Role of Src kinases and Syk in Fcγ receptor-mediated phagocytosis and phagosome-lysosome fusion

Majeed, Meytham; Caveggion, Elena; Lowell, Clifford A; Berton, Giorgio

doi:10.1189/jlb.70.5.801

Cited by 97Phagocytosis and Immune RegulationCellular transport and secretionMonoclonal and Polyclonal Antibodies Research

Role of Src kinases and Syk in Fcγ receptor-mediated phagocytosis and phagosome-lysosome fusion

Majeed, Meytham, Caveggion, Elena, Lowell, Clifford A, Berton, Giorgio·2001·Journal of Leukocyte Biology

DOI 10.1189/jlb.70.5.801

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Abstract

Phagocytosis is increased by Fcγ receptors (FcγRs), and studies with syk−/− macrophages demonstrated that Syk kinase is required for FcγR phagocytosis. Similar studies with macrophages lacking the Src family kinases Hck, Fgr, and Lyn showed that these kinases are not required for phagocytosis but that they enhance the rate of particle engulfment. In this report we show that both wild-type and hck−/−fgr−/− macrophages expressed Fyn, Src, and Yes and that these kinases were activated on ingestion of immunoglobulin G (IgG)-coated particles and redistributed, together with Syk, to actin-rich phagocytic cups and the phagosomal membrane. At doses blocking IgG-dependent phagocytosis, the tyrosine kinase inhibitors PP1 and piceatannol inhibited both Src family kinase and Syk activities, as well as their redistribution to actin-rich phagocytic cups. Hck, Fgr, and Lyn were dispensable for lysosome-phagosome fusion (PLF) induced by IgG-coated particles. However, PP1 or piceatannol hampered unopsonized yeast-induced PLF despite the fact that they did not block yeast internalization.

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Authors: Majeed, M., Caveggion, E., Lowell, C. A., & Berton, G.
Affiliations: University of Verona (IT)
University of California, San Francisco (US)
Corresponding author: Majeed, Meytham
ORCID: Lowell, Clifford A
Venue: Journal of Leukocyte Biology
Publisher: Oxford University Press (OUP)
Published: 2001-11-01
Volume: 70
Issue: 5
Pages: 801-811
ISSN: 0741-5400 (ISSN-L)
1938-3673
DOI: 10.1189/jlb.70.5.801
OpenAlex: W4312243655
Cited by: 97
References: 49
Citations by year: 2026: 8 · 2025: 2 · 2024: 1 · 2023: 2 · 2022: 1 · 2021: 2 · 2020: 3 · 2019: 2 · 2018: 2 · 2017: 3 · 2016: 8 · 2015: 4 · 2014: 3 · 2013: 3 · 2012: 4
FWCI: 1.93
Type: journal article
Language: en
Open access: closed
Licence: academic.oup.com/pages/standard-publication-reuse-rights (vor)
Locations: Journal of Leukocyte Biology (publishedVersion)
Topics: Life Sciences › Immunology and Microbiology › Immunology › Phagocytosis and Immune Regulation
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Monoclonal and Polyclonal Antibodies Research
Keywords: Src family kinases, Syk, Fc gamma receptor, phagocytosis, phagosome-lysosome fusion, macrophages, Hck, Fgr, Lyn kinase, Fyn, Yes, tyrosine kinase inhibitors, protein phosphatase 1, piceatannol, opsonization

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Role of Src kinases and Syk in Fcγ receptor-mediated phagocytosis and phagosome-lysosome fusion

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