CoChat
Sign Up
Cited by 268Estrogen and related hormone effectsHormonal and reproductive studiesIon channel regulation and function

The DOI matches its Crossref record; 46 of the 46 references it cites were found in a registry or an index.

Testosterone Stimulates Intracellular Calcium Release and Mitogen-Activated Protein Kinases Via a G Protein-Coupled Receptor in Skeletal Muscle Cells

Open access
Estrada, Manuel, Espinosa, Alejandra, Müller, Marioly, Jaimovich, Enrique·2003·Endocrinology
DOI 10.1210/en.2002-0164
Open this paper in CoChat — free account

Abstract

Involvement of intracellular Ca(2+) and ERK1/2 phosphorylation in the fast nongenomic effects of androgens in myotubes was investigated. Testosterone or nandrolone produced fast (<1 min) and transient increases in intracellular Ca(2+) with an oscillatory pattern. Calcium signals were slightly reduced in Ca(2+)-free medium, but lack of oscillations was evident. Signals were blocked by U-73122 and xestospongin B, inhibitors of inositol 1,4,5-trisphosphate (IP(3)) pathway. Furthermore, IP(3) increased transiently 2- to 3-fold 45 sec after hormone addition. Cyproterone neither affected the fast Ca(2+) signal nor the increase in IP(3). Calcium increases could also be induced by the impermeant testosterone conjugated to BSA, and the effect of testosterone was abolished in cells incubated with guanosine 5'-O-(2-thiodiphosphate) or pertussis toxin. Stimulation of myotubes with testosterone, nandrolone, or testosterone conjugated to BSA increased immunodetectable phosphorylation of ERK1/2 within 5 min, and this effect was not inhibited by cyproterone. Phosphorylation was blocked by the use of 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid-acetoxymethylester, U-73122, and xestospongin B as well as by dominant negative Ras, MAPK kinase (MEK), or the MEK inhibitor PD-98059. In addition, guanosine 5'-O-(2-thiodiphosphate) or pertussis toxin blocked ERK1/2 phosphorylation. These results are consistent with a fast effect of testosterone, involving a G protein-linked receptor at the plasma membrane, IP(3)-mediated Ca(2+) signal, and the Ras/MEK/ERK pathway in muscle cells.

Overview

Integrity signals

Registry

Crossref · 10.1210/en.2002-0164

Cites

46 of 46 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Flagged first, then by citations

Show all 46 references

Cited by

Sorted by citations

Items 201–268 of 268

Related

See all

Metadata

Authors
Estrada, M., Espinosa, A., Müller, M., & Jaimovich, E.
Affiliations
University of Chile (CL)
Corresponding author
Jaimovich, Enrique
ORCID
Venue
Endocrinology
Publisher
The Endocrine Society
Published
2003-08
Volume
144
Issue
8
Pages
3586-3597
ISSN
  • 0013-7227 (ISSN-L)
  • 1945-7170
DOI
10.1210/en.2002-0164
OpenAlex
W2134223482
Cited by
264
References
46
Citations by year
2026: 6 · 2025: 8 · 2024: 17 · 2023: 14 · 2022: 8 · 2021: 11 · 2020: 13 · 2019: 9 · 2018: 8 · 2017: 8 · 2016: 14 · 2015: 13 · 2014: 8 · 2013: 13 · 2012: 9
FWCI
5.16
Type
journal article
Language
en
Open access
Licence
other-oa
Locations
  • Endocrinology (publishedVersion)
  • PubMed (publishedVersion)
  • Americanae (AECID Library) (submittedVersion, other-oa)
  • LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (submittedVersion, other-oa)
Funders
Olympus
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Estrogen and related hormone effects
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Hormonal and reproductive studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
Keywords
testosterone, nandrolone, intracellular calcium release, calcium oscillations, inositol 1,4,5-trisphosphate, IP3 receptor, ERK1/2 phosphorylation, Ras/MEK/ERK signaling, G protein-coupled receptors, skeletal muscle myotubes, cyproterone, pertussis toxin, MEK inhibitors
MeSH terms
Androgens, Animals, Animals, Newborn, Calcium, Chelating Agents, Nandrolone, Phosphorylation, Testosterone, Transfection, Calcium Channels, Inositol 1,4,5-Trisphosphate, Gene Expression, Protein Serine-Threonine Kinases, Cyclic AMP Response Element-Binding Protein, Cyproterone Acetate, Receptors, Cytoplasmic and Nuclear, Muscle, Skeletal, Muscle Fibers, Skeletal, ras Proteins, GTP-Binding Proteins, Mitogen-Activated Protein Kinase 1, Mitogen-Activated Protein Kinases, Mitogen-Activated Protein Kinase 3, Rats, Inositol 1,4,5-Trisphosphate Receptors, Androgen Receptor Antagonists

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review