Widespread impact of transposable elements on the evolution of post-transcriptional regulation in the cotton genus Gossypium
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Abstract
BACKGROUND: Transposable element (TE) expansion has long been known to mediate genome evolution and phenotypic diversity in organisms, but its impact on the evolution of post-transcriptional regulation following species divergence remains unclear. RESULTS: To address this issue, we perform long-read direct RNA sequencing, polysome profiling sequencing, and small RNA sequencing in the cotton genus Gossypium, the species of which range more than three folds in genome size. We find that TE expansion contributes to the turnover of transcription splicing sites and regulatory sequences, leading to changes in alternative splicing patterns and the expression levels of orthologous genes. We also find that TE-derived upstream open reading frames and microRNAs serve as regulatory elements mediating differences in the translation levels of orthologous genes. We further identify genes that exhibit lineage-specific divergence at the transcriptional, splicing, and translational levels, and showcase the high flexibility of gene expression regulation in the evolutionary process. CONCLUSIONS: Our work highlights the significant role of TE in driving post-transcriptional regulation divergence in the cotton genus. It offers insights for deciphering the evolutionary mechanisms of cotton species and the formation of biological diversity.
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- Authors
- Tian, X., Wang, R., Liu, Z., Lu, S., Chen, X., Zhang, Z., Liu, F., Li, H., Zhang, X., & Wang, M.
- Affiliations
- Huazhong Agricultural University (CN)
- Chinese Academy of Agricultural Sciences (CN)
- Shihezi University (CN)
- Corresponding author
- Wang, Maojun
- ORCID
- Venue
- Genome Biology
- Published
- 2025-03-17
- Volume
- 26
- Issue
- 1
- Pages
- 60-60
- ISSN
- 1465-6906 (ISSN-L)
- 1474-760X
- PMC
- PMC11912738
- OpenAlex
- W4408529910
- Cited by
- 16
- References
- 11
- Citations by year
- 2026: 13 · 2025: 3
- FWCI
- 8.65
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Genome biology (publishedVersion, cc-by-nc-nd)
- Research Square (acceptedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Natural Science Foundation of China (32170645)
- National Key Research and Development Program of China (2021YFF1000900)
- the Foundation of Hubei Hongshan Laboratory (2021hszd014)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Chromosomal and Genetic Variations
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Research in Cotton Cultivation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Keywords
- transposable elements, post-transcriptional regulation, Gossypium, alternative splicing, upstream open reading frames, polysome profiling, genome expansion
- MeSH terms
- Gossypium, DNA Transposable Elements, RNA Processing, Post-Transcriptional, Alternative Splicing, Gene Expression Regulation, Plant, Genome, Plant, Evolution, Molecular, MicroRNAs
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