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Cited by 266Genomics and Chromatin DynamicsEpigenetics and DNA MethylationChromatin Remodeling and Cancer

Histone H3K27 acetylation is dispensable for enhancer activity in mouse embryonic stem cells

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Zhang, Tiantian, Zhang, Zhuqiang, Dong, Qiang, Xiong, Jun, Zhu, Bing·2020·Genome Biology
DOI 10.1186/s13059-020-01957-wPMC PMC7035716
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Abstract

H3K27ac is well recognized as a marker for active enhancers and a great indicator of enhancer activity. However, its functional impact on transcription has not been characterized. By substituting lysine 27 in histone variant H3.3 with arginine in mouse embryonic stem cells, we diminish the vast majority of H3K27ac at enhancers. However, the transcriptome is largely undisturbed in these mutant cells, likely because the other enhancer features remain largely unchanged, including chromatin accessibility, H3K4me1, and histone acetylation at other lysine residues. Our results clearly reveal that H3K27ac alone is not capable of functionally determining enhancer activity.

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  • The H3K27ac mark is not required for enhancer function, at least in mouse embryonic stem cells.

    “For example, enhancers devoid of the classic H3K27ac mark have recently been described [163]; consistent with this, it was shown that this mark is not required for enhancer function, at least in mouse ESCs [164].”

    From Transcriptional enhancers and their communication with gene promoters (2021) · cites this paper as [164]

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Cited by

Sorted by citations

Authors
Zhang, T., Zhang, Z., Dong, Q., Xiong, J., & Zhu, B.
Affiliations
Chinese Academy of Sciences (CN)
Corresponding author
  • Xiong, Jun
  • Zhu, Bing
ORCID
Venue
Genome Biology
Publisher
Springer Science and Business Media LLC
Published
2020-02-21
Volume
21
Issue
1
Pages
45-45
ISSN
  • 1465-6906 (ISSN-L)
  • 1474-760X
DOI
10.1186/s13059-020-01957-w
PMC
PMC7035716
OpenAlex
W3008303413
Cited by
266
References
4
Citations by year
2026: 29 · 2025: 43 · 2024: 44 · 2023: 42 · 2022: 48 · 2021: 39 · 2020: 21
FWCI
12.51
Type
journal article
Language
en
Open access
Licence
Locations
  • Genome biology (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Ministry of Science and Technology of the People's Republic of China (2016YFA0100400, 2018YFE0203300)
  • National Natural Science Foundation of China (31530037, 31425013, 31521002)
  • Youth Innovation Promotion Association of the Chinese Academy of Sciences (2017133, 2020097)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Chromatin Remodeling and Cancer
Keywords
H3K27 acetylation, enhancer activity, histone H3.3, mouse embryonic stem cells, chromatin accessibility, H3K4me1, histone acetylation, enhancer function, transcriptome
MeSH terms
Mouse Embryonic Stem Cells, Acetylation, Animals, Cells, Cultured, Enhancer Elements, Genetic, Histones, Lysine, Mutation, Histone Code, Mice, Transcriptome

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