The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.
1,25(OH)2Vitamin D3 Stimulates Myogenic Differentiation by Inhibiting Cell Proliferation and Modulating the Expression of Promyogenic Growth Factors and Myostatin in C2C12 Skeletal Muscle Cells
Open accessAbstract
Skeletal muscle wasting is an important public health problem associated with aging, chronic disease, cancer, kidney dialysis, and HIV/AIDS. 1,25-Dihydroxyvitamin D (1,25-D3), the active form of vitamin D, is widely recognized for its regulation of calcium and phosphate homeostasis in relation to bone development and maintenance and for its calcemic effects on target organs, such as intestine, kidney, and parathyroid glands. Emerging evidence has shown that vitamin D administration improves muscle performance and reduces falls in vitamin D-deficient older adults. However, little is known of the underlying mechanism or the role 1,25-D3 plays in promoting myogenic differentiation at the cellular and/or molecular level. In this study, we examined the effect of 1,25-D3 on myoblast cell proliferation, progression, and differentiation into myotubes. C2C12 myoblasts were treated with 1,25-D3 or placebo for 1, 3, 4, 7, and 10 d. Vitamin D receptor expression was analyzed by quantitative RT-PCR, Western blottings and immunofluorescence. Expression of muscle lineage, pro- and antimyogenic, and proliferation markers was assessed by immunocytochemistry, PCR arrays, quantitative RT-PCR, and Western blottings. Addition of 1,25-D3 to C2C12 myoblasts 1) increased expression and nuclear translocation of the vitamin D receptor, 2) decreased cell proliferation, 3) decreased IGF-I expression, and 4) promoted myogenic differentiation by increasing IGF-II and follistatin expression and decreasing the expression of myostatin, the only known negative regulator of muscle mass, without changing growth differentiation factor 11 expression. This study identifies key vitamin D-related molecular pathways for muscle regulation and supports the rationale for vitamin D intervention studies in select muscle disorder conditions.
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Citations
Cites
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Double muscling in cattle due to mutations in the myostatin gene
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Vitamin D Status Predicts Physical Performance and Its Decline in Older Persons
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Vitamin D Receptor Expression in Human Muscle Tissue Decreases with Age
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Follistatin complexes Myostatin and antagonises Myostatin-mediated inhibition of myogenesis
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Regulation of Muscle Mass by Follistatin and Activins
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Cited by
Molecular Mechanisms of Vitamin D Action
Haussler, M. R., Whitfield, G. K., Kaneko, I., Haussler, C. A., Hsieh, D., Hsieh, J.-C., & Jurutka, P. W. (2013) · Calcified Tissue International · doi:10.1007/s00223-012-9619-0 · Cited by 759
The roles of vitamin D in skeletal muscle: form, function, and metabolism
Girgis, C. M., Clifton-Bligh, R. J., Hamrick, M. W., Holick, M. F., & Gunton, J. E. (2013) · Endocrine Reviews · doi:10.1210/er.2012-1012 · Cited by 567
Plausible ergogenic effects of vitamin D on athletic performance and recovery
Dahlquist, D. T., Dieter, B. P., & Koehle, M. S. (2015) · Journal of the International Society of Sports Nutrition · doi:10.1186/s12970-015-0093-8 · Cited by 151
Englund, D. A., Kirn, D. R., Koochek, A., Zhu, H., Travison, T. G., Reid, K. F., von Berens, Å., Melin, M., Cederholm, T., Gustafsson, T., et al. (2018) · The Journals of Gerontology: Series A · doi:10.1093/gerona/glx141 · Cited by 151
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Metadata
- Authors
- Garcia, L. A., King, K. K., Ferrini, M. G., Norris, K. C., & Artaza, J. N.
- Venue
- Endocrinology
- Publisher
- The Endocrine Society
- Published
- 2011-06-14
- Volume
- 152
- Issue
- 8
- Pages
- 2976-2986
- ISSN
- 0013-7227 (ISSN-L)
- 1945-7170
- OpenAlex
- W2070216811
- Cited by
- 263
- References
- 8
- FWCI
- 6.47
- Type
- journal article
- Language
- en
- Open access
- bronze
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