The DOI matches its Crossref record; 20 of the 20 references it cites were found in a registry or an index.
An abundant and specific binding site for the novel vasodilator adrenomedullin in the rat
Abstract
Rat adrenomedullin is a novel 50-amino acid peptide with structural similarities to the calcitonin family of peptides, calcitonin, calcitonin gene-related peptide (CGRP), and islet amyloid polypeptide (IAPP). Using rat [125I]adrenomedullin, specific binding sites were demonstrated in heart, lung, spleen, liver, soleus, diaphragm, gastrocnemius, and spinal cord membranes. The highest binding was present in heart and lung, which was further characterized. These sites exhibited saturation, dissociation, and competition. In rat lung, only rat (IC50 = 5.8 nM) and human (IC50 = 94 nM) adrenomedullin competed with [125I]adrenomedullin. However, in rat heart, rat (IC50 = 0.2 nM) and human (IC50 = 4.2 nM) adrenomedullin, IAPP (IC50 = 240 nM), and CGRP (IC50 = 1050 nM) all competed with [125I] adrenomedullin. Saturation analysis revealed binding capacities and dissociation constants of 2.8 +/- 0.3 pmol/mg protein and 1.3 +/- 0.3 nM, respectively, in lung and 0.47 +/- 0.11 pmol/mg protein and 0.41 +/- 0.14 nM in heart. Comparison with [125I]CGRP- and [125I]IAPP-binding sites in lung showed that rat adrenomedullin could potently inhibit at these sites (IC50 = 5 and 6 nM, respectively). Chemical cross-linking demonstrated a major band of 83,000 mol wt in lung, diaphragm, spleen, and liver and a band of 94,000 mol wt in heart, soleus, and gastrocnemius. Thus, [125I]adrenomedullin-binding sites in rat lung are abundant and can be differentiated from binding sites in rat heart, both pharmacologically and by mol wt.
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Cites
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Cited by
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The endocrine function of the heart
McGrath, M. F., Kuroski de Bold, M. L., & de Bold, A. J. (2005) · Trends in Endocrinology & Metabolism · doi:10.1016/j.tem.2005.10.007 · Cited by 260
Adrenomedullin is a novel adipokine: adrenomedullin in adipocytes and adipose tissues
Li, Y., Jiang, C., Wang, X., Zhang, Y., Shibahara, S., & Takahashi, K. (2007) · Peptides · doi:10.1016/j.peptides.2007.03.005 · Cited by 71
Wong, H. K. (2014) · World Journal of Diabetes · doi:10.4239/wjd.v5.i3.364 · Cited by 51
Metadata
- Authors
- Owji, A. A.
- Venue
- Endocrinology
- Publisher
- The Endocrine Society
- Published
- 1995-05-01
- Volume
- 136
- Issue
- 5
- Pages
- 2127-2134
- ISSN
- 0013-7227 (ISSN-L)
- OpenAlex
- W2039711337
- Cited by
- 310
- References
- 20
- FWCI
- 8.64
- Type
- journal article
- Language
- en
- Open access
- closed
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