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Cited by 410Mesenchymal stem cell researchPeriodontal Regeneration and TreatmentsPluripotent Stem Cells Research

Comparative analysis of human mesenchymal stem cells from bone marrow and adipose tissue under xeno-free conditions for cell therapy

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Li, Chun-yu, Wu, Xiao-yun, Tong, Jia-bei, Yang, Xin-xin, Zhao, Jing-li, Zheng, Quan-fu, Zhao, Guo-bin, Ma, Zhi-jie·2015·Stem Cell Research & Therapy
DOI 10.1186/s13287-015-0066-5PMC PMC4453294
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Abstract

INTRODUCTION: Mesenchymal stem cells (MSCs) are promising candidates for cell-based therapies. Human platelet lysate represents an efficient alternative to fetal bovine serum for clinical-scale expansion of MSCs. Different media used in culture processes should maintain the biological characteristics of MSCs during multiple passages. However, bone marrow-derived MSCs and adipose tissue-derived MSCs have not yet been directly compared with each other under human platelet lysate conditions. This study aims to conduct a direct head-to-head comparison of the biological characteristics of the two types of MSCs under human platelet lysate-supplemented culture conditions for their ability to be used in regenerative medicine applications. METHODS: The bone marrow- and adipose tissue-derived MSCs were cultured under human platelet lysate conditions and their biological characteristics evaluated for cell therapy (morphology, immunophenotype, colony-forming unit-fibroblast efficiency, proliferation capacity, potential for mesodermal differentiation, secreted proteins, and immunomodulatory effects). RESULTS: Under human platelet lysate-supplemented culture conditions, bone marrow- and adipose tissue-derived MSCs exhibited similar fibroblast-like morphology and expression patterns of surface markers. Adipose tissue-derived MSCs had greater proliferative potential than bone marrow-derived MSCs, while no significantly difference in colony efficiency were observed between the two types of cells. However, bone marrow-derived MSCs possessed higher capacity toward osteogenic and chondrogenic differentiation compared with adipose tissue-derived MSCs, while similar adipogenic differentiation potential wase observed between the two types of cells. There were some differences between bone marrow- and adipose tissue-derived MSCs for several secreted proteins, such as cytokine (interferon-γ), growth factors (basic fibroblast growth factor, hepatocyte growth factor, and insulin-like growth factor-1), and chemokine (stem cell-derived factor-1). Adipose tissue-derived MSCs had more potent immunomodulatory effects than bone marrow-derived MSCs. CONCLUSIONS: Adipose tissue-derived MSCs have biological advantages in the proliferative capacity, secreted proteins (basic fibroblast growth factor, interferon-γ, and insulin-like growth factor-1), and immunomodulatory effects, but bone marrow-derived MSCs have advantages in osteogenic and chondrogenic differentiation potential and secreted proteins (stem cell-derived factor-1 and hepatocyte growth factor); these biological advantages should be considered systematically when choosing the MSC source for specific clinical application.

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Cited by

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Authors
Li, C.-Y., Wu, X.-Y., Tong, J.-B., Yang, X.-X., Zhao, J.-L., Zheng, Q.-F., Zhao, G.-B., & Ma, Z.-J.
Affiliations
  • Shandong First Medical University (CN)
  • Changchun University of Chinese Medicine (CN)
  • Jilin Vocational College of Industry and Technology (CN)
  • 302 Military Hospital of China (CN)
  • Hebei North University (CN)
  • Capital Medical University (CN)
Corresponding author
Ma, Zhi-jie
ORCID
Tong, Jia-bei
Venue
Stem Cell Research & Therapy
Publisher
Springer Science and Business Media LLC
Published
2015-04-13
Volume
6
Issue
1
Pages
55-55
ISSN
1757-6512 (ISSN-L)
DOI
10.1186/s13287-015-0066-5
PMC
PMC4453294
OpenAlex
W1976604464
Cited by
410
References
3
Citations by year
2026: 21 · 2025: 13 · 2024: 43 · 2023: 39 · 2022: 42 · 2021: 27 · 2020: 60 · 2019: 40 · 2018: 39 · 2017: 45 · 2016: 33 · 2015: 7
FWCI
23.14
Type
journal article
Language
en
Open access
Licence
Locations
  • Stem Cell Research & Therapy (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Natural Science Foundation of China
Topics
  • Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
  • Health Sciences › Medicine › Urology › Periodontal Regeneration and Treatments
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
Keywords
mesenchymal stem cells, bone marrow-derived mesenchymal stem cells, adipose-derived stem cells, human platelet lysate, xeno-free culture, cell therapy, osteogenic differentiation, chondrogenic differentiation, adipogenesis, immunomodulation, secreted proteins, interferon-gamma, basic fibroblast growth factor, hepatocyte growth factor, insulin-like growth factor I, cell proliferation, immunophenotype
MeSH terms
Adipose Tissue, Blood Platelets, Bone Marrow Cells, Cell Differentiation, Cells, Cultured, Enzyme-Linked Immunosorbent Assay, Humans, Insulin-Like Growth Factor I, Mesoderm, Antigens, CD, Immunophenotyping, Cytokines, Fibroblast Growth Factor 2, Hepatocyte Growth Factor, Cell Proliferation, Chemokine CXCL12, Mesenchymal Stem Cells, Cell- and Tissue-Based Therapy

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