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Cited by 106Thyroid Disorders and TreatmentsCardiac electrophysiology and arrhythmiasCardiovascular Function and Risk Factors

The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.

Thyroid hormone regulation of phospholamban phosphorylation in the rat heart

Ojamaa, Kaie, Kenessey, Agnes, Klein, Irwin·2000·Endocrinology
DOI 10.1210/endo.141.6.7514
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Abstract

Thyroid hormone exerts predictable effects on the contractile performance of the heart in part by regulating the transcription of genes encoding specific calcium transporter proteins. In a rat model of hypothyroidism, left ventricular (LV) contractile function as measured by ejection fraction was decreased by 22% (P < 0.05), and this was returned to control values with T3 treatment. In confirmation of prior studies, LV phospholamban (PLB) protein content was significantly decreased by 25% and 40% compared with hypothyroid LV when the animals were treated with T3 at two doses, 2.5 and 7.0 μg/day, respectively. The ratio of sarcoplasmic reticulum calcium adenosine triphosphatase (SERCA2) to PLB protein content was thus increased by 171% and 207%, respectively (P < 0.01). Resolution of the phosphorylated PLB pentamers by SDS-PAGE showed that T3 infusion at 2.5 and 7.0 μg/day decreased (P < 0.001) the amount nonphosphorylated pentamers by 82% and 95%, respectively, in a dose-dependent manner. T3 treatment produced an increase in the proportion of highly phosphorylated PLB pentamers (more than five phosphates) when expressed as a fraction of total pentameric molecules (P < 0.05). Site-specific antibodies showed that the T3-induced increase in phosphorylated PLB pentamers was the result of an increase in both serine 16 and threonine 17 phosphorylation. We conclude that thyroid hormone, in addition to regulating the expression of cardiac PLB, is able to alter the degree of PLB phosphorylation, which correlates with enhancement of LV contractile function. These studies suggest that T3 may augment myocyte calcium cycling via changes in both cAMP- and calcium/calmodulin-dependent protein kinase activities.

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Metadata

Authors
Ojamaa, K., Kenessey, A., & Klein, I.
Affiliations
  • North Shore University Hospital (US)
  • Department of Medicine, Division of Endocrinology, North Shore University Hospital; and Departments of Medicine and Cell Biology, New York University School of Medicine, Manhasset, New York 11030
Corresponding author
Ojamaa, Kaie
ORCID
Ojamaa, Kaie
Venue
Endocrinology
Publisher
The Endocrine Society
Published
2000-06-01
Volume
141
Issue
6
Pages
2139-2144
ISSN
  • 0013-7227 (ISSN-L)
  • 1945-7170
DOI
10.1210/endo.141.6.7514
OpenAlex
W2124633479
Cited by
106
References
25
Citations by year
2024: 3 · 2022: 1 · 2021: 6 · 2020: 4 · 2019: 5 · 2018: 6 · 2017: 2 · 2016: 6 · 2015: 2 · 2014: 7 · 2013: 4 · 2012: 6
FWCI
3.39
Type
journal article
Language
en
Open access
closed
Locations
  • Endocrinology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5k02hl003775-05, 5r01hl058849-03)
  • National Heart, Lung, and Blood Institute (R01HL056804, HL03775, HL-56804, HL58849)
Topics
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Thyroid Disorders and Treatments
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiac electrophysiology and arrhythmias
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiovascular Function and Risk Factors
Keywords
thyroid hormones, triiodothyronine, phospholamban, phospholamban phosphorylation, hypothyroidism, left ventricular contractility, SERCA2, sarcoplasmic reticulum calcium ATPase, calcium handling, protein kinase A, calcium/calmodulin-dependent protein kinase, cardiomyocytes, ejection fraction
MeSH terms
Phospholamban, Calcium-Transporting ATPases, Animals, Calcium-Binding Proteins, Hypothyroidism, Male, Myocardial Contraction, Myocardium, Phosphorylation, Propylthiouracil, RNA, Messenger, Sarcoplasmic Reticulum, Triiodothyronine, Rats, Sprague-Dawley, Rats

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CoChat. (2026). Citation check: Thyroid hormone regulation of phospholamban phosphorylation in the rat heart. https://app.cochat.ai/papers/10.1210/endo.141.6.7514
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Ojamaa, K., Kenessey, A., & Klein, I. (2000). Thyroid hormone regulation of phospholamban phosphorylation in the rat heart. Endocrinology. https://doi.org/10.1210/endo.141.6.7514