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Decrease of protein phosphatase 2A subunit B by glutamate exposure in the cerebral cortex of neonatal rats

Kang, Ju-Bin, Park, Dong-Ju, Son, Hyun-Kyoung, Koh, Phil-Ok·2020·Laboratory Animal Research
DOI 10.1186/s42826-020-00064-yPMC PMC7501672
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Abstract

Glutamate induces neurotoxicity during brain development, causing nerve damage. Protein phosphatase 2A (PP2A) is a type of serine/threonine phosphatase that regulates various biological functions. Among the PP2A subunit types, subunit B is abundant in brain tissue and plays an essential role in the nervous system. This study investigated changes in PP2A subunit B expression through glutamate exposure in the cerebral cortex of newborn rats. Sprague-Dawley rat pups (7 days after birth) were injected intraperitoneally with vehicle or glutamate (10 mg/kg). After 4 h of drug treatment, the brain tissue was isolated and fixed for morphological study. In addition, the cerebral cortex was collected for RNA and protein works. We observed severe histopathological changes including swollen neuron and atrophied dendrite in the glutamate exposed cerebral cortex. Glutamate exposure leads to a decrease in PP2A subunit B. Reverse-transcription PCR and Western blot analyses confirmed that glutamate induces a decrease of PP2A subunit B in the cerebral cortex of newborn rats. Moreover, immunohistochemical study showed a decrease in PP2A subunit B positive cells. The reduction of PP2A subunit B expression is considered an indicator of neurodegenerative damage. These results suggest that glutamate exposure causes neuronal damage in the cerebral cortex of new born rats through a decrease in PP2A subunit B.

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  • Toxic levels of glutamate cause degenerative cell and neuronal death.

    “These toxic levels of glutamate induce morphological changes in neurons, manifesting in deformed dendrites and expanded rounded neurons (Reynolds’ and Hastings, 1995; Jellinger, 1999; Adamchik et al., 2000; Robert et al., 2002; Youdim et al., 2002; García et al., 2005), showing degenerative cell (Kang et al., 2020; Erkartal et al., 2025) and neuronal death (Weidinger et al., 2023).”

    From Toxic Concentrations of Glutamate Induce Ferroptosis without Affecting Mitochondrial Respiration in Rodent Brain Slice Cultures (2026) · cites this paper as Kang et al., 2020

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Research works printed but not linked: 30

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Cited by

Sorted by citations

Authors
Kang, J.-B., Park, D.-J., Son, H.-K., & Koh, P.-O.
ORCID
Koh, Phil-Ok
Venue
Laboratory Animal Research
Publisher
Springer Science and Business Media LLC
Published
2020-09-18
Volume
36
Issue
1
ISSN
2233-7660
DOI
10.1186/s42826-020-00064-y
PMC
PMC7501672
Cited by
3
References
0
Language
en
Licence
Funders
National Research Foundation of Korea (NRF-2018R1D1A1B07044074)

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