The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.
Ontogeny of CpG island methylation and specificity of DNMT3 methyltransferases during embryonic development in the mouse
Open accessAbstract
Background: In the mouse, the patterns of DNA methylation are established during early embryonic development in the epiblast. We quantified the targets and kinetics of DNA methylation acquisition in epiblast cells, and determined the contribution of the de novo methyltransferases DNMT3A and DNMT3B to this process. Results: We generated single-base maps of DNA methylation from the blastocyst to post-implantation stages and in embryos lacking DNMT3A or DNMT3B activity. DNA methylation is established within two days of implantation between embryonic days 4.5 and 6.5. The kinetics of de novo methylation are uniform throughout the genome, suggesting a random mechanism of deposition. In contrast, many CpG islands acquire methylation slowly in late epiblast cells. Five percent of CpG islands gain methylation and are found in the promoters of germline genes and in exons of important developmental genes. The onset of global methylation correlates with the upregulation of Dnmt3a/b genes in the early epiblast. DNMT3A and DNMT3B act redundantly to methylate the bulk genome and repetitive elements, whereas DNMT3B has a prominent role in the methylation of CpG islands on autosomes and the X chromosome. Reduced CpG island methylation in Dnmt3b-deficient embryos correlates with gene reactivation in promoters but reduced transcript abundance in gene bodies. Finally, DNMT3B establishes secondary methylation marks at imprinted loci, which distinguishes bona fide germline from somatic methylation imprints. Conclusions: We reveal that the DNMT3 de novo methyltransferases play both redundant and specific functions in the establishment of DNA methylation in the mouse embryo.
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Conserved role of intragenic DNA methylation in regulating alternative promoters
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DNA-binding factors shape the mouse methylome at distal regulatory regions
Stadler, M. B., Murr, R., Burger, L., Ivanek, R., Lienert, F., Schöler, A., Nimwegen, E. V., Wirbelauer, C., Oakeley, E. J., Gaidatzis, D., et al. (2011) · Nature · doi:10.1038/nature10716 · Cited by 1,383
De novo DNA cytosine methyltransferase activities in mouse embryonic stem cells
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Orphan CpG Islands Identify Numerous Conserved Promoters in the Mammalian Genome
Illingworth, R. S., Gruenewald-Schneider, U., Webb, S., Kerr, A. R. W., James, K. D., Turner, D. J., Smith, C., Harrison, D. J., Andrews, R., & Bird, A. P. (2010) · PLoS Genetics · doi:10.1371/journal.pgen.1001134 · Cited by 550
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“This remarkable specificity, together with the fact that germline genes are durably reactivated in methylation-deficient embryos and cultured cells as shown by us and others [11,46-48], indicates that the silencing of the germline program in soma is a major evolutionary function of DNA methylation in mammals.”
BackgroundGlobal survey of escape from X inactivation by RNA-sequencing in mouse
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Loss of Tet Enzymes Compromises Proper Differentiation of Embryonic Stem Cells
Dawlaty, M. M., Breiling, A., Le, T., Barrasa, M. I., Raddatz, G., Gao, Q., Powell, B. E., Cheng, A. W., Faull, K. F., Lyko, F., et al. (2014) · Developmental Cell · doi:10.1016/j.devcel.2014.03.003 · Cited by 338
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The DNA methyltransferase DNMT3C protects male germ cells from transposon activity
Barau, J., Teissandier, A., Zamudio, N., Roy, S., Nalesso, V., Hérault, Y., Guillou, F., & Bourc’his, D. (2016) · Science · doi:10.1126/science.aah5143 · Cited by 389
TET-mediated DNA demethylation controls gastrulation by regulating Lefty-Nodal signalling
Dai, H.-Q., Wang, B.-A., Yang, L., Chen, J.-J., Zhu, G.-C., Sun, M.-L., Ge, H., Wang, R., Chapman, D. L., Tang, F., et al. (2016) · Nature · doi:10.1038/nature20095 · Cited by 206
Epigenetic restriction of extraembryonic lineages mirrors the somatic transition to cancer
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Metadata
- Authors
- Auclair, G., Guibert, S., Bender, A., & Weber, M.
- Venue
- Genome Biology
- Published
- 2014-12-03
- Volume
- 15
- Issue
- 12
- Pages
- 545-545
- ISSN
- 1465-6906 (ISSN-L)
- 1474-760X
- OpenAlex
- W2124933556
- Cited by
- 189
- References
- 8
- FWCI
- 4.02
- Type
- journal article
- Language
- en
- Open access
- gold
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- Auclair, G., Guibert, S., Bender, A., & Weber, M. (2014). Ontogeny of CpG island methylation and specificity of DNMT3 methyltransferases during embryonic development in the mouse. Genome Biology. https://doi.org/10.1186/s13059-014-0545-5
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