Nonviral Gene Delivery: Principle, Limitations, and Recent Progress
Open accessAbstract
Gene therapy is becoming a promising therapeutic modality for the treatment of genetic and acquired disorders. Nonviral approaches as alternative gene transfer vehicles to the popular viral vectors have received significant attention because of their favorable properties, including lack of immunogenicity, low toxicity, and potential for tissue specificity. Such approaches have been tested in preclinical studies and human clinical trials over the last decade. Although therapeutic benefit has been demonstrated in animal models, gene delivery efficiency of the nonviral approaches remains to be a key obstacle for clinical applications. This review focuses on existing and emerging concepts of chemical and physical methods for delivery of therapeutic nucleic acid molecules in vivo. The emphasis is placed on discussion about problems associated with current nonviral methods and recent efforts toward refinement of nonviral approaches.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Naked plasmid DNA delivered by electroporation, ultrasound or gene gun risks degradation by serum nucleases and clearance by phagocytosis.
“The simplest gene transfer system is to inject naked plasmid DNA by electroporation, ultrasound or a gene gun [67], although there is a risk of being degraded by nucleases in the serum and cleared by phagocytosis [17].”
From Immune responses to gene editing by viral and non-viral delivery vectors used in retinal gene therapy (2022) · cites this paper as [67]
Non-viral vectors have proven less efficient than viral vectors.
“However, so far, non-viral vectors have proven to be less efficient than viral vectors [67].”
From Immune responses to gene editing by viral and non-viral delivery vectors used in retinal gene therapy (2022) · cites this paper as [67]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Boussif, O., Lezoualc’h, F., Zanta, M. A., Djavaheri‐Mergny, M., Scherman, D., DEMENEIX, B. A., & Behr, J. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.16.7297 · Cited by 6,256 · see citing works
Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure.
Felgner, P. L., Gadek, T. R., Holm, M., Roman, R., Chan, H. W., Wenz, M., Northrop, J. P., RINGOLD, G. M., & Danielsen, M. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.21.7413 · Cited by 4,954 · see citing works
Direct Gene Transfer into Mouse Muscle in Vivo
Wolff, J. A., Malone, R. W., Williams, P., Chong, W., Acsadi, G., Jani, A., & Felgner, P. L. (1990) · Science · doi:10.1126/science.1690918 · Cited by 4,173 · see citing works
Gene transfer into mouse lyoma cells by electroporation in high electric fields.
Neumann, E., Schaefer-Ridder, M., Wang, Y., & HOFSCHNEIDER, P. H. (1982) · The EMBO Journal · doi:10.1002/j.1460-2075.1982.tb01257.x · Cited by 2,510 · see citing works
Hydrodynamics-based transfection in animals by systemic administration of plasmid DNA
Liu, F., Song, Y. K., & Liu, D. (1999) · Gene Therapy · doi:10.1038/sj.gt.3300947 · Cited by 1,728 · see citing works
High-velocity microprojectiles for delivering nucleic acids into living cells
Klein, T. M., Wolf, E. D., Wu, R., & Sanford, J. C. (1987) · Nature · doi:10.1038/327070a0 · Cited by 1,396 · see citing works
Exploring polyethylenimine‐mediated DNA transfection and the proton sponge hypothesis
Akinc, A., Thomas, M., Klibanov, A. M., & Langer, R. (2004) · The Journal of Gene Medicine · doi:10.1002/jgm.696 · Cited by 1,339 · see citing works
An Inverted Hexagonal Phase of Cationic Liposome-DNA Complexes Related to DNA Release and Delivery
Koltover, I., Salditt, T., Rädler, J. O., & Safinya, C. R. (1998) · Science · doi:10.1126/science.281.5373.78 · Cited by 1,336 · see citing works
Fischer, D., Bieber, T., Li, Y., Elsässer, H., & Kissel, T. (1999) · Pharmaceutical Research · doi:10.1023/a:1014861900478 · Cited by 1,219 · see citing works
Mechanism of DNA Release from Cationic Liposome/DNA Complexes Used in Cell Transfection,
Xu, Y., & Szoka, F. C. (1996) · Biochemistry · doi:10.1021/bi9602019 · Cited by 1,136 · see citing works
Chloride Accumulation and Swelling in Endosomes Enhances DNA Transfer by Polyamine-DNA Polyplexes
Sonawane, N. D., Szoka, F. C., & Verkman, A. S. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m308643200 · Cited by 1,083 · see citing works
Current status of polymeric gene delivery systems☆
Park, T. G., JEONG, J., & KIM, S. (2006) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2006.03.007 · Cited by 945 · see citing works
Receptor-mediated in vitro gene transformation by a soluble DNA carrier system.
