Cited by 69Adrenal Hormones and DisordersSexual Differentiation and DisordersClinical Laboratory Practices and Quality Control

17α-Hydroxyprogesterone, 4-Androstenedione, and Testosterone Profiled by Routine Stable Isotope Dilution/Gas Chromatography-Mass Spectrometry in Plasma of Children

Wudy, S A, Wachter, U A, Homoki, J, Teller, W M·1995·Pediatric Research

Abstract

Using stable isotope dilution/gas chromatography-mass spectrometry (ID/GC-MS), a physicochemical method, we have profiled the plasma steroids 17 alpha-hydroxyprogesterone, 4-androstenedione, and testosterone in normal children of various age groups. Comparison of our values with those obtained by direct immunologic assays and those using an extraction or purification step showed that immunoassays in general overestimate steroid concentrations. This was especially true for plasma samples in the neonatal period and was most expressed for the concentrations of 17 alpha-hydroxyprogesterone. Our study demonstrated the applicability of ID/GC-MS to routine clinical steroid analysis. The application of ID/GC-MS is recommended whenever problems from matrix effects or cross-reactivity are likely to arise or suspicious results by immunoassays need to be rechecked.

Integrity signals

Registry

Crossref · 10.1203/00006450-199507000-00013

Cites

17 of 17 references verified0 citation errors · 0 not checkable

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  • Several studies of genotyping of samples from screening programs have suggested that this is a potentially useful adjunct to hormonal measurements, but there has been no large-scale study of its efficacy as a second-tier screen in actual use.

    “Several studies of genotyping of samples from screening programs have suggested that this is a potentially useful adjunct to hormonal measurements (277-282), but there has been no large-scale study of its efficacy as a second-tier screen in actual use.”

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Authors
Wudy, S. A., Wachter, U. A., Homoki, J., & Teller, W. M.
Affiliations
Universität Ulm (DE)
Venue
Pediatric Research
Published
1995-07
Volume
38
Issue
1
Pages
76-80
ISSN
  • 0031-3998 (ISSN-L)
  • 1530-0447
OpenAlex
W2074177744
Cited by
69
References
15
Citations by year
2025: 3 · 2021: 1 · 2020: 1 · 2019: 1 · 2018: 4 · 2017: 3 · 2016: 4 · 2015: 1 · 2014: 3 · 2013: 2 · 2012: 3
FWCI
1.32
Type
journal article
Language
en
Open access
closed
Locations
  • Pediatric Research (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Deutsche Forschungsgemeinschaft
  • Universität Ulm
Topics
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adrenal Hormones and Disorders
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Sexual Differentiation and Disorders
  • Health Sciences › Medicine › Physiology › Clinical Laboratory Practices and Quality Control
Keywords
17-hydroxyprogesterone, 4-androstenedione, testosterone, stable isotope dilution, neonatal period, children, matrix effects, cross-reactivity
MeSH terms
Adolescent, Androstenedione, Child, Child, Preschool, Female, Humans, Hydroxyprogesterones, Indicator Dilution Techniques, Infant, Infant, Newborn, Isotopes, Male, Gas Chromatography-Mass Spectrometry, Radioimmunoassay, Testosterone, 17-alpha-Hydroxyprogesterone

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