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Cited by 15T-cell and B-cell ImmunologyGrowth Hormone and Insulin-like Growth FactorsCell Adhesion Molecules Research

Growth hormone in the presence of laminin modulates interaction of human thymic epithelial cells and thymocytes in vitro

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Lins, Marvin Paulo, de Araújo Vieira, Larissa Fernanda, Rosa, Alfredo Aurélio Marinho, Smaniotto, Salete·2016·Biological Research
DOI 10.1186/s40659-016-0097-0PMC PMC5010746
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Abstract

BACKGROUND: Several evidences indicate that hormones and neuropeptides function as immunomodulators. Among these, growth hormone (GH) is known to act on the thymic microenvironment, supporting its role in thymocyte differentiation. The aim of this study was to evaluate the effect of GH on human thymocytes and thymic epithelial cells (TEC) in the presence of laminin. RESULTS: GH increased thymocyte adhesion on BSA-coated and further on laminin-coated surfaces. The number of migrating cells in laminin-coated membrane was higher in GH-treated thymocyte group. In both results, VLA-6 expression on thymocytes was constant. Also, treatment with GH enhanced laminin production by TEC after 24 h in culture. However, VLA-6 integrin expression on TEC remained unchanged. Finally, TEC/thymocyte co-culture model demonstrated that GH elevated absolute number of double-negative (CD4(-)CD8(-)) and single-positive CD4(+) and CD8(+) thymocytes. A decrease in cell number was noted in double-positive (CD4(+)CD8(+)) thymocytes. CONCLUSIONS: The results of this study demonstrate that GH is capable of enhancing the migratory capacity of human thymocytes in the presence of laminin and promotes modulation of thymocyte subsets after co-culture with TEC.

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  • Of the primary articles on thymic cell lines, 43 were published after the report by MacLeod et al. and the most recent one was published only in late 2016.

    “Of the primary articles, 43 were published after the report by MacLeod et al. and the most recent one was published only in late 2016 [41].”

    From The ghosts of HeLa: How cell line misidentification contaminates the scientific literature (2017) · cites this paper as [41]

    The Methods section (page unavailable) confirms that this is a primary study using a human thymic epithelial cell line, so it fits the kind of article cited. Our copy does not establish that it was published in late 2016 or that it was the most recent such article. The count of 43 appears to be the citing authors’ own tally, not a claim made by this source. (LLM, conf=0.78)