Wu, G. Y., & Wu, C. H. (1987) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)61209-8 · Cited by 855 · see citing works
Progress in developing cationic vectors for non-viral systemic gene therapy against cancer
Morille, M., Passirani, C., Vonarbourg, A., Clavreul, A., & Benoît, J. (2008) · Biomaterials · doi:10.1016/j.biomaterials.2008.04.036 · Cited by 769 · see citing works
Size-dependent DNA Mobility in Cytoplasm and Nucleus
Lukács, G. L., Haggie, P. M., Seksek, O., Lechardeur, D., Freedman, N. D., & Verkman, A. S. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.275.3.1625 · Cited by 752 · see citing works
Long-term persistence of plasmid DNA and foreign gone expression in mouse muscle
Wolff, J. A., Ludtke, J. J., Acsádi, G., Williams, P. M., & Jáni, Á. (1992) · Human Molecular Genetics · doi:10.1093/hmg/1.6.363 · Cited by 739 · see citing works
Therapeutic applications of ultrasound
ter Haar, G. (2006) · Progress in Biophysics and Molecular Biology · doi:10.1016/j.pbiomolbio.2006.07.005 · Cited by 725 · see citing works
Liposomes for the sustained drug release in vivo
Blume, G., & Cevc, G. (1990) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(90)90440-y · Cited by 701 · see citing works
Different behavior of branched and linear polyethylenimine for gene deliveryin vitro andin vivo
Wightman, L., Kircheis, R., R�ssler, V., Carotta, S., Ruzicka, R., Kursa, M., & Wagner, E. (2001) · The Journal of Gene Medicine · doi:10.1002/jgm.187 · Cited by 696 · see citing works
Li, W. (2004) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2003.10.041 · Cited by 588 · see citing works
Inorganic Nanoparticles as Carriers of Nucleic Acids into Cells
Sokolova, V., & Epple, M. (2007) · Angewandte Chemie International Edition · doi:10.1002/anie.200703039 · Cited by 581 · see citing works
Cationic lipids, lipoplexes and intracellular delivery of genes.
Wasungu, L., & Hoekstra, D. (2006) · Journal of Controlled Release · doi:10.1016/j.jconrel.2006.06.024 · Cited by 575 · see citing works
Yant, S. R., Meuse, L. A., Chiu, W. S., Ivics, Z., Izsvák, Z., & Kay, M. A. (2000) · Nature Genetics · doi:10.1038/75568 · Cited by 509 · see citing works
Sheikov, N. A., McDannold, N. J., Sharma, S., & Hynynen, K. (2008) · Ultrasound in Medicine & Biology · doi:10.1016/j.ultrasmedbio.2007.12.015 · Cited by 494 · see citing works
Losordo, D. W., Vale, P. R., Hendel, R. C., et al. (2002) · Circulation · doi:10.1161/01.cir.0000015982.70785.b7 · Cited by 469 · see citing works
Chen, Z.-Y., He, C.-Y., Ehrhardt, A., & Kay, M. A. (2003) · Molecular Therapy · doi:10.1016/s1525-0016(03)00168-0 · Cited by 463 · see citing works
Targeted magnetic iron oxide nanoparticles for tumor imaging and therapy
Shin, D. (2008) · International Journal of Nanomedicine · doi:10.2147/ijn.s2824 · Cited by 463 · see citing works
Efficient Gene Transfer Using Reversibly Cross-Linked Low Molecular Weight Polyethylenimine
Gosselin, M. A., Guo, W., & Lee, R. J. (2001) · Bioconjugate Chemistry · doi:10.1021/bc0100455 · Cited by 455 · see citing works
Viral Vectors for Gene Transfer
Walther, W., & Stein, U. (2000) · Drugs · doi:10.2165/00003495-200060020-00002 · Cited by 444 · see citing works
Nonviral gene delivery: What we know and what is next
Gao, X., Kim, K., & Liu, D. (2007) · The AAPS Journal · doi:10.1208/aapsj0901009 · Cited by 431 · see citing works
Self-citationAdenovirus enhancement of transferrin-polylysine-mediated gene delivery.
