The DOI matches its Crossref record; 47 of the 47 references it cites were found in a registry or an index.
Conditional macrophage ablation in transgenic mice expressing a Fas-based suicide gene
Abstract
Transgenic mice expressing an inducible suicide gene, which allows systemic and reversible elimination of macrophages, were developed. A macrophage-specific c-fms promoter was used to express enhanced green fluorescent protein and a drug-inducible suicide gene that leads to Fas-mediated apoptosis in resting and cycling cells of the macrophage lineage. Transgenic mice were fertile, of normal weight, and showed no abnormal phenotype before drug exposure. The transgene was expressed constitutively in macrophages and dendritic cells (DC) but not significantly in T cells or B cells. Induction of the suicide gene led to depletion of 70–95% of macrophages and DC in nearly all tissues examined. Depletion reduced the ability to clear bacteria from the blood and led to increased bacterial growth in the liver. Depleted mice displayed several abnormalities, including splenomegaly, lymphadenopathy, thymic atrophy, extramedullary hematopoiesis, and development of peritoneal adhesions. This new, transgenic line will be useful in investigating the role of macrophages and DC.
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“BM MΦs play a critical role in bone repair: ablation of macrophages is associated with decreased bone deposition within the fracture callus and higher rates of non-union [13, 14, 24].”
From Osteoimmunology of Fracture Healing. (2024) · cites this paper as [24]
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“Furthermore, an EGFP transgene included in this model enables the in situ fluorescent monitoring of macrophage cells42.”
From Size- and shape-dependent foreign body immune response to materials implanted in rodents and non-human primates (2015) · cites this paper as [42]
In the citing paper
“In this model, a macrophage Fas-induced apoptosis transgene enable inducible/reversible apoptosis of macrophages using the mouse colony stimulating factor 1 receptor promoter (Csf1r) to drive expression of a mutant human FK506 binding protein 1A, 12kDa42.”
From Size- and shape-dependent foreign body immune response to materials implanted in rodents and non-human primates (2015) · cites this paper as [42]
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Peritoneal Adhesions: Etiology, Pathophysiology, and Clinical Significance
Liakakos, T., Thomakos, N., Fine, P. M., Dervenis, C., & Young, R. L. (2001) · Digestive Surgery · doi:10.1159/000050149 · Cited by 687
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Mesothelial cells: Their structure, function and role in serosal repair
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OsteoMacs: Key regulators of bone biology and emerging therapeutic perspectives
Rahalkar, A., & Mani, A. (2026) · Journal of Cellular Biotechnology · doi:10.1177/23523689261448690
Chivers, S. B., McLay, C. L., & Jeske, N. A. (2026) · Molecular Pain · doi:10.1177/17448069261479081
Wang, H., Rajan, V., Wong, A., Epelman, S., & Zúñiga‐Pflücker, J. C. (2026) · eLife · doi:10.7554/elife.109219.2
Calverley, M. (2013) · Montana State University ScholarWorks (Montana State University)
Monnazzi, L. G. S. (2007) · Americanae (AECID Library)
Tumor Associated Macrophages in a MaFIA Mouse Model
Clifford, A. (2006) · ScholarsArchive (Brigham Young University)
Metadata
- Authors
- Burnett, S. H., Kershen, E. J., Zhang, J., Zeng, L., Straley, S. C., Kaplan, A. M., & Cohen, D. A.
- Affiliations
- University of Kentucky (US)
- Corresponding author
- Cohen, Donald A
- Venue
- Journal of Leukocyte Biology
- Publisher
- Oxford University Press (OUP)
- Published
- 2004-01-14
- Volume
- 75
- Issue
- 4
- Pages
- 612-623
- ISSN
- 0741-5400 (ISSN-L)
- 1938-3673
- OpenAlex
- W2163279811
- Cited by
- 307
- References
- 44
- Citations by year
- 2026: 7 · 2025: 10 · 2024: 21 · 2023: 17 · 2022: 15 · 2021: 17 · 2020: 18 · 2019: 12 · 2018: 17 · 2017: 20 · 2016: 13 · 2015: 22 · 2014: 21 · 2013: 18 · 2012: 11
- FWCI
- 3.20
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Leukocyte Biology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- NIH (HL69459, HL57399, 5T32AI049795)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Animal Genetics and Reproduction
- Life Sciences › Immunology and Microbiology › Immunology › Phagocytosis and Immune Regulation
- Life Sciences › Immunology and Microbiology › Immunology › Immunotoxicology and immune responses
- Keywords
- macrophage depletion, dendritic cells, bacterial clearance, splenomegaly, lymphadenopathy, thymic atrophy, extramedullary hematopoiesis, peritoneal adhesions, transgenic mice
- MeSH terms
- Animals, Bacterial Infections, Cell Count, Cells, Cultured, Dendritic Cells, Disease Models, Animal, Immunity, Cellular, Luminescent Proteins, Macrophages, Mice, Inbred C57BL, Mice, Transgenic, Promoter Regions, Genetic, Receptor, Macrophage Colony-Stimulating Factor, Tacrolimus, Apoptosis, Receptors, Nerve Growth Factor, fas Receptor, Dimerization, Tacrolimus Binding Proteins, Genes, Transgenic, Suicide, Green Fluorescent Proteins, Mice
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