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  1. [1]Petrie HT Zúñiga-Pflücker JC Zoned out: functional mapping of stromal signaling microenvironments in the thymus Annu Rev Immunol 2007 25 649 679 10.1146/annurev.immunol.23.021704.115715 17291187 · DOI · Paper page
  2. [2]Griffith AV Fallahi M Nakase H Gosink M Young B Petrie HT Spatial mapping of thymic stromal microenvironments reveals unique features influencing T lymphoid differentiation Immunity 2009 31 999 1009 10.1016/j.immuni.2009.09.024 20064453 PMC2807413 · DOI
  3. [3]Savino W Mendes-Da-Cruz DA Smaniotto S Silva-Monteiro E Villa-Verde DM Molecular mechanisms governing thymocyte migration: combined role of chemokines and extracellular matrix J Leukoc Biol 2004 75 951 961 10.1189/jlb.1003455 15020651 · DOI
  4. [5]Halkias J Melichar HJ Taylor KT Robey EA Tracking migration during human T cell development Cell Mol Life Sci 2014 71 3101 3117 10.1007/s00018-014-1607-2 24682469 PMC11113765 · DOI
  5. [6]Swat W Montgrain V Doggtt TA Douangpanya J Puri K Vermi W Diacovo TG The essential role of PI3K δ and PI3K γ in thymocyte survival Blood 2006 107 2415 2422 10.1182/blood-2005-08-3300 16304053 PMC1895732 · DOI
  6. [7]Ciofani M Zuniga-Pflucker JC The thymus as an inductive site for T lymphopoiesis Annu Rev Cell Dev Biol 2007 23 463 493 10.1146/annurev.cellbio.23.090506.123547 17506693 · DOI
  7. [8]Savino W Mendes-da-Cruz DA Lepletier A Dardenne M Hormonal control of T-cell development in health and disease Nat Rev Endocrinol 2016 12 77 89 26437623
  8. [9]Savino W Dardenne M Neuroendocrine control of the thymus Endocr Rev 2000 21 412 443 10950159
  9. [10]Savino W Neuroendocrine control of T cell development in mammals: role of growth hormone in modulating thymocyte migration Exp Physiol 2007 92 813 817 10.1113/expphysiol.2007.038422 17720747 · DOI
  10. [11]de Mello-Coelho V Villa-Verde DM Dardenne M Savino W Pituitary hormones modulate cell-cell interactions between thymocytes and thymic epithelial cells J Neuroimmunol 1997 76 39 49 10.1016/S0165-5728(97)00031-3 9184631 · DOI
  11. [12]Smaniotto S Mello-Coelho V Villa-Verde DM Pléau JM Postel-Vinay MC Dardenne M Savino W Growth hormone modulates thymocyte development in vivo through a combined action of laminin and CXCL12 Endocrinology 2005 146 3005 3017 10.1210/en.2004-0709 15802502 · DOI
  12. [13]Gameiro J Nagib P Verinaud L The thymus microenvironment in regulating thymocyte differentiation Cell Adh Migr 2010 4 382 390 10.4161/cam.4.3.11789 20418658 PMC2958614 · DOI
  13. [14]Ridley AJ Rho GTPases and cell migration J Cell Sci 2001 114 2713 2722 11683406
  14. [15]Farias-de-Oliveira DA Cotta-de-Almeida V Villa-Verde DM Riederer I Meis JD Savino W Fibronectin modulates thymocyte-thymic epithelial cell interactions following Trypanosoma cruzi infection Mem Inst Oswaldo Cruz 2013 108 825 831 10.1590/0074-0276130071 24271041 PMC3970635 · DOI
  15. [16]Ayres-Martins S Lannes-Vieira J Farias-De-Oliveira DA Brito JM Villa-Verde DMS Savino W Phagocytic cells of the thymic reticulum interact with thymocytes via extracellullar matrix ligands and receptors Cell Immunol 2004 229 21 30 10.1016/j.cellimm.2004.06.002 15331325 · DOI
  16. [17]Golbert DC Santana-van-Vliet E Mundstein AS Calfo V Savino W de Vasconcelos AT Laminin-database v. 2.0: an update on laminins in health and neuromuscular disorders Nucleic Acids Res 2014 42 D426 D429 10.1093/nar/gkt901 24106090 PMC3965114 · DOI
  17. [18]Savino W Dardenne M Pleiotropic modulation of thymic functions by growth hormone: from physiology to therapy Curr Opin Pharmacol 2010 10 434 442 10.1016/j.coph.2010.04.002 20434952 · DOI
  18. [19]Barnard A Layton D Hince M Sakkal S Bernard C Chidgey A Boyd R Impact of the neuroendocrine system on thymus and bone marrow function Neuroimmunomodulation 2008 15 7 18 10.1159/000135619 18667795 · DOI
  19. [21]Smaniotto S Mendes-da-Cruz DA Carvalho-Pinto CE Araujo LM Dardenne M Savino W Combined role of extracellular matrix and chemokines on peripheral lymphocyte migration in growth hormone transgenic mice Brain Behav Immun 2010 24 451 461 10.1016/j.bbi.2009.11.014 19948213 · DOI
  20. [22]Savino W Postel-Vinay MC Smaniotto S Dardenne M The thymus gland: a target organ for growth hormone Scand J Immunol 2002 55 442 452 10.1046/j.1365-3083.2002.01077.x 11975755 · DOI
  21. [23]Vielkind S Gallagher-Gambarelli M Gomez M Hinton HJ Cantrell DA Integrin regulation by RhoA in thymocytes J Immunol 2005 175 350 357 10.4049/jimmunol.175.1.350 15972668 · DOI
  22. [24]Xu X Ge Q Maturation and migration of murine CD4 single-positive thymocytes and thymic emigrants Comput Struct Biotechnol J 2014 9 e201403003 10.5936/csbj.201403003 24757506 PMC3995209 · DOI
  23. [25]Wiedermann CJ Reinisch N Braunsteiner H Stimulation of monocyte chemotaxis by human growth hormone and its deactivation by somatostatin Blood 1993 82 954 960 8101732
  24. [26]Taub DD Tsarfaty G Lloyd AR Durum SK Longo DL Murphy WJ Growth hormone promotes human T cell adhesion and migration to both human and murine matrix proteins in vitro and directly promotes xenogeneic engraftment J Clin Invest 1994 94 293 300 10.1172/JCI117320 7518833 PMC296309 · DOI
  25. [27]Su HW Lanning NJ Morris DL Argetsinger LS Lumeng CN Carter-Su C Phosphorylation of the adaptor protein SH2B1β regulates its ability to enhance growth hormone-dependent macrophage motility J Cell Sci 2013 126 1733 1743 10.1242/jcs.113050 23444381 PMC3678408 · DOI
  26. [28]Smaniotto S Martins-Neto AA Dardenne M Savino W Growth hormone is a modulator of lymphocyte migration Neuroimmunomodulation 2011 18 309 313 10.1159/000329497 21952682 · DOI · Paper page
  27. [29]Yanagawa Y Iwabuchi K Onoe K Enhancement of stromal cell-derived factor-1-induced chemotaxis for CD4/8 double-positive thymocytes by fibronectin and laminin in mice Immunology 2001 104 43 49 10.1046/j.1365-2567.2001.01292.x 11576219 PMC1783275 · DOI
  28. [30]Chaudhry MS Velardi E Dudakov JA van den Brink MR Thymus: the next (re)generation Immunol Rev 2016 271 56 71 10.1111/imr.12418 27088907 PMC4837659 · DOI
  29. [31]Vigano A Saresella M Trabattoni D Giacomet V di Natale B Merlo M Venuto A Villa ML Vanzulli S Ferrante P Clerici M Growth hormone in T-lymphocyte thymic and postthymic development: a study in HIV-infected children J Pediatr 2004 145 542 548 10.1016/j.jpeds.2004.06.027 15480382 · DOI
  30. [32]Napolitano LA Schmidt D Gotway MB Ameli N Filbert EL Ng MM Clor JL Epling L Sinclair E Baum PD Li K Killian ML Bacchetti P McCune JM Growth hormone enhances thymic function in HIV-1-infected adults J Clin Invest 2008 118 1085 1098 18292808 PMC2248326
  31. [33]Bodart G Goffinet L Morrhaye G Farhat K de Saint-Hubert M Debacq-Chainiaux F Swine C Geenen V Martens HJ Somatotrope GHRH/GH/IGF-1 axis at the crossroads between immunosenescence and frailty Ann N Y Acad Sci 2015 1351 61 67 10.1111/nyas.12857 26284958 · DOI
  32. [34]Young CD Angel JB HIV infection of thymocytes inhibits IL-7 activity without altering CD127 expression Retrovirology 2011 8 72 10.1186/1742-4690-8-72 21920046 PMC3182983 · DOI
  33. [35]De Mello-Coelho V Savino W Postel-Vinay MC Dardenne M Role of prolactin and growth hormone on thymus physiology Dev Immunol 1998 6 317 323 10.1155/1998/89782 9814605 PMC2276021 · DOI
  34. [36]Tani-ichi S Shimba A Wagatsuma K Miyachi H Kitano S Imai K Hara T Ikuta K Interleukin-7 receptor controls development and maturation of late stages of thymocyte subpopulations Proc Natl Acad Sci USA 2013 110 612 617 10.1073/pnas.1219242110 23267098 PMC3545802 · DOI
  35. [37]Van De Wiele CJ Marino JH Murray BW Vo SS Whetsell ME Teague TK Thymocytes between the beta-selection and positive selection checkpoints are nonresponsive to IL-7 as assessed by STAT-5 phosphorylation J Immunol 2004 172 4235 4244 10.4049/jimmunol.172.7.4235 15034036 · DOI
  36. [38]Fernández E Vicente A Zapata A Brera B Lozano JJ Martínez C Toribio ML Establishment and characterization of cloned human thymic ephitelial cell lines. Analysis of adhesion molecule expression and cytokine production Blood 1994 83 3245 3254 7514905

