The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
A comparison of five lipid extraction solvent systems for lipidomic studies of human LDL
Open accessAbstract
Lipidome profile of fluids and tissues is a growing field as the role of lipids as signaling molecules is increasingly understood, relying on an effective and representative extraction of the lipids present. A number of solvent systems suitable for lipid extraction are commonly in use, though no comprehensive investigation of their effectiveness across multiple lipid classes has been carried out. To address this, human LDL from normolipidemic volunteers was used to evaluate five different solvent extraction protocols [Folch, Bligh and Dyer, acidified Bligh and Dyer, methanol (MeOH)-tert-butyl methyl ether (TBME), and hexane-isopropanol] and the extracted lipids were analyzed by LC-MS in a high-resolution instrument equipped with polarity switching. Overall, more than 350 different lipid species from 19 lipid subclasses were identified. Solvent composition had a small effect on the extraction of predominant lipid classes (triacylglycerides, cholesterol esters, and phosphatidylcholines). In contrast, extraction of less abundant lipids (phosphatidylinositols, lyso-lipids, ceramides, and cholesterol sulfates) was greatly influenced by the solvent system used. Overall, the Folch method was most effective for the extraction of a broad range of lipid classes in LDL, although the hexane-isopropanol method was best for apolar lipids and the MeOH-TBME method was suitable for lactosyl ceramides.
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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION
Bligh, E. G., & Dyer, W. J. (1959) · Canadian Journal of Biochemistry and Physiology · doi:10.1139/o59-099 · Cited by 54,751
Assessment of PLSDA Cross Validation
Westerhuis, J. A., Hoefsloot, H. C. J., Smit, S., Vis, D. J., Smilde, A. K., van Velzen, E. J. J., van Duijnhoven, J. P. M., & van Dorsten, F. A. (2008) · Metabolomics · doi:10.1007/s11306-007-0099-6 · Cited by 1,471
Lipidomics reveals a remarkable diversity of lipids in human plasma
Quehenberger, O., Armando, A. M., Brown, A. H., Milne, S. B., Myers, D. S., Merrill, A. H., Bandyopadhyay, S., Jones, K. N., Kelly, S., Shaner, R. L., et al. (2010) · Journal of Lipid Research · doi:10.1194/jlr.m009449 · Cited by 1,341
Kawai, Y., Kiyokawa, H., Kimura, Y., Kato, Y., Tsuchiya, K., & Terao, J. (2006) · Biochemistry · doi:10.1021/bi0610909 · Cited by 78
Cited by
Advances in Lipid Extraction Methods—A Review
Saini, R. K., Prasad, P., Shang, X., & Keum, Y.-S. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms222413643 · Cited by 479
Liu, X., & Locasale, J. W. (2017) · Trends in Biochemical Sciences · doi:10.1016/j.tibs.2017.01.004 · Cited by 453
Sarafian, M. H., Gaudin, M., Lewis, M. R., Martin, F.-P., Holmes, E., Nicholson, J. K., & Dumas, M.-E. (2014) · Analytical Chemistry · doi:10.1021/ac500317c · Cited by 301
Brügger, B. (2014) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-060713-035324 · Cited by 264
Dysregulation of lipids in Alzheimer’s disease and their role as potential biomarkers
Wong, M. W., Braidy, N., Poljak, A., Pickford, R., Thambisetty, M., & Sachdev, P. S. (2017) · Alzheimer's & Dementia · doi:10.1016/j.jalz.2017.01.008 · Cited by 223
Metadata
- Authors
- Reis, A., Rudnitskaya, A., Blackburn, G. J., Fauzi, N. M., Pitt, A. R., & Spickett, C. M.
- Venue
- Journal of Lipid Research
- Publisher
- Elsevier BV
- Published
- 2013-07
- Volume
- 54
- Issue
- 7
- Pages
- 1812-1824
- ISSN
- 0022-2275 (ISSN-L)
- OpenAlex
- W2118524679
- Cited by
- 241
- References
- 4
- FWCI
- 5.38
- Type
- journal article
- Language
- en
- Open access
- hybrid
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