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Cited by 31Growth Hormone and Insulin-like Growth FactorsT-cell and B-cell ImmunologyDevelopmental Biology and Gene Regulation

Expression of the Growth Hormone/Insulin-Like Growth Factor Axis during Balb/c Thymus Ontogeny and Effects of Growth Hormone upon ex vivo T Cell Differentiation

Kermani, Hamid, Goffinet, Lindsay, Mottet, Marie, Bodart, Gwenaelle, Morrhaye, Gabriel, Dardenne, Olivier, Renard, Chantal, Overbergh, Lut +4 more·2012·Neuroimmunomodulation
DOI 10.1159/000328844
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Abstract

Aims: We address the question of the expression and the role of the growth hormone/insulin-like growth factor (GH/IGF) axis in the thymus. Methods: Using RT-qPCR, the expression profile of various components of the somatotrope GH/IGF axis was measured in different thymic cell types and during thymus embryogenesis in Balb/c mice. The effect of GH on T cell differentiation was explored via thymic organotypic culture. Results: Transcription of Gh, Igf1, Igf2 and their related receptors predominantly occurred in thymic epithelial cells (TEC), while a low level of Gh and Igf1r transcription was also evidenced in thymic T cells (thymocytes). Gh, Ghr, Ins2, Igf1, Igf2, and Igfr1 displayed distinct expression profiles depending on the developmental stage. The protein concentrations of IGF-1 and IGF-2 were in accordance with the profile of their gene expression. In fetal thymus organ cultures (FTOC) derived from Balb/c mice, treatment with exogenous GH resulted in a significant increase of double negative CD4–CD8– T cells and CD4+ T cells, together with a decrease in double positive CD4+CD8+ T cells. These changes were inhibited by concomitant treatment with GH and the GH receptor (GHR) antagonist pegvisomant. However, GH treatment also induced a significant decrease in FTOC Gh, Ghr and Igf1 expression. Conclusion: These data show that the thymotropic properties of the somatotrope GH/IGF-1 axis involve an interaction between exogenous GH and GHR expressed by TEC. Since thymic IGF-1 is not increased by GH treatment, the effects of GH upon T cell differentiation could implicate a different local growth factor or cytokine.

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Crossref · 10.1159/000328844

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  • Growth hormone and IGF-1 bind to receptors expressed on thymocytes and thymic epithelial cells.

    “GH and IGF-1 bind to receptors expressed on thymocytes (101) and thymic epithelial cells (102)”

    From Causes, consequences, and reversal of immune system aging (2013) · cites this paper as [102]

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Authors
Kermani, H., Goffinet, L., Mottet, M., Bodart, G., Morrhaye, G., Dardenne, O., Renard, C., Overbergh, L., Baron, F., Beguin, Y., Geenen, V., & Martens, H. J.
Affiliations
  • University of Liège (BE)
  • Institute of Pathology (TH)
  • KU Leuven (BE)
ORCID
Venue
Neuroimmunomodulation
Publisher
S. Karger AG
Published
2012
Volume
19
Issue
3
Pages
137-147
ISSN
  • 1021-7401 (ISSN-L)
  • 1423-0216
DOI
10.1159/000328844
OpenAlex
W2058904843
Cited by
27
References
60
Citations by year
2025: 1 · 2024: 3 · 2023: 4 · 2022: 1 · 2021: 1 · 2020: 3 · 2019: 1 · 2018: 2 · 2017: 1 · 2016: 2 · 2015: 3 · 2014: 3 · 2013: 1 · 2012: 1
FWCI
2.05
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • NeuroImmunoModulation (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Growth Hormone and Insulin-like Growth Factors
  • Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
Keywords
growth hormone, insulin-like growth factor, thymus ontogeny, thymic epithelial cells, T cell differentiation, thymic organ culture, growth hormone receptor, insulin-like growth factor I, insulin-like growth factor II, pegvisomant, double-positive thymocytes, CD4+ T cells, CD4+CD8+ thymocytes
MeSH terms
Animals, Cell Differentiation, Cells, Cultured, Epithelial Cells, Insulin, Insulin-Like Growth Factor I, Insulin-Like Growth Factor II, Mice, Inbred BALB C, Organ Culture Techniques, Receptors, Somatotropin, Growth Hormone, T-Lymphocytes, Thymus Gland, Gene Expression, Receptor, IGF Type 1, Mice, Thymocytes, Real-Time Polymerase Chain Reaction

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