Cited by 706RNA Interference and Gene DeliveryNanoparticle-Based Drug DeliveryMicrobial Inactivation Methods

Nonviral Gene Delivery: Principle, Limitations, and Recent Progress

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Al-Dosari, Mohammed S., Gao, Xiang·2009·The AAPS Journal

Abstract

Gene therapy is becoming a promising therapeutic modality for the treatment of genetic and acquired disorders. Nonviral approaches as alternative gene transfer vehicles to the popular viral vectors have received significant attention because of their favorable properties, including lack of immunogenicity, low toxicity, and potential for tissue specificity. Such approaches have been tested in preclinical studies and human clinical trials over the last decade. Although therapeutic benefit has been demonstrated in animal models, gene delivery efficiency of the nonviral approaches remains to be a key obstacle for clinical applications. This review focuses on existing and emerging concepts of chemical and physical methods for delivery of therapeutic nucleic acid molecules in vivo. The emphasis is placed on discussion about problems associated with current nonviral methods and recent efforts toward refinement of nonviral approaches.

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  1. Wightman L, Kircheis R, Rössler V, Carotta S, Ruzicka R, Kursa M, et al. Different behavior of branched and linear polyethyle- nimine for gene delivery in vitro and in vivo. J Gene Med. 2001;3:362-72.

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Authors
Al-Dosari, M. S., & Gao, X.
Affiliations
  • King Saud University (SA)
  • University of Pittsburgh (US)
Corresponding author
Al-Dosari, Mohammed S.
Venue
The AAPS Journal
Published
2009-10-16
Volume
11
Issue
4
Pages
671-681
ISSN
1550-7416 (ISSN-L)
OpenAlex
W2073026167
Cited by
706
References
102
Citations by year
2026: 25 · 2025: 26 · 2024: 35 · 2023: 34 · 2022: 35 · 2021: 42 · 2020: 56 · 2019: 60 · 2018: 49 · 2017: 53 · 2016: 59 · 2015: 49 · 2014: 49 · 2013: 59 · 2012: 26
FWCI
9.98
Type
journal article
Language
en
Open access
Locations
  • The AAPS Journal (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
  • Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Microbial Inactivation Methods
Keywords
non-viral gene delivery, gene therapy, nucleic acid delivery, transfection, delivery efficiency, clinical translation
MeSH terms
Animals, DNA, Excipients, Genetic Vectors, Humans, Injections, Lipids, Polymers, Ultrasonics, Gene Expression, Drug Delivery Systems, Gene Transfer Techniques, Electroporation, Biolistics, Nanoparticles

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