Cited by 307Animal Genetics and ReproductionPhagocytosis and Immune RegulationImmunotoxicology and immune responses

The DOI matches its Crossref record; 47 of the 47 references it cites were found in a registry or an index.

Conditional macrophage ablation in transgenic mice expressing a Fas-based suicide gene

Abstract

Transgenic mice expressing an inducible suicide gene, which allows systemic and reversible elimination of macrophages, were developed. A macrophage-specific c-fms promoter was used to express enhanced green fluorescent protein and a drug-inducible suicide gene that leads to Fas-mediated apoptosis in resting and cycling cells of the macrophage lineage. Transgenic mice were fertile, of normal weight, and showed no abnormal phenotype before drug exposure. The transgene was expressed constitutively in macrophages and dendritic cells (DC) but not significantly in T cells or B cells. Induction of the suicide gene led to depletion of 70–95% of macrophages and DC in nearly all tissues examined. Depletion reduced the ability to clear bacteria from the blood and led to increased bacterial growth in the liver. Depleted mice displayed several abnormalities, including splenomegaly, lymphadenopathy, thymic atrophy, extramedullary hematopoiesis, and development of peritoneal adhesions. This new, transgenic line will be useful in investigating the role of macrophages and DC.

Overview

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Crossref · 10.1189/jlb.0903442

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47 of 47 references verified0 citation errors · 0 not checkable

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Metadata

Authors
Burnett, S. H., Kershen, E. J., Zhang, J., Zeng, L., Straley, S. C., Kaplan, A. M., & Cohen, D. A.
Affiliations
University of Kentucky (US)
Corresponding author
Cohen, Donald A
Venue
Journal of Leukocyte Biology
Published
2004-01-14
Volume
75
Issue
4
Pages
612-623
ISSN
  • 0741-5400 (ISSN-L)
  • 1938-3673
OpenAlex
W2163279811
Cited by
307
References
44
Citations by year
2026: 7 · 2025: 10 · 2024: 21 · 2023: 17 · 2022: 15 · 2021: 17 · 2020: 18 · 2019: 12 · 2018: 17 · 2017: 20 · 2016: 13 · 2015: 22 · 2014: 21 · 2013: 18 · 2012: 11
FWCI
3.20
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Leukocyte Biology (publishedVersion)
  • PubMed (publishedVersion)
Funders
NIH (HL69459, HL57399, 5T32AI049795)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Animal Genetics and Reproduction
  • Life Sciences › Immunology and Microbiology › Immunology › Phagocytosis and Immune Regulation
  • Life Sciences › Immunology and Microbiology › Immunology › Immunotoxicology and immune responses
Keywords
macrophage depletion, dendritic cells, bacterial clearance, splenomegaly, lymphadenopathy, thymic atrophy, extramedullary hematopoiesis, peritoneal adhesions, transgenic mice
MeSH terms
Animals, Bacterial Infections, Cell Count, Cells, Cultured, Dendritic Cells, Disease Models, Animal, Immunity, Cellular, Luminescent Proteins, Macrophages, Mice, Inbred C57BL, Mice, Transgenic, Promoter Regions, Genetic, Receptor, Macrophage Colony-Stimulating Factor, Tacrolimus, Apoptosis, Receptors, Nerve Growth Factor, fas Receptor, Dimerization, Tacrolimus Binding Proteins, Genes, Transgenic, Suicide, Green Fluorescent Proteins, Mice

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