Curiel, D. T., Agarwal, S., Wagner, E., & Cotten, M. L. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.19.8850 · Cited by 423 · see citing works
In vivo electroporation and stable transformation of skin cells of newborn mice by plasmid DNA
Titomirov, A. V., Sukharev, S., & Kistanova, E. K. (1991) · Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression · doi:10.1016/0167-4781(91)90162-f · Cited by 403 · see citing works
Polyethylenimine-based intravenous delivery of transgenes to mouse lung
Goula, D., Benoist, C., Mantero, S., Merlo, G. R., Levi, G., & DEMENEIX, B. A. (1998) · Gene Therapy · doi:10.1038/sj.gt.3300717 · Cited by 374 · see citing works
Characterization of cationic lipid-protamine–DNA (LPD) complexes for intravenous gene delivery
Li, S., Rizzo, M., Bhattacharya, S. S., & Huang, L. (1998) · Gene Therapy · doi:10.1038/sj.gt.3300683 · Cited by 369 · see citing works
Hydrodynamic Gene Delivery: Its Principles and Applications
Suda, T., & Liu, D. (2007) · Molecular Therapy · doi:10.1038/sj.mt.6300314 · Cited by 349 · see citing works
Intracellular trafficking of nonviral vectors
Medina‐Kauwe, L. K., Xie, J., & Hamm‐Alvarez, S. F. (2005) · Gene Therapy · doi:10.1038/sj.gt.3302592 · Cited by 346 · see citing works
Ultrasound-Mediated Transfection of Mammalian Cells
Kim, H. J., Greenleaf, J. F., Kinnick, R. R., et al. (1996) · Human Gene Therapy · doi:10.1089/hum.1996.7.11-1339 · Cited by 339 · see citing works
Nuclear entry of nonviral vectors
Dean, D. A., Strong, D. D., & Zimmer, W. E. (2005) · Gene Therapy · doi:10.1038/sj.gt.3302534 · Cited by 332 · see citing works
Hydroporation as the mechanism of hydrodynamic delivery
Zhang, G., Gao, X., Song, Y. K., Vollmer, R. R., Stolz, D. B., Gasiorowski, J. Z., Dean, D. A., & Liu, D. (2004) · Gene Therapy · doi:10.1038/sj.gt.3302210 · Cited by 331 · see citing works
Self-citationDNA methylation and silencing of gene expression.
Newell-Price, J., Clark, A., & King, P. (2000) · Trends in endocrinology and metabolism · doi:10.1016/s1043-2760(00)00248-4 · Cited by 316 · see citing works
Konstan, M. W., Davis, P. B., Wagener, J. S., Hilliard, K. A., Stern, R. C., Milgram, L. J. H., Kowalczyk, T. H., Hyatt, S. L., Fink, T. L., Gedeon, C. R., et al. (2004) · Human Gene Therapy · doi:10.1089/hum.2004.15.1255 · Cited by 298 · see citing works
DNA electrotransfer: its principles and an updated review of its therapeutic applications
André, F. M., & Mir, L. M. (2004) · Gene Therapy · doi:10.1038/sj.gt.3302367 · Cited by 292 · see citing works
Löser, P., Jennings, G. S., Strauss, M., & Sandig, V. (1998) · Journal of Virology · doi:10.1128/jvi.72.1.180-190.1998 · Cited by 277 · see citing works
Wente, S. R. (2000) · Science · doi:10.1126/science.288.5470.1374 · Cited by 258 · see citing works
Use of ultrasound contrast agents for gene or drug delivery in cardiovascular medicine
Bekeredjian, R., Grayburn, P. A., & Shohet, R. V. (2005) · Journal of the American College of Cardiology · doi:10.1016/j.jacc.2004.08.067 · Cited by 249 · see citing works
Liu, F., Qi, H., Huang, L., & Liu, D. (1997) · Gene Therapy · doi:10.1038/sj.gt.3300424 · Cited by 247 · see citing works
Systemic tumor-targeted gene delivery by anti-transferrin receptor scFv-immunoliposomes.
