The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.
Extensive characterization of human tear fluid collected using different techniques unravels the presence of novel lipid amphiphiles
Open accessAbstract
The tear film covers the anterior eye and the precise balance of its various constituting components is critical for maintaining ocular health. The composition of the tear film amphiphilic lipid sublayer, in particular, has largely remained a matter of contention due to the limiting concentrations of these lipid amphiphiles in tears that render their detection and accurate quantitation tedious. Using systematic and sensitive lipidomic approaches, we validated different tear collection techniques and report the most comprehensive human tear lipidome to date; comprising more than 600 lipid species from 17 major lipid classes. Our study confers novel insights to the compositional details of the existent tear film model, in particular the disputable amphiphilic lipid sublayer constituents, by demonstrating the presence of cholesteryl sulfate, O-acyl-ω-hydroxyfatty acids, and various sphingolipids and phospholipids in tears. The discovery and quantitation of the relative abundance of various tear lipid amphiphiles reported herein are expected to have a profound impact on the current understanding of the existent human tear film model.
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Cites
Clinical biochemistry of tears
Van Haeringen, N. J. (1981) · Survey of Ophthalmology · doi:10.1016/0039-6257(81)90145-4 · Cited by 445
de Souza, G. A., de Godoy, L. M., & Mann, M. (2006) · Genome Biology · doi:10.1186/gb-2006-7-8-r72 · Cited by 411
In-depth analysis of the human tear proteome
Zhou, L., Zhao, S. Z., Koh, S. K., Chen, L., Vaz, C., Tanavde, V., Li, X. R., & Beuerman, R. W. (2012) · Journal of Proteomics · doi:10.1016/j.jprot.2012.04.053 · Cited by 379
Therapeutic effects of lysophosphatidylcholine in experimental sepsis
Yan, J.-J., Jung, J.-S., Lee, J.-E., Lee, J., Huh, S.-O., Kim, H.-S., Jung, K. C., Cho, J.-Y., Nam, J.-S., Suh, H.-W., et al. (2004) · Nature Medicine · doi:10.1038/nm989 · Cited by 329
Tear analysis in ocular surface diseases
Zhou, L., & Beuerman, R. W. (2012) · Progress in Retinal and Eye Research · doi:10.1016/j.preteyeres.2012.06.002 · Cited by 318
Identification of Tear Fluid Biomarkers in Dry Eye Syndrome Using iTRAQ Quantitative Proteomics
Zhou, L., Beuerman, R. W., Chan, C. M., Zhao, S. Z., Li, X. R., Yang, H., Tong, L., Liu, S., Stern, M. E., & Tan, D. (2009) · Journal of Proteome Research · doi:10.1021/pr900686s · Cited by 302
Self-citationSapieha, P., Stahl, A., Chen, J., Seaward, M. R., Willett, K. L., Krah, N. M., Dennison, R. J., Connor, K. M., Aderman, C. M., Liclican, E., et al. (2011) · Science Translational Medicine · doi:10.1126/scitranslmed.3001571 · Cited by 252
Investigation of the human tear film proteome using multiple proteomic approaches.
Green‐Church, K. B., Nichols, K. K., Kleinholz, N. M., Zhang, L., & Nichols, J. J. (2008) · PubMed Central · Cited by 183
Butovich, I. A., Wojtowicz, J. C., & Molai, M. (2009) · Journal of Lipid Research · doi:10.1194/jlr.m900252-jlr200 · Cited by 178
On the Lipid Composition of Human Meibum and Tears: Comparative Analysis of Nonpolar Lipids
Butovich, I. A. (2008) · Investigative Opthalmology & Visual Science · doi:10.1167/iovs.08-1889 · Cited by 176
Cited by
TFOS DEWS II Diagnostic Methodology report
Wolffsohn, J. S., Arita, R., Chalmers, R., Djalilian, A., Dogru, M., Dumbleton, K., Gupta, P. K., Karpecki, P., Lazreg, S., Pult, H., et al. (2017) · The Ocular Surface · doi:10.1016/j.jtos.2017.05.001 · Cited by 2,122
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Metadata
- Authors
- Lam, S. M., Tong, L., Duan, X., Petznick, A., Wenk, M. R., & Shui, G.
- Venue
- Journal of Lipid Research
- Publisher
- Elsevier BV
- Published
- 2014-02
- Volume
- 55
- Issue
- 2
- Pages
- 289-298
- ISSN
- 0022-2275 (ISSN-L)
- OpenAlex
- W2018346284
- Cited by
- 310
- References
- 31
- FWCI
- 12.92
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Lam, S. M., Tong, L., Duan, X., Petznick, A., Wenk, M. R., & Shui, G. (2014). Extensive characterization of human tear fluid collected using different techniques unravels the presence of novel lipid amphiphiles. Journal of Lipid Research. https://doi.org/10.1194/jlr.m044826
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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