Cited by

Sorted by citations

Authors
Lins, M. P., de Araújo Vieira, L. F., Rosa, A. A. M., & Smaniotto, S.
Affiliations
Universidade Federal de Alagoas (BR)
Corresponding author
Smaniotto, Salete
ORCID
Venue
Biological Research
Publisher
Springer Science and Business Media LLC
Published
2016-09-02
Volume
49
Issue
1
Pages
37-37
ISSN
  • 0716-9760 (ISSN-L)
  • 0717-6287
DOI
10.1186/s40659-016-0097-0
PMC
PMC5010746
OpenAlex
W2508682195
Cited by
15
References
2
Citations by year
2025: 2 · 2023: 2 · 2022: 4 · 2020: 2 · 2019: 1 · 2018: 3 · 2017: 1
FWCI
1.01
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Biological Research (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (publishedVersion, other-oa)
  • Americanae (AECID Library) (submittedVersion, other-oa)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Growth Hormone and Insulin-like Growth Factors
  • Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
Keywords
growth hormone, laminin, thymic epithelial cells, thymocytes, thymocyte adhesion, thymocyte migration, thymic microenvironment, thymocyte differentiation, thymocyte subsets, immunomodulation
MeSH terms
Analysis of Variance, Cell Adhesion, Cell Differentiation, Cell Movement, Cells, Cultured, Child, Child, Preschool, Epithelial Cells, Flow Cytometry, Humans, Immunohistochemistry, Infant, Infant, Newborn, Laminin, Reference Values, Growth Hormone, Thymus Gland, Time Factors, CD4-Positive T-Lymphocytes, CD8-Positive T-Lymphocytes, Coculture Techniques, Integrin alpha6beta1, Thymocytes

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