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Cited by 15Bone health and osteoporosis researchVitamin D Research StudiesGrowth Hormone and Insulin-like Growth Factors

Low calcium-phosphate intakes modulate the low-protein diet-related effect on peak bone mass acquisition: a hormonal and bone strength determinants study in female growing rats

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Fournier, C., Rizzoli, R., Ammann, P.·2014·Endocrinology
DOI 10.1210/en.2014-1308
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Abstract

Peak bone mass acquisition is influenced by environmental factors including dietary intake. A low-protein diet delays body and skeletal growth in association with a reduction in serum IGF-1 whereas serum FGF21 is increased by selective amino acid deprivation. Calcium (Ca) and phosphorous (P) are also key nutrients for skeletal health, and inadequate intakes reduce bone mass accrual in association with calciotropic hormone modulation. Besides, the effect of calcium supplementation on bone mass in prepubertal children appears to be influenced by protein intake. To further explore the interaction of dietary protein and Ca-P intake on bone growth, 1-month-old female rats were fed with an isocaloric 10%, 7.5%, or 5% casein diet containing normal or low Ca-P for an 8-week period (6 groups). Changes in tibia geometry, mineral content, microarchitecture, strength, and intrinsic bone quality were analyzed. At the hormonal level, serum IGF-1, fibroblast growth factor 21 (FGF21), PTH, 1,25-dihydroxyvitamin D3 (calcitriol), and FGF23 were investigated as well as the Ghr hepatic gene expression. In normal dietary Ca-P conditions, bone mineral content, trabecular and cortical bone volume, and bone strength were lower in the 5% casein group in association with a decrease in serum IGF-1 and an increase in FGF21 levels. Unexpectedly, the low-Ca-P diet attenuated the 5% casein diet-related reduction of serum IGF-1 and Ghr hepatic gene expression, as well as the low-protein diet-induced decrease in bone mass and strength. However, this was associated with lower cortical bone material level properties. The low-Ca-P diet increased serum calcitriol but decreased FGF23 levels. Calcitriol levels positively correlated with Ghr hepatic mRNA levels. These results suggest that hormonal modulation in response to a low-Ca-P diet may modify the low-protein diet-induced effect on Ghr hepatic mRNA levels and consequently the impact of low protein intakes on IGF-1 circulating levels and skeletal growth.

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  • Liver FGF21 expression and circulating FGF21 protein levels are increased by the restriction of protein intake in mice, rats, and humans.

    “liver FGF21 expression and circulating FGF21 protein levels are increased by the restriction of protein intake in mice, rats, and humans”

    From FGF21 and the physiological regulation of macronutrient preference (2020) · cites this paper as [149]

  • Reductions in dietary protein intake-or the intake of specific amino acids-increase liver FGF21 mRNA expression and circulating FGF21 protein levels.

    “reductions in dietary protein intake—or the intake of specific amino acids—increase liver FGF21 mRNA expression and circulating FGF21 protein levels”

    From FGF21 and the physiological regulation of macronutrient preference (2020) · cites this paper as [149]

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Authors
Fournier, C., Rizzoli, R., & Ammann, P.
Affiliations
University Hospital of Geneva (CH)
Corresponding author
Fournier, C.
ORCID
Venue
Endocrinology
Publisher
The Endocrine Society
Published
2014-11-01
Volume
155
Issue
11
Pages
4305-4315
ISSN
  • 0013-7227 (ISSN-L)
  • 1945-7170
DOI
10.1210/en.2014-1308
OpenAlex
W2158444447
Cited by
15
References
55
Citations by year
2026: 1 · 2024: 1 · 2023: 1 · 2022: 2 · 2020: 4 · 2019: 3 · 2018: 1 · 2016: 1 · 2015: 1
FWCI
0.69
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Endocrinology (publishedVersion)
  • PubMed (publishedVersion)
  • Archive ouverte UNIGE (University of Geneva) (submittedVersion, cc-by)
Topics
  • Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
  • Health Sciences › Medicine › Pathology and Forensic Medicine › Vitamin D Research Studies
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Growth Hormone and Insulin-like Growth Factors
Keywords
peak bone mass, low-protein diet, dietary protein, insulin-like growth factor I, FGF21, growth hormone receptor, bone strength, bone mineral content, bone microarchitecture, 1,25-dihydroxyvitamin D3, fibroblast growth factor 23, parathyroid hormone, tibia, casein diet
MeSH terms
Animals, Body Composition, Bone and Bones, Bone Development, Calcium Phosphates, Calcium, Dietary, Female, Hormones, Weight Gain, Bone Density, Rats, Sprague-Dawley, Diet, Protein-Restricted, Compressive Strength, Growth and Development, Rats

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