Xu, L., Huang, C., Huang, W., Tang, W., Rait, A. S., Yin, Y. Z., Cruz, I., Xiang, L., Pirollo, K. F., & Chang, E. H. (2002) · PubMed · Cited by 243 · see citing works
Yew, N. S., Zhao, H., Wu, I., Song, A., Tousignant, J. D., Przybylska, M., & Cheng, S. H. (2000) · Molecular Therapy · doi:10.1006/mthe.2000.0036 · Cited by 221 · see citing works
High-velocity microprojectiles for delivering nucleic acids into living cells. 1987.
Klein, R. M., Wolf, E. D., Wu, R., & Sanford, J. C. (1992) · PubMed · Cited by 221 · see citing works
Acidification of endocytic vesicles by an ATP-dependent proton pump.
Yamashiro, D. J., Fluss, S. R., & Maxfield, F. R. (1983) · The Journal of Cell Biology · doi:10.1083/jcb.97.3.929 · Cited by 218 · see citing works
Efficient Non-Viral Ocular Gene Transfer with Compacted DNA Nanoparticles
Farjo, R., Skaggs, J., Quiambao, A. B., Cooper, M. J., & Naash, M. I. (2006) · PLoS ONE · doi:10.1371/journal.pone.0000038 · Cited by 208 · see citing works
Yew, N. S., Wang, K. X., Przybylska, M., Bagley, R. G., Stedman, M. R., Marshall, J., Scheule, R., & Cheng, S. H. (1999) · Human Gene Therapy · doi:10.1089/10430349950019011 · Cited by 202 · see citing works
Linear DNAs Concatemerize in Vivo and Result in Sustained Transgene Expression in Mouse Liver
Chen, Z.-Y., Yant, S. R., He, C.-Y., Meuse, L. A., Shen, S., & Kay, M. A. (2001) · Molecular Therapy · doi:10.1006/mthe.2001.0278 · Cited by 186 · see citing works
Zhang, G., Song, Y., & Liu, D. (2000) · Gene Therapy · doi:10.1038/sj.gt.3301229 · Cited by 171 · see citing works
Re-Engineering Biopharmaceuticals for Delivery to Brain with Molecular Trojan Horses
Pardridge, W. M. (2008) · Bioconjugate Chemistry · doi:10.1021/bc800148t · Cited by 168 · see citing works
Transfection of airway epithelium by stable PEGylated poly-l-lysine DNA nanoparticles in vivo
Ziady, A. G., Gedeon, C. R., Miller, T. J., Quan, W., Payne, J. M., Hyatt, S. L., Fink, T. L., Muhammad, O., Oette, S. M., Kowalczyk, T., et al. (2003) · Molecular Therapy · doi:10.1016/j.ymthe.2003.07.007 · Cited by 165 · see citing works
Chen, H. H., Ho, Y., Jiang, X., Mao, H., Wang, T., & Leong, K. W. (2008) · Molecular Therapy · doi:10.1038/sj.mt.6300392 · Cited by 155 · see citing works
Pandolfini, T., Rotino, G. L., Camerini, S., Defez, R., & Spena, A. (2002) · BMC Biotechnology · doi:10.1186/1472-6750-2-1 · Cited by 153 · see citing works
Structural advantage of dendritic poly(l-lysine) for gene delivery into cells
Yamagata, M., Kawano, T., Shiba, K., Mori, T., Katayama, Y., & Niidome, T. (2006) · Bioorganic & Medicinal Chemistry · doi:10.1016/j.bmc.2006.09.033 · Cited by 144 · see citing works
Sonoporation using microbubble BR14 promotes pDNA/siRNA transduction to murine heart
Tsunoda, S., Mazda, O., Oda, Y., et al. (2005) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2005.08.052 · Cited by 137 · see citing works
Development of Catheter-Based Procedures for Transducing the Isolated Rabbit Liver with Plasmid DNA
Eastman, S. J., Baskin, K. M., Hodges, B. L., Chu, Q., Gates, A., Dreusicke, R., Anderson, S., & Scheule, R. K. (2002) · Human Gene Therapy · doi:10.1089/10430340260395910 · Cited by 135 · see citing works
The φC31 Integrase System for Gene Therapy
Calos, M. P. (2006) · Current Gene Therapy · doi:10.2174/156652306779010642 · Cited by 133 · see citing works
Physical methods of nucleic acid transfer: general concepts and applications
Julien Villemejane, A., & Mir, L. M. (2009) · British Journal of Pharmacology · doi:10.1111/j.1476-5381.2009.00032.x · Cited by 127 · see citing works
Kol, M. A., van Laak, A. N. C., Rijkers, D. T. S., et al. (2002) · Biochemistry · doi:10.1021/bi0268403 · Cited by 126 · see citing works
Ultrasound-based nonviral gene delivery induces bone formation in vivo
Sheyn, D., Kimelman‐Bleich, N., Pelled, G., et al. (2007) · Gene Therapy · doi:10.1038/sj.gt.3303070 · Cited by 112 · see citing works
Hodges, B. L., Taylor, K. M., Joseph, M. F., Bourgeois, S. A., & Scheule, R. K. (2004) · Molecular Therapy · doi:10.1016/j.ymthe.2004.04.018 · Cited by 112 · see citing works
Cationic polymers for gene delivery: designs for overcoming barriers to systemic administration.
