17α-Hydroxyprogesterone, 4-Androstenedione, and Testosterone Profiled by Routine Stable Isotope Dilution/Gas Chromatography-Mass Spectrometry in Plasma of Children
Abstract
Using stable isotope dilution/gas chromatography-mass spectrometry (ID/GC-MS), a physicochemical method, we have profiled the plasma steroids 17 alpha-hydroxyprogesterone, 4-androstenedione, and testosterone in normal children of various age groups. Comparison of our values with those obtained by direct immunologic assays and those using an extraction or purification step showed that immunoassays in general overestimate steroid concentrations. This was especially true for plasma samples in the neonatal period and was most expressed for the concentrations of 17 alpha-hydroxyprogesterone. Our study demonstrated the applicability of ID/GC-MS to routine clinical steroid analysis. The application of ID/GC-MS is recommended whenever problems from matrix effects or cross-reactivity are likely to arise or suspicious results by immunoassays need to be rechecked.
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Several studies of genotyping of samples from screening programs have suggested that this is a potentially useful adjunct to hormonal measurements, but there has been no large-scale study of its efficacy as a second-tier screen in actual use.
“Several studies of genotyping of samples from screening programs have suggested that this is a potentially useful adjunct to hormonal measurements (277-282), but there has been no large-scale study of its efficacy as a second-tier screen in actual use.”
From Congenital Adrenal Hyperplasia—Current Insights in Pathophysiology, Diagnostics, and Management (2022) · cites this paper as [282]
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- Authors
- Wudy, S. A., Wachter, U. A., Homoki, J., & Teller, W. M.
- Affiliations
- Universität Ulm (DE)
- ORCID
- Wudy, S A
- Venue
- Pediatric Research
- Published
- 1995-07
- Volume
- 38
- Issue
- 1
- Pages
- 76-80
- ISSN
- 0031-3998 (ISSN-L)
- 1530-0447
- OpenAlex
- W2074177744
- Cited by
- 69
- References
- 15
- Citations by year
- 2025: 3 · 2021: 1 · 2020: 1 · 2019: 1 · 2018: 4 · 2017: 3 · 2016: 4 · 2015: 1 · 2014: 3 · 2013: 2 · 2012: 3
- FWCI
- 1.32
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Pediatric Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Deutsche Forschungsgemeinschaft
- Universität Ulm
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adrenal Hormones and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Sexual Differentiation and Disorders
- Health Sciences › Medicine › Physiology › Clinical Laboratory Practices and Quality Control
- Keywords
- 17-hydroxyprogesterone, 4-androstenedione, testosterone, stable isotope dilution, neonatal period, children, matrix effects, cross-reactivity
- MeSH terms
- Adolescent, Androstenedione, Child, Child, Preschool, Female, Humans, Hydroxyprogesterones, Indicator Dilution Techniques, Infant, Infant, Newborn, Isotopes, Male, Gas Chromatography-Mass Spectrometry, Radioimmunoassay, Testosterone, 17-alpha-Hydroxyprogesterone
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