Simultaneous Measurement of Luteinizing Hormone-Releasing Hormone and Luteinizing Hormone Release in Unanesthetized, Ovariectomized Sheep*
Abstract
The push-pull perfusion technique was used in combination with a sequential bleeding schedule to estimate simultaneously the release patterns of LHRH and LH in unanesthetized ovariectomized sheep and to determine the temporal relationship between the release of these two hormones. Ovariectomized (greater than 30 days) ewes received unilateral push-pull cannula (PPC) implants (od, 0.85 mm) into the median eminence. After at least 6 days of recovery, each ewe was fitted with an indwelling jugular catheter. For push-pull perfusion, a stylette was removed from the outer PPC, and an inner cannula assembly (od, 0.40 mm) was inserted. Artificial cerebrospinal fluid was pushed through the inner cannula and pulled up between the cannulae at 20 microliters/min. Continuous 10-min perfusate fractions were collected, acidified, and stored at -20 C for LHRH RIA. Blood samples were obtained every 10 min via the jugular catheter, each being drawn 5 min after the start of a perfusate collection interval. Plasma LH levels were determined by RIA. The duration of the sampling was 3-7 h. LHRH output was distinctly pulsatile, occurring at a frequency of approximately one pulse every 30-40 min (n = 5 sheep). LHRH pulse amplitude and frequency remained relatively constant throughout each perfusion. Plasma LH values also were pulsatile, and all LH peaks occurred either during the same interval or during the interval after a LHRH pulse. LH pulses always were accompanied or directly preceded by LHRH pu…
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- Authors
- LEVINE, J. E., PAU, K.-Y. F., RAMIREZ, V. D., & JACKSON, G. L.
- Affiliations
- University of Illinois Urbana-Champaign (US)
- ORCID
- LEVINE, JON E.
- Venue
- Endocrinology
- Publisher
- The Endocrine Society
- Published
- 1982-11
- Volume
- 111
- Issue
- 5
- Pages
- 1449-1455
- ISSN
- 0013-7227 (ISSN-L)
- 1945-7170
- OpenAlex
- W1978308275
- Cited by
- 384
- References
- 1
- Citations by year
- 2025: 2 · 2024: 2 · 2023: 1 · 2022: 5 · 2021: 4 · 2020: 2 · 2019: 3 · 2018: 7 · 2017: 7 · 2016: 3 · 2015: 4 · 2014: 4 · 2013: 14 · 2012: 7
- FWCI
- 18.66
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
- Social Sciences › Psychology › Social Psychology › Neuroendocrine regulation and behavior
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Keywords
- gonadotropin-releasing hormone, luteinizing hormone, pulsatile hormone secretion, median eminence, hypothalamic-pituitary-gonadal axis, ovariectomy, sheep, push-pull perfusion, LH pulse amplitude, LH pulse frequency
- MeSH terms
- Animals, Castration, Female, Kinetics, Luteinizing Hormone, Gonadotropin-Releasing Hormone, Median Eminence, Perfusion, Sheep
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