Hwang, S. J., & Davis, M. E. (2001) · PubMed · Cited by 109 · see citing works
Computer-assisted Hydrodynamic Gene Delivery
Suda, T., Suda, K., & Liu, D. (2008) · Molecular Therapy · doi:10.1038/mt.2008.66 · Cited by 107 · see citing works
Sakurai, F., Nishioka, T., Yamashita, F., et al. (2001) · European Journal of Pharmaceutics and Biopharmaceutics · doi:10.1016/s0939-6411(01)00165-5 · Cited by 107 · see citing works
Nanoparticle applications in ocular gene therapy
Cai, X., Conley, S. M., & Naash, M. I. (2007) · Vision Research · doi:10.1016/j.visres.2007.07.012 · Cited by 106 · see citing works
Electroporative transfection with KGF-1 DNA improves wound healing in a diabetic mouse model
Marti, G. P., Ferguson, M. W. J., Wang, J., et al. (2004) · Gene Therapy · doi:10.1038/sj.gt.3302383 · Cited by 105 · see citing works
Inhalable siRNA: Potential as a Therapeutic Agent in the Lungs
Durcan, N., Murphy, C., & Cryan, S. (2008) · Molecular Pharmaceutics · doi:10.1021/mp070048k · Cited by 101 · see citing works
Argyros, O., Wong, S. P., Niceta, M., Waddington, S. N., Howe, S. J., Coutelle, C., Miller, A. D., & Harbottle, R. P. (2008) · Gene Therapy · doi:10.1038/gt.2008.113 · Cited by 96 · see citing works
Fetal Gene Transfer by Intrauterine Injection with Microbubble-Enhanced Ultrasound
Endoh, M., Koibuchi, N., Sato, M., Morishita, R., Kanzaki, T., Murata, Y., & Kaneda, Y. (2002) · Molecular Therapy · doi:10.1006/mthe.2002.0577 · Cited by 94 · see citing works
Lipoplex-Mediated Gene Delivery to the Lung Occurs within 60 Minutes of Intravenous Administration
Barron, L. G., Gagné, L., & Szoka, F. C. (1999) · Human Gene Therapy · doi:10.1089/10430349950017680 · Cited by 90 · see citing works
Complex Formation with Plasmid DNA Increases the Cytotoxicity of Cationic Liposomes
Nguyen, L. T., Atobe, K., Barichello, J. M., Ishida, T., & Kiwada, H. (2007) · Biological and Pharmaceutical Bulletin · doi:10.1248/bpb.30.751 · Cited by 87 · see citing works
Physical Factors Affecting Kinesin-Based Transport of Synthetic Nanoparticle Cargo
Bachand, M., Trent, A. M., Bunker, B. C., et al. (2005) · Journal of Nanoscience and Nanotechnology · doi:10.1166/jnn.2005.112 · Cited by 85 · see citing works
Hydrodynamic gene delivery to the pig liver via an isolated segment of the inferior vena cava
Fabre, J. W., Grehan, A., Whitehorne, M., Sawyer, G. J., Dong, X., Salehi, S., Eckley, L., Zhang, X., Seddon, M. D., Shah, A. M., et al. (2007) · Gene Therapy · doi:10.1038/sj.gt.3303079 · Cited by 76 · see citing works
(2003) · PubMed · Cited by 67 · see citing works
Bastos, R. N., Panté, N., & Burke, B. (1996) · International review of cytology · doi:10.1016/s0074-7696(08)62619-4 · Cited by 65 · see citing works
Song, K.-L., Chang, Y., & Prud’homme, G. J. (2000) · Gene Therapy · doi:10.1038/sj.gt.3301123 · Cited by 65 · see citing works
Vectors for airway gene delivery
Davis, P. B., & Cooper, M. J. (2007) · The AAPS Journal · doi:10.1208/aapsj0901002 · Cited by 63 · see citing works
Freeman, D. J., & Niven, R. W. (1996) · Pharmaceutical Research · doi:10.1023/a:1016078728202 · Cited by 60 · see citing works
Stein, U., Walther, W., Stege, A., Kaszubiak, A., Fichtner, I., & Lage, H. (2007) · Molecular Therapy · doi:10.1038/sj.mt.6300304 · Cited by 59 · see citing works
Photochemically Enhanced Gene Delivery of EGF Receptor-targeted DNA Polyplexes
Kloeckner, J., Prasmickaite, L., Høgset, A., et al. (2004) · Journal of drug targeting · doi:10.1080/10611860410001723090 · Cited by 58 · see citing works
In vivo gene delivery to tumor cells by transferrin‐streptavidin‐DNA conjugate
Sato, Y., Yamauchi, N., Takahashi, M., Sasaki, K., Fukaura, J., Neda, H., Fujii, S., Hirayama, M., Itoh, Y., Koshita, Y., et al. (2000) · The FASEB Journal · doi:10.1096/fj.99-1052com · Cited by 53 · see citing works
Walther, W., Siegel, R., Kobelt, D., et al. (2008) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-08-0412 · Cited by 51 · see citing works
Lysakowski, C., Dumont, L., Tramèr, M. R., et al. (2003) · Anesthesia & Analgesia · doi:10.1097/00000539-200301000-00044 · Cited by 49 · see citing works
Sakai, M., Nishikawa, M., Thanaketpaisarn, O., et al. (2004) · Gene Therapy · doi:10.1038/sj.gt.3302435 · Cited by 48 · see citing works
Hibbitt, O., Harbottle, R. P., Waddington, S. N., et al. (2007) · The Journal of Gene Medicine · doi:10.1002/jgm.1041 · Cited by 47 · see citing works
Low-volume jet injection for intradermal immunization in rabbits
Ren, S. V., Li, M., Smith, J. M., et al. (2002) · BMC Biotechnology · doi:10.1186/1472-6750-2-10 · Cited by 45 · see citing works
Al-Dosari, M. S., Knapp, J. E., & Liu, D. (2005) · Advances in genetics · doi:10.1016/s0065-2660(05)54004-5 · Cited by 44 · see citing works
Self-citationNonionic Amphiphilic Block Copolymers Promote Gene Transfer to the Lung
Desigaux, L., Gourden, C., Bello-Roufaı̈, M., Richard, P., Oudrhiri, N., Lehn, P., Escande, D., Pollard, H., & Pitard, B. (2005) · Human Gene Therapy · doi:10.1089/hum.2005.16.821 · Cited by 43 · see citing works
Goudy, K., Wang, B., & Tisch, R. (2008) · Clinical Immunology · doi:10.1016/j.clim.2008.06.001 · Cited by 39 · see citing works
In Vivo Gene Transfer by Low-Volume Jet Injection
Cartier, R., Ren, S. V., Walther, W., et al. (2000) · Analytical Biochemistry · doi:10.1006/abio.2000.4619 · Cited by 36 · see citing works
Marked Enhancement of Direct Respiratory Tissue Transfection by Aurintricarboxylic Acid
Glasspool‐Malone, J., & Malone, R. W. (1999) · Human Gene Therapy · doi:10.1089/10430349950017707 · Cited by 34 · see citing works
Activation of Human CYP2C9 Promoter and Regulation by CAR and PXR in Mouse Liver
Al-Dosari, M. S., Knapp, J. E., & Liu, D. (2006) · Molecular Pharmaceutics · doi:10.1021/mp0500824 · Cited by 30 · see citing works
Self-citationULTRASOUND MEDIATED INTRAVESICAL TRANSFECTION ENHANCED BY TREATMENT WITH LIDOCAINE OR HEAT
Ogawa, R., Kagiya, G., Feril, L. B., et al. (2004) · The Journal of Urology · doi:10.1097/01.ju.0000139589.52415.3d · Cited by 30 · see citing works
The Effect of Jet Parameters on Jet Injection
Wendell, D. M., Hemond, B. D., Hogan, N., et al. (2006) · doi:10.1109/iembs.2006.260369 · Cited by 25 · see citing works
Uchida, M., Natsume, H., Kobayashi, D., et al. (2002) · Biological and Pharmaceutical Bulletin · doi:10.1248/bpb.25.690 · Cited by 22 · see citing works
Tanaka, A. S., Tanaka, M., & Komuro, K. (1998) · Gene · doi:10.1016/s0378-1119(98)00312-6 · Cited by 11 · see citing works
Harvie (2000) · doi:10.1002/(sici)1520-6017(200005)89:5
Flagged first, then by citations
Research works printed but not linked: 1
- Wightman L, Kircheis R, Rössler V, Carotta S, Ruzicka R, Kursa M, et al. Different behavior of branched and linear polyethyle- nimine for gene delivery in vitro and in vivo. J Gene Med. 2001;3:362-72.
Cited by
Sorted by citations
Endosomal escape pathways for delivery of biologicals
Varkouhi, A. K., Scholte, M., Storm, G., & Haisma, H. J. (2011) · Journal of Controlled Release · doi:10.1016/j.jconrel.2010.11.004 · Cited by 1,561 · see citing works
Novel approaches for vaccine development
Gebre, M. S., Brito, L. A., Tostanoski, L. H., Edwards, D. K., Carfi, A., & Barouch, D. H. (2021) · Cell · doi:10.1016/j.cell.2021.02.030 · Cited by 347 · see citing works
Non-viral strategies for ocular gene delivery
Oliveira, A. V., Rosa da Costa, A. M., & Silva, G. A. (2017) · Materials Science and Engineering: C · doi:10.1016/j.msec.2017.04.068 · Cited by 80 · see citing works
Ren, D., Fisson, S., Dalkara, D., & Ail, D. (2022) · Pharmaceutics · doi:10.3390/pharmaceutics14091973 · Cited by 52 · see citing works
Genetic Modification of the Lung Directed Toward Treatment of Human Disease
Sondhi, D., Stiles, K. M., De, B. P., & Crystal, R. G. (2017) · Human Gene Therapy · doi:10.1089/hum.2016.152 · Cited by 36 · see citing works
Naik, S., Shreya, A. B., Raychaudhuri, R., Pandey, A., Lewis, S. A., Hazarika, M., Bhandary, S. V., Rao, B. S. S., & Mutalik, S. (2021) · Life Sciences · doi:10.1016/j.lfs.2020.118712 · Cited by 33 · see citing works
Gene Therapy for Inherited Retinal Degeneration
Arbabi, A., Liu, A., & Ameri, H. (2019) · Journal of Ocular Pharmacology and Therapeutics · doi:10.1089/jop.2018.0087 · Cited by 30 · see citing works
- Authors
- Al-Dosari, M. S., & Gao, X.
- Affiliations
- King Saud University (SA)
- University of Pittsburgh (US)
- Corresponding author
- Al-Dosari, Mohammed S.
- Venue
- The AAPS Journal
- Published
- 2009-10-16
- Volume
- 11
- Issue
- 4
- Pages
- 671-681
- ISSN
- 1550-7416 (ISSN-L)
- PMC
- PMC2782077
- OpenAlex
- W2073026167
- Cited by
- 706
- References
- 102
- Citations by year
- 2026: 25 · 2025: 26 · 2024: 35 · 2023: 34 · 2022: 35 · 2021: 42 · 2020: 56 · 2019: 60 · 2018: 49 · 2017: 53 · 2016: 59 · 2015: 49 · 2014: 49 · 2013: 59 · 2012: 26
- FWCI
- 9.98
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- The AAPS Journal (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Microbial Inactivation Methods
- Keywords
- non-viral gene delivery, gene therapy, nucleic acid delivery, transfection, delivery efficiency, clinical translation
- MeSH terms
- Animals, DNA, Excipients, Genetic Vectors, Humans, Injections, Lipids, Polymers, Ultrasonics, Gene Expression, Drug Delivery Systems, Gene Transfer Techniques, Electroporation, Biolistics, Nanoparticles
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review