HyperART: non-invasive quantification of leaf traits using hyperspectral absorption-reflectance-transmittance imaging
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BACKGROUND: Combined assessment of leaf reflectance and transmittance is currently limited to spot (point) measurements. This study introduces a tailor-made hyperspectral absorption-reflectance-transmittance imaging (HyperART) system, yielding a non-invasive determination of both reflectance and transmittance of the whole leaf. We addressed its applicability for analysing plant traits, i.e. assessing Cercospora beticola disease severity or leaf chlorophyll content. To test the accuracy of the obtained data, these were compared with reflectance and transmittance measurements of selected leaves acquired by the point spectroradiometer ASD FieldSpec, equipped with the FluoWat device. RESULTS: The working principle of the HyperART system relies on the upward redirection of transmitted and reflected light (range of 400 to 2500 nm) of a plant sample towards two line scanners. By using both the reflectance and transmittance image, an image of leaf absorption can be calculated. The comparison with the dynamically high-resolution ASD FieldSpec data showed good correlation, underlying the accuracy of the HyperART system. Our experiments showed that variation in both leaf chlorophyll content of four different crop species, due to different fertilization regimes during growth, and fungal symptoms on sugar beet leaves could be accurately estimated and monitored. The use of leaf reflectance and transmittance, as well as their sum (by which the non-absorbed radiation is calculated) obtained by the HyperART system gave considerably improved results in classification of Cercospora leaf spot disease and determination of chlorophyll content. CONCLUSIONS: The HyperART system offers the possibility for non-invasive and accurate mapping of leaf transmittance and absorption, significantly expanding the applicability of reflectance, based on mapping spectroscopy, in plant sciences. Therefore, the HyperART system may be readily employed for non-invasive determination of the spatio-temporal dynamics of various plant properties.
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Chlorophyll extraction took place in a dark room because chlorophyll is light sensitive.
“Since chlorophyll is light sensitive, extraction took place in a dark room (Bergsträsser et al., 2015).”
From Growth, photosynthetic function, and stomatal characteristics of Persian walnut explants in vitro under different light spectra (2023) · cites this paper as Bergsträsser et al., 2015
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- Authors
- Bergsträsser, S., Fanourakis, D., Schmittgen, S., Cendrero-Mateo, M., Jansen, M., Scharr, H., & Rascher, U.
- Affiliations
- Forschungszentrum Jülich (DE)
- Corresponding author
- Bergsträsser, Sergej
- Venue
- Plant Methods
- Published
- 2015
- Volume
- 11
- Issue
- 1
- Pages
- 1
- ISSN
- 1746-4811 (ISSN-L)
- PMC
- PMC4302522
- OpenAlex
- W2160038364
- Cited by
- 209
- References
- 57
- Citations by year
- 2026: 7 · 2025: 12 · 2024: 19 · 2023: 16 · 2022: 14 · 2021: 30 · 2020: 38 · 2019: 14 · 2018: 11 · 2017: 22 · 2016: 16 · 2015: 10
- FWCI
- 17.83
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Plant Methods (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- JuSER (Forschungszentrum Jülich) (submittedVersion, other-oa)
- Funders
- European Commission (284443)
- Bundesministerium für Bildung und Forschung (315309)
- European Regional Development Fund
- Topics
- Physical Sciences › Environmental Science › Ecology › Remote Sensing in Agriculture
- Physical Sciences › Chemistry › Analytical Chemistry › Spectroscopy and Chemometric Analyses
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Smart Agriculture and AI
- Keywords
- hyperspectral imaging, leaf reflectance, leaf transmittance, leaf absorption, Cercospora beticola, chlorophyll content, plant disease severity, fertilization regimes, line scanner
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