Estrogen Receptor-β Immunoreactivity in Luteinizing Hormone-Releasing Hormone Neurons of the Rat Brain
Abstract
Feedback regulation of luteinizing hormone-releasing hormone (LHRH) neurons by estradiol plays important roles in the neuroendocrine control of reproduction. Recently, we found that the majority of LHRH neurons in the rat contain estrogen receptor-beta (ER-beta) mRNA, whereas, they seemed to lack ER-alpha mRNA expression. In addition, we observed nuclear uptake of (125)I-estrogen by a subset of these cells. These data suggest that ER-beta is the chief receptor isoform mediating direct estrogen effects upon LHRH neurons. To verify the translation of ER-beta protein within LHRH cells, the present studies applied dual-label immunocytochemistry (ICC) to free-floating sections obtained from the preoptic area of rats. The improved ICC method using the silver-gold intensification of nickel-diaminobenzidine chromogen, enabled the observation of nuclear ER-beta-immunoreactivity in the majority of LHRH cells. The incidence of ER-beta expression was similarly high in LHRH neurons of ovariectomized female (87.8 +/- 2.3%, mean +/- SEM), estradiol-primed female (74.9 +/- 3.2%) and intact male (85.0 +/- 4.7%) rats. The presence of ER-beta mRNA, ER-beta immunoreactivity and (125)I-estrogen binding sites in LHRH neurons of the rat provide strong support for the notion that these cells are directly regulated by estradiol, through ER-beta. The gene targets and molecular mechanisms of this regulation remain unknown.
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Some immunohistochemistry studies in rat brain have detected ERβ in GnRH neurons.
“Some studies using immunohistochemistry in rat brain have indeed shown the presence of ERβ in GnRH neuron (Butler, Sjoberg, & Coen, 1999; Hrabovszky et al., 2007; Hrabovszky et al., 2001; Kallo, Butler, Barkovics-Kallo, Goubillon, & Coen, 2001; Navarro et al., 2003; Roy, Angelini, & Belsham, 1999).”
From Hypothalamic–pituitary–adrenal and hypothalamic–pituitary–gonadal axes: sex differences in regulation of stress responsivity (2017) · cites this paper as Hrabovszky et al., 2001
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“Some studies using immunohistochemistry in rat brain have indeed shown the presence of ERb in GnRH neuron (Butler, Sjoberg, & Coen, 1999; Hrabovszky et al., 2007; Hrabovszky et al., 2001; Kallo, Butler, Barkovics-Kallo, Goubillon, & Coen, 2001; Navarro et al., 2003; Roy, Angelini, & Belsham, 1999).”
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- Authors
- Hrabovszky, E., Steinhauser, A., Barabás, K., Shughrue, P. J., Petersen, S. L., Merchenthaler, I., & Liposits, Z.
- Affiliations
- HUN-REN Institute of Experimental Medicine (HU)
- Department of Neurobiology (E.H., A.S., K.B., Z.S.L.) Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, 1083 Hungary
- The Women’s Health Research Institute (P.J.S., I.M.), Wyeth-Ayerst Research, Radnor, Pennsylvania 19087
- University of Massachusetts Amherst (US)
- Venue
- Endocrinology
- Publisher
- The Endocrine Society
- Published
- 2001-07-01
- Volume
- 142
- Issue
- 7
- Pages
- 3261-3264
- ISSN
- 0013-7227 (ISSN-L)
- 1945-7170
- OpenAlex
- W2024788152
- Cited by
- 258
- References
- 0
- Citations by year
- 2026: 3 · 2025: 1 · 2024: 2 · 2023: 3 · 2022: 4 · 2021: 4 · 2020: 5 · 2019: 6 · 2018: 10 · 2017: 10 · 2016: 9 · 2015: 7 · 2014: 9 · 2013: 10 · 2012: 13
- FWCI
- 18.23
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (HD-27305)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Social Sciences › Psychology › Social Psychology › Neuroendocrine regulation and behavior
- Keywords
- estrogen receptor beta, luteinizing hormone-releasing hormone neurons, preoptic area, neuroendocrine regulation of reproduction, estrogen binding sites, ovariectomy
- MeSH terms
- Animals, Brain, Estradiol, Female, Immunohistochemistry, Gonadotropin-Releasing Hormone, Male, Neurons, Ovariectomy, Receptors, Estrogen, RNA, Messenger, Rats, Wistar, In Situ Hybridization, Estrogen Receptor beta, Rats
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