The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
Kisspeptin Cells in the Ewe Brain Respond to Leptin and Communicate with Neuropeptide Y and Proopiomelanocortin Cells
Open accessAbstract
Kisspeptin stimulates reproduction, and kisspeptin cells in the arcuate nucleus (ARC) express Ob-Rb in the mouse. Herein we report studies in ewes to determine whether kisspeptin cells express Ob-Rb and respond to leptin and whether reciprocal connections exist between kisspeptin cells and proopiomelanocortin (POMC) or neuropeptide Y (NPY) cells to modulate reproduction and metabolic function. Kiss1 mRNA was measured by in situ hybridization in ovariectomized ewes that were normal body weight, lean, or lean with leptin treatment by intracerebroventricular (icv) infusion (4 μg/h, 3 d). Kiss1 expression in the ARC and the preoptic area was lower in hypogonadotropic lean animals than animals of normal weight, and icv infusion of leptin partially restored Kiss1 expression in lean animals. Single-cell laser capture microdissection coupled with real-time PCR showed that Kiss1 cells in the preoptic area and ARC express Ob-Rb. Double-label fluorescent immunohistochemistry showed that reciprocal connections exist between kisspeptin cells and NPY and POMC cells. Accordingly, we treated ovariectomized ewes with kisspeptin (5 μg/h, icv) or vehicle for 20 h and examined POMC and NPY gene expression by in situ hybridization. Kisspeptin treatment reduced POMC and increased NPY gene expression. Thus, kisspeptin neurons respond to leptin and expression of Kiss1 mRNA is affected by leptin status. Kisspeptin cells communicate with NPY and POMC cells, altering expression of the relevant genes in the target cells; reciprocal connections also exist. This network between the three cell types could coordinate brain control of reproduction and metabolic homeostatic systems.
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Cites
Hypogonadotropic hypogonadism due to loss of function of the KiSS1-derived peptide receptor GPR54
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“phenotype of metabolic disruption (69).”
Anatomy and regulation of the central melanocortin system
Cone, R. D. (2005) · Nature Neuroscience · doi:10.1038/nn1455 · Cited by 1,583
Glucose sensing by POMC neurons regulates glucose homeostasis and is impaired in obesity
Parton, L. E., Ye, C. P., Coppari, R., Enriori, P. J., Choi, B., Zhang, C.-Y., Xu, C., Vianna, C. R., Balthasar, N., Lee, C. E., et al. (2007) · Nature · doi:10.1038/nature06098 · Cited by 700
Kisspeptin neurons in the arcuate nucleus of the ewe express both dynorphin A and neurokinin B
Goodman, R. L., Lehman, M. N., Smith, J. T., Coolen, L. M., de Oliveira, C. V. R., Jafarzadehshirazi, M. R., Pereira, A., Iqbal, J., Caraty, A., Ciofi, P., et al. (2007) · Endocrinology · doi:10.1210/en.2007-0961 · Cited by 692
Self-citationRegulation of hypothalamic proopiomelanocortin mRNA by leptin in ob/ob mice
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“Similarly, leptin positively regulates POMC mRNA expression in rodent species (20, 26).”
BackgroundSmith, J. T., Clay, C. M., Caraty, A., & Clarke, I. J. (2007) · Endocrinology · doi:10.1210/en.2006-1435 · Cited by 404
Caraty, A., Smith, J. T., Lomet, D., Ben Saïd, S., Morrissey, A., Cognie, J., Doughton, B., Baril, G., Briant, C., & Clarke, I. J. (2007) · Endocrinology · doi:10.1210/en.2007-0554 · Cited by 313
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“The cDNA and plasmid inserts used were a 375-base ovine Kiss1 sequence (31), a 400-base ovine POMC sequence (32), and a 511-base rat NPY sequence donated by Dr.”
MethodSelf-citation
Cited by
Characterization of Kiss1 neurons using transgenic mouse models
Cravo, R. M., Margatho, L. O., Osborne-Lawrence, S., Donato, J., Atkin, S., Bookout, A. L., Rovinsky, S., Frazão, R., Lee, C. E., Gautron, L., et al. (2011) · Neuroscience · doi:10.1016/j.neuroscience.2010.11.022 · Cited by 353
Metabolic regulation of kisspeptin — the link between energy balance and reproduction
Navarro, V. M. (2020) · Nature Reviews Endocrinology · doi:10.1038/s41574-020-0363-7 · Cited by 240
Female reproduction and type 1 diabetes: From mechanisms to clinical findings
Codner, E., Merino, P. M., & Tena-Sempere, M. (2012) · Human Reproduction Update · doi:10.1093/humupd/dms024 · Cited by 182
Neuroanatomy of the kisspeptin signaling system in mammals: comparative and developmental aspects
Lehman, M. N., Hileman, S. M., & Goodman, R. L. (2013) · Advances in Experimental Medicine and Biology · doi:10.1007/978-1-4614-6199-9_3 · Cited by 180
Manfredi-Lozano, M., Roa, J., & Tena-Sempere, M. (2018) · Frontiers in Neuroendocrinology · doi:10.1016/j.yfrne.2017.07.008 · Cited by 162
Metadata
- Authors
- Backholer, K., Smith, J. T., Rao, A., Pereira, A., Iqbal, J., Ogawa, S., Li, Q., & Clarke, I. J.
- Venue
- Endocrinology
- Publisher
- The Endocrine Society
- Published
- 2010-03-05
- Volume
- 151
- Issue
- 5
- Pages
- 2233-2243
- ISSN
- 0013-7227 (ISSN-L)
- 1945-7170
- OpenAlex
- W2084810464
- Cited by
- 289
- References
- 9
- FWCI
- 23.76
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Backholer, K., Smith, J. T., Rao, A., Pereira, A., Iqbal, J., Ogawa, S., Li, Q., & Clarke, I. J. (2010). Kisspeptin Cells in the Ewe Brain Respond to Leptin and Communicate with Neuropeptide Y and Proopiomelanocortin Cells. Endocrinology. https://doi.org/10.1210/en.2009-1190
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