contribution of drugs to osteoporosis suggests that bone strength factors other than BMD contribute to bone quality. We evaluated the amount of advanced glycation end products (AGEs) by non-invasive assays of serum and urine as well as by skin autofluorescence to measure the levels of a representative AGE, pentosidine, to investigate whether pentosidine can serve as an indirect indicator of AGEs formation in bone collagen. METHODS: A total of 100 spinal surgery patients without fragility fracture (54 males and 46 females) treated at our hospital were enrolled. The amount of pentosidine in blood, urine, skin and bone (lumbar lamina) samples from these patients was measured. AGE accumulation was assessed by measuring skin autofluorescence. We examined the correlation between pentosidine content in tissues and body fluid, as well as skin AGEs with age, height, body weight, BMI, and estimated glomerular filtration rate (eGFR). RESULTS: A significant age-related increase in pentosidine levels in tissues was observed, while there was a significant negative correlation between tissue pentosidine and eGFR. The amount of skin pentosidine was significantly and positively correlated with pentosidine content of the bone in those under 50 years of age. Urine pentosidine also correlated positively with bone pentosidine and skin pentosidine, but only in females. The total amount of AGEs in skin did not correlate with bone pentosidine. CONCLUSION: In this study, the strong correlation between the pentosidine content in each sample and eGFR may indicate that renal dysfunction with advancing age increases oxidative stress and induces AGEs formation in collagen-containing tissues. The correlation of skin pentosidine concentration and eGFR, with AGEs formation in bone collagen suggests that pentosidine would be a useful indirect index of decreased bone quality. Skin AGEs estimated by autofluorescence in clinical situations may not be suitable as an indirect assessment of bone quality. Because urine pentosidine correlated positively with bone pentosidine and skin pentosidine in females, urine pentosidine may be a candidate for an indirect assessment of bone quality.

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  • Kida et al. found strong correlations between serum, urine, bone, and skin pentosidine levels with age in spinal surgery patients.

    “Kida and colleagues performed their analysis in different specimens of spinal surgery patients and found strong correlations between serum, urine, bone, and skin pentosidine levels with age [170].”

    From Protein oxidation - Formation mechanisms, detection and relevance as biomarkers in human diseases (2021) · cites this paper as [170]

    The Methods describe 100 spinal surgery patients, and Table 2 in the Results reports significant positive correlations with age for serum, urine, bone, and skin pentosidine in the full cohort. However, the correlations range from r = 0.359 to 0.525, so describing all four as strong overstates the results; some age-group correlations were not significant. No page number is available in our copy. (LLM, conf=0.90)

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  • Studies from 2019 suggest an age-related increase in skin AF, pentosidine levels, and total amount of AGEs.

    “studies from 2019 suggest an age-related increase in skin AF, pentosidine levels and total amount of AGEs [141,170,171]”

    From Protein oxidation - Formation mechanisms, detection and relevance as biomarkers in human diseases (2021) · cites this paper as [170]

  • Pentosidine levels increase with age in serum, urine, bone and skin.

    “| >Aging (and Diabetes) | Skin, urine, serum, bone | Patients with spine disease | Skin AF,ELISA,HPLC | Age-related increase in serum, urine, bone and skin pentosidine levels | [170] |”

    From Protein oxidation - Formation mechanisms, detection and relevance as biomarkers in human diseases (2021) · cites this paper as [170]

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Cited by

Sorted by citations

Authors
Kida, Y., Saito, M., Shinohara, A., Soshi, S., & Marumo, K.
Affiliations
Jikei University School of Medicine (JP)
Corresponding author
Saito, Mitsuru
ORCID
Venue
BMC Musculoskeletal Disorders
Publisher
Springer Science and Business Media LLC
Published
2019-12
Volume
20
Issue
1
Pages
627-627
ISSN
1471-2474 (ISSN-L)
DOI
10.1186/s12891-019-3011-4
PMC
PMC6933723
OpenAlex
W2997680497
Cited by
41
References
1
Citations by year
2026: 4 · 2025: 5 · 2024: 8 · 2023: 6 · 2022: 3 · 2021: 10 · 2020: 5
FWCI
3.11
Type
journal article
Language
en
Open access
Licence
Locations
  • BMC Musculoskeletal Disorders (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Jikei University School of Medicine
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Advanced Glycation End Products research
  • Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
Keywords
advanced glycation end products, pentosidine, skin autofluorescence, bone quality, bone collagen, non-invasive biomarkers, estimated glomerular filtration rate, renal dysfunction, oxidative stress, osteoporosis, fragility fractures, spinal surgery patients
MeSH terms
Adult, Aged, Arginine, Bone and Bones, Collagen, Female, Humans, Lysine, Male, Middle Aged, Osteoporosis, Sex Factors, Skin, Bone Density, Glycation End Products, Advanced, Oxidative Stress, Osteoporotic Fractures, Optical Imaging

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