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Near-infrared light induces neurogenesis and modulates anxiety-like behavior
Open accessPublished version of a preprint
Abstract
BACKGROUND: The hippocampus is associated with mood disorders, and the activation of quiescent neurogenesis has been linked to anxiolytic effects. Near-infrared (NIR) light has shown potential to improve learning and memory in human and animal models. Despite the vast amount of information regarding the effect of visible light, there is a significant gap in our understanding regarding the response of neural stem cells (NSCs) to NIR stimulation, particularly in anxiety-like behavior. The present study aimed to develop a new optical manipulation approach to stimulate hippocampal neurogenesis and understand the mechanisms underlying its anxiolytic effects. METHODS: We used 940 nm NIR (40 Hz) light exposure to stimulate hippocampal stem cells in C57BL/6 mice. The enhanced proliferation and astrocyte differentiation of NIR-treated NSCs were assessed using 5-ethynyl-2'-deoxyuridine (EdU) incorporation and immunofluorescence assays. Additionally, we evaluated calcium activity of NIR light-treated astrocytes using GCaMP6f recording through fluorescence fiber photometry. The effects of NIR illumination of the hippocampus on anxiety-like behaviors were evaluated using elevated plus maze and open-field test. RESULTS: NIR light effectively promoted NSC proliferation and astrocyte differentiation via the OPN4 photoreceptor. Furthermore, NIR stimulation significantly enhanced neurogenesis and calcium-dependent astrocytic activity. Moreover, activating hippocampal astrocytes with 40-Hz NIR light substantially improved anxiety-like behaviors in mice. CONCLUSIONS: We found that flickering NIR (940 nm/40Hz) light illumination improved neurogenesis in the hippocampus with anxiolytic effects. This innovative approach holds promise as a novel preventive treatment for depression.
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Metadata
- Authors
- Qi, X., Xu, Z., Liu, X., Ren, Y., Jin, Y., Sun, W., Li, J., Liu, D., Liu, S., Liu, Q., & Li, X.
- Affiliations
- Shandong University (CN)
- Shandong Sport University (CN)
- Corresponding author
- Liu, Duo
- Liu, Shuwei
- Liu, Qiji
- Li, Xi
- ORCID
- Venue
- Stem Cell Research & Therapy
- Published
- 2024-12-20
- Volume
- 15
- Issue
- 1
- Pages
- 494-494
- ISSN
- 1757-6512 (ISSN-L)
- PMC
- PMC11662543
- OpenAlex
- W4405624547
- Cited by
- 5
- References
- 3
- Citations by year
- 2026: 3 · 2025: 2
- FWCI
- 0.82
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Stem Cell Research & Therapy (publishedVersion, cc-by-nc-nd)
- Research Square (acceptedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Natural Science Foundation of China (81873737, 32070586)
- Natural Science Foundation of Shandong Province (ZR2022LZL010)
- Topics
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Laser Applications in Dentistry and Medicine
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Optical Imaging and Spectroscopy Techniques
- Keywords
- near-infrared light, hippocampal neurogenesis, anxiety-like behavior, neural stem cells, astrocyte differentiation, anxiolytic effects, depression prevention, optical stimulation
- MeSH terms
- Animals, Anxiety, Astrocytes, Behavior, Animal, Cell Differentiation, Hippocampus, Infrared Rays, Male, Mice, Inbred C57BL, Cell Proliferation, Mice, Neurogenesis, Neural Stem